Restriction map for
pFN21AE2056
(Circular) (Six-base) MAPSORT of: pFN21AE2056.seq Check: 2692 from: 1 to: 6781
pFN21AE2056 6781 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3507 3877 3507
Size: 370 6411
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3735 4591 278
Size: 53 83 186 936 2199 856 2468
Fragments arranged by size:
2468 2199 936 856 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4124 1937
Size: 2187 4594
Acc65I G'GTAC_C
Cuts at: 4102 4102
Size: 6781
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4053 4844 6228 6496 1632
Size: 2421 791 1384 268 1917
Fragments arranged by size:
2421 1917 1384 791 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6584
AflIII A'CryG_T
Cuts at: 1203 5680 6657 1203
Size: 4477 977 1327
Fragments arranged by size:
4477 1327 977
AgeI A'CCGG_T
Cuts at: 5612 5612
Size: 6781
AhdI GACnn_n'nnGTC
Cuts at: 2311 2479 5510 2311
Size: 168 3031 3582
Fragments arranged by size:
3582 3031 168
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2633 3293 1184
Size: 1449 660 4672
Fragments arranged by size:
4672 1449 660
AlwNI CAG_nnn'CTG
Cuts at: 2418 3877 3950 4524 6096 2418
Size: 1459 73 574 1572 3103
Fragments arranged by size:
3103 1572 1459 574 73
ApaI G_GGCC'C
Cuts at: 2221 2718 2221
Size: 497 6284
ApaLI G'TGCA_C
Cuts at: 4838 5994 4838
Size: 1156 5625
ApoI r'AATT_y
Cuts at: 1493 3032 3165 3239 4090 4316 4468 6577 1493
Size: 1539 133 74 851 226 152 2109 1697
Fragments arranged by size:
2109 1697 1539 851 226 152 133 74
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 2535 2961 4106 1870
Size: 73 592 426 1145 4545
Fragments arranged by size:
4545 1145 592 426 73
BamHI G'GATC_C
Cuts at: 4111 4478 4111
Size: 367 6414
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2609 3495 3889 3959
Size: 325 217 627 333 489 886 394 70
Cuts at: 3959 4102 5557 618
Size: 143 1455 1842
Fragments arranged by size:
1842 1455 886 627 489 394 333 325
217 143 70
BanII G_rGCy'C
Cuts at: 1391 2221 2378 2549 2661 2718 4100 1391
Size: 830 157 171 112 57 1382 4072
Fragments arranged by size:
4072 1382 830 171 157 112 57
BbeI G_GCGC'C
Cuts at: 2124 3963 2124
Size: 1839 4942
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5810
BbvCI CC'TCA_GC
Cuts at: 3816 3816
Size: 6781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4972 5006 1903
Size: 34 3035 34 3678
Fragments arranged by size:
3678 3035 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4673 5883 4673
Size: 1210 5571
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6781
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 5392 136
Size: 107 122 71 631 605 517 3203 1525
Fragments arranged by size:
3203 1525 631 605 517 122 107 71
BglII A'GATC_T
Cuts at: 6776 6776
Size: 6781
BlpI GC'TnA_GC
Cuts at: 4198 4198
Size: 6781
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2640 2718 3894 3994 4842 5998 1790
Size: 431 419 78 1176 100 848 1156 2573
Fragments arranged by size:
2573 1176 1156 848 431 419 100 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6781
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2746 4512 5470
Size: 416 53 83 399 24 1329 1766 958
Cuts at: 5470 442
Size: 1753
Fragments arranged by size:
1766 1753 1329 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 6781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 3287 3473 3777 5441 1239
Size: 1156 892 186 304 1664 2579
Fragments arranged by size:
2579 1664 1156 892 304 186
Bpu10I CC'TnA_GC
Cuts at: 3019 3816 3019
Size: 797 5984
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3608 3846 4906 5771 6069 3608
Size: 238 1060 865 298 4320
Fragments arranged by size:
4320 1060 865 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 3245 5444 882
