Restriction map for
pFN21AE2056
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MaeI MfeI
MluI MroI MseI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 1726 GACGTC GACGT^C
AfaI 507 GTAC GT^AC
AocI 1708 CCTGAGG CC^T[ACGT]AGG
Asp700I 915 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 614 TGCGCA TGC^GCA
BbvCI 1810 GCTGAGG CCTCAGC(-5/-2)
BglI 178 GCCGCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 685 TACGTG [CT]AC^GT[GA]
BsaBI 850 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 590 ACCGGA [AT]^CCGG[AT]
BsmI 514 GAATGC GAATGC(1/-1)
BssSI 168 CTCGTG CACGAG(-5/-1)
BstAPI 1978 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1708 CCTGAGG CC^T[ACGT]AGG
DraIII 1498 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 206 CGGCCG C^GGCCG
EclXI 206 CGGCCG C^GGCCG
Eco52I 206 CGGCCG C^GGCCG
Eco81I 1708 CCTGAGG CC^T[ACGT]AGG
EcoNI 1387 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 614 TGCGCA TGC^GCA
HincII 961 GTTGAC GT[CT]^[GA]AC
HindII 961 GTTGAC GT[CT]^[GA]AC
MamI 850 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 752 CCATGG C^CATGG
NdeI 62 CATATG CA^TATG
PpuMI 1628 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1937 CTGCAG CTGCA^G
RsaI 507 GTAC GT^AC
SacI 369 GAGCTC GAGCT^C
SapI 1876 GAAGAGC GCTCTTC(1/4)
SfiI 177 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 779 GCATGC GCATG^C
XmnI 915 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AhdI 301 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
469 GACCTGGGGTC
ApaI 212 GGGCCC GGGCC^C
709 GGGCCC
AspEI 301 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
469 GACCTGGGGTC
AvaI 530 CCCGGG C^[CT]CG[GA]G
956 CCCGGG
BalI 77 TGGCCA TGG^CCA
1401 TGGCCA
BbeI 115 GGCGCC GGCGC^C
1954 GGCGCC
BsaI 521 GAGACC GGTCTC(1/5)
1246 GAGACC
BsiEI 206 CGGCCG CG[GA][CT]^CG
480 CGACCG
BsoBI 530 CCCGGG C^[CT]CG[GA]G
956 CCCGGG
BsrDI 276 CATTGC GCAATG(2/0)
485 GCAATG
BstXI 163 CCAGCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
221 CCATGCACATGG
BstYI 1211 AGATCC [GA]^GATC[CT]
1383 AGATCC
Eam1105I 301 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
469 GACCTGGGGTC
EcoRI 1027 GAATTC G^AATTC
1160 GAATTC
KasI 115 GGCGCC G^GCGCC
1954 GGCGCC
KspI 1721 CCGCGG CCGC^GG
2018 CCGCGG
MaeII 686 ACGT A^CGT
1727 ACGT
MaeIII 133 GTCAC ^GT[ACGT]AC
1004 GTGAC
MflI 1211 AGATCC [GA]^GATC[CT]
1383 AGATCC
MluNI 77 TGGCCA TGG^CCA
1401 TGGCCA
MscI 77 TGGCCA TGG^CCA
1401 TGGCCA
MslI 222 CATGCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
606 CACCTTCATG
NaeI 722 GCCGGC GCC^GGC
1806 GCCGGC
NarI 115 GGCGCC GG^CGCC
1954 GGCGCC
NgoMIV 722 GCCGGC G^CCGGC
1806 GCCGGC
NspI 779 GCATGC [GA]CATG^[CT]
1454 ACATGC
SacII 1721 CCGCGG CCGC^GG
2018 CCGCGG
SfoI 115 GGCGCC GGC^GCC
1954 GGCGCC
SmaI 530 CCCGGG CCC^GGG
956 CCCGGG
SmlI 1618 CTCAAG C^T[CT][GA]AG
1856 CTCAAG
Tsp45I 133 GTCAC ^GT[GC]AC
1004 GTGAC
XcmI 54 CCAGGGTGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1553 CCAGTCTCCATGTGG
XhoII 1211 AGATCC [GA]^GATC[CT]
1383 AGATCC
XmaCI 530 CCCGGG C^CCGGG
956 CCCGGG
XmaI 530 CCCGGG C^CCGGG
956 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp509I TspRI TthHB8I