Size: 617 1021 725 2199 2219
Fragments arranged by size:
2219 2199 1021 725 617
BsaAI yAC'GTr
Cuts at: 493 1204 2692 4572 493
Size: 711 1488 1880 2702
Fragments arranged by size:
2702 1880 1488 711
BsaBI GATnn'nnATC
Cuts at: 2859 6775 2859
Size: 3916 2865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 3346 3732 3918
Size: 53 83 186 936 588 1225 386 186
Cuts at: 3918 3960 4588 4970 5605 275
Size: 42 628 382 635 1451
Fragments arranged by size:
1451 1225 936 635 628 588 386 382
186 186 83 53 42
BsaWI w'CCGG_w
Cuts at: 2595 5214 5612 5886 6033 6444 2595
Size: 2619 398 274 147 411 2932
Fragments arranged by size:
2932 2619 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3488 3518 1178
Size: 30 165 30 2085 30 4441
Fragments arranged by size:
4441 2085 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2887 2954 3090 3476 3731 1186
Size: 1701 67 136 386 255 4236
Fragments arranged by size:
4236 1701 386 255 136 67
BseYI C'CCAG_C
Cuts at: 2167 2447 2530 2723 4015 5984 6480 2167
Size: 280 83 193 1292 1969 496 2468
Fragments arranged by size:
2468 1969 1292 496 280 193 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3174 3456 3485 1163
Size: 573 1438 282 29 4459
Fragments arranged by size:
4459 1438 573 282 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2214 2488 4231 4992 6020 1540
Size: 275 186 213 274 1743 761 1028 2301
Fragments arranged by size:
2301 1743 1028 761 275 274 213 186
BsiHKAI G_wGCw'C
Cuts at: 2378 2433 3417 4842 4927 5998 2378
Size: 55 984 1425 85 1071 3161
Fragments arranged by size:
3161 1425 1071 984 85 55
BsmI GAATG_Cn'
Cuts at: 2525 4292 4385 2525
Size: 1767 93 4921
Fragments arranged by size:
4921 1767 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2378 2433 2549 2640 2661 2718
Size: 399 431 157 55 116 91 21 57
Cuts at: 2718 3417 3894 3994 4100 4842 4927 5998 1391
Size: 699 477 100 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 699 477 431 399 157
116 106 100 91 85 57 55 21
BspEI T'CCGG_A
Cuts at: 6444 6444
Size: 6781
BspHI T'CATG_A
Cuts at: 4670 6400 4670
Size: 1730 5051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 3507 3519 3877 4136 844
Size: 1212 1451 12 358 259 3489
Fragments arranged by size:
3489 1451 1212 358 259 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 2542 2835 3018 3791 4668 1394
Size: 429 517 202 293 183 773 877 3507
Fragments arranged by size:
3507 877 773 517 429 293 202 183
BsrDI GCAATG_nn'
Cuts at: 65 1248 2280 2497 5276 5450 65
Size: 1183 1032 217 2779 174 1396
Fragments arranged by size:
2779 1396 1183 1032 217 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2727 3811 3848 5425 5612 1700
Size: 110 917 1084 37 1577 187 2869
Fragments arranged by size:
2869 1577 1084 917 187 110 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6781
BssSI C'ACGA_G
Cuts at: 2173 4841 5853 2173
Size: 2668 1012 3101
Fragments arranged by size:
3101 2668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3989 794
Size: 3195 3586
BstBI TT'CG_AA
Cuts at: 5585 5585
Size: 6781
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6781
BstXI CCAn_nnnn'nTGG
Cuts at: 2175 2233 2175
Size: 58 6723
BstYI r'GATC_y
Cuts at: 1972 3216 3388 4111 4478 4863 4880 6332 6418
Size: 1244 172 723 367 385 17 1452 86
Cuts at: 6418 6430 6776 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1244 723 385 367 346 172
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3714 3714
Size: 6781
BtgI C'CryG_G
Cuts at: 513 1065 2004 2757 3291 3507 3726 3831 4023
Size: 552 939 753 534 216 219 105 192
Cuts at: 4023 513
Size: 3271
Fragments arranged by size:
3271 939 753 552 534 219 216 192
105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6042
Fragments arranged by size:
6042 683 56
BtsI GCAGTG_nn'
Cuts at: 3442 3765 4297 5091 5111 3442
Size: 323 532 794 20 5112
Fragments arranged by size:
5112 794 532 323 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6781
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6746
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DraIII CAC_nnn'GTG
Cuts at: 3508 3508
Size: 6781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5788 809
Size: 507 4472 1802
Fragments arranged by size:
4472 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2211 2729
Size: 54 1425 50 275 18 252 129 518
Cuts at: 2729 2830 3406 3723 4228 5117 6746 8
Size: 101 576 317 505 889 1629 43
Fragments arranged by size:
1629 1425 889 576 518 505 317 275
252 129 101 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2211 4228 1537
Size: 275 399 2017 4090
Fragments arranged by size:
4090 2017 399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2750 3104 3127 3207 3876 4711 1353
Size: 1397 354 23 80 669 835 3423
Fragments arranged by size:
3423 1397 835 669 354 80 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5368 5742 5888 387
Size: 1064 138 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2376 2376
Size: 6781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2395 3287 3473 3777 4053 4844 5441
Size: 393 763 892 186 304 276 791 597
Cuts at: 5441 6228 6496 1239
Size: 787 268 1524
Fragments arranged by size:
1524 892 791 787 763 597 393 304
276 268 186
EcoNI CCTnn'n_nnAGG
Cuts at: 3396 3396
Size: 6781
EcoO109I rG'GnC_Cy
Cuts at: 2217 2218 2415 2704 2714 2715 3634 3827 2217
Size: 1 197 289 10 1 919 193 5171
Fragments arranged by size:
5171 919 289 197 193 10 1 1
EcoRI G'AATT_C
Cuts at: 3032 3165 4090 4468 3032
Size: 133 925 378 5345
Fragments arranged by size:
5345 925 378 133
FspI TGC'GCA
Cuts at: 2621 5287 2621
Size: 2666 4115
HaeII r_GCGC'y
Cuts at: 1678 2124 3788 3963 5928 1678
Size: 446 1664 175 1965 2531
Fragments arranged by size:
2531 1965 1664 446 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2465 2497 2648
Size: 32 135 32 725 32 136 32 151
Cuts at: 2648 2680 2839 2871 3002 3034 4040 4072 5422
Size: 32 159 32 131 32 1006 32 1350
Cuts at: 5422 5454 5496 5528 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 1006 725 159 151 136 135
131 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2968 4125 4371 669
Size: 988 281 1030 1157 246 3079
Fragments arranged by size:
3079 1157 1030 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6781
HpaI GTT'AAC
Cuts at: 4371 4371
Size: 6781
KasI G'GCGC_C
Cuts at: 2120 3959 2120
Size: 1839 4942
KpnI G_GTAC'C
Cuts at: 4106 4106
Size: 6781
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2295 2508 2953 5895 6079 1305
Size: 990 213 445 2942 184 2007
Fragments arranged by size:
2942 2007 990 445 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 3408 10
Size: 54 1425 343 252 1324 3383
Fragments arranged by size:
3383 1425 1324 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2615 4739 5098 5257 518
Size: 648 1065 384 2124 359 159 2042
Fragments arranged by size:
2124 2042 1065 648 384 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2645 3005 3493 3728 3804 3842 3874
Size: 482 557 360 488 235 76 38 32
Cuts at: 3874 4019 4025 4197 4874 6022 6267 1606
Size: 145 6 172 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 557 488 482 360 245
235 172 145 76 38 32 6
NaeI GCC'GGC
Cuts at: 2729 3813 2729
Size: 1084 5697
NarI GG'CG_CC
Cuts at: 2121 3960 2121
Size: 1839 4942
NcoI C'CATG_G
Cuts at: 513 1065 2004 2757 513
Size: 552 939 753 4537
Fragments arranged by size:
4537 939 753 552
NdeI CA'TA_TG
Cuts at: 387 2068 387
Size: 1681 5100
NgoMIV G'CCGG_C
Cuts at: 2727 3811 2727
Size: 1084 5697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6781
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 2788 3463 4139 5684 1132
Size: 97 1559 675 676 1545 2229
Fragments arranged by size:
2229 1559 1545 676 675 97
PasI CC'CwG_GG
Cuts at: 1348 2059 1348
Size: 711 6070
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 2673 2758 1108
Size: 1194 371 85 5131
Fragments arranged by size:
5131 1194 371 85
PfoI T'CCnGG_A
Cuts at: 2589 3303 2589
Size: 714 6067
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 6363 6395 4850
Size: 32 1481 32 5236
Fragments arranged by size:
5236 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3634 3634
Size: 6781
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6781
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PspOMI G'GGCC_C
Cuts at: 2217 2714 2217
Size: 497 6284
PstI C_TGCA'G
Cuts at: 830 3946 4133 830
Size: 3116 187 3478
Fragments arranged by size:
3478 3116 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6781
PvuII CAG'CTG
Cuts at: 2088 3005 3874 4019 2088
Size: 917 869 145 4850
Fragments arranged by size:
4850 917 869 145
SacI G_AGCT'C
Cuts at: 2378 2378
Size: 6781
SacII CC_GC'GG
Cuts at: 3729 4026 3729
Size: 297 6484
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SapI GCTCTTCn'nnn_
Cuts at: 3876 3876
Size: 6781
SbfI CC_TGCA'GG
Cuts at: 4133 4133
Size: 6781
ScaI AGT'ACT
Cuts at: 5029 6741 5029
Size: 1712 5069
SfcI C'TryA_G
Cuts at: 826 1045 2155 2452 3942 4129 5264 5945 6136
Size: 219 1110 297 1490 187 1135 681 191
Cuts at: 6136 826
Size: 1471
Fragments arranged by size:
1490 1471 1135 1110 681 297 219 191
187
SfiI GGCCn_nnn'nGGCC
Cuts at: 2189 2189
Size: 6781
SfoI GGC'GCC
Cuts at: 2122 3961 2122
Size: 1839 4942
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6781
SmaI CCC'GGG
Cuts at: 1872 2537 2963 4108 1872
Size: 665 426 1145 4545
Fragments arranged by size:
4545 1145 665 426
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3623 3861 4885 5786 6048 820
Size: 197 926 1680 238 1024 901 262 1553
Fragments arranged by size:
1680 1553 1024 926 901 262 238 197
SnaBI TAC'GTA
Cuts at: 493 4572 493
Size: 4079 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 2788 4139 1132
Size: 1656 1351 3774
Fragments arranged by size:
3774 1656 1351
SspI AAT'ATT
Cuts at: 5 52 4705 5
Size: 47 4653 2081
Fragments arranged by size:
4653 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6781
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2316 2757 3924 4220 513
Size: 552 939 183 129 441 1167 296 3074
Fragments arranged by size:
3074 1167 939 552 441 296 183 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 2684 4808 4825 4978 5163 5608
Size: 1255 492 612 2124 17 153 185 445
Cuts at: 5608 325
Size: 1498
Fragments arranged by size:
2124 1498 1255 612 492 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 5027 6739
Size: 274 80 33 51 1443 534 2516 1712
Cuts at: 6739 96
Size: 138
Fragments arranged by size:
2516 1712 1443 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 3488 3520 6741 6773 2064
Size: 32 1392 32 3221 32 2072
Fragments arranged by size:
3221 2072 1392 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6781
XbaI T'CTAG_A
Cuts at: 4117 4117
Size: 6781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2066 3565 2066
Size: 1499 5282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6781
XmaI C'CCGG_G
Cuts at: 1870 2535 2961 4106 1870
Size: 665 426 1145 4545
Fragments arranged by size:
4545 1145 665 426
XmnI GAAnn'nnTTC
Cuts at: 2924 4910 2924
Size: 1986 4795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3733 4589 276
Size: 53 83 186 936 2199 856 2468
Fragments arranged by size:
2468 2199 936 856 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI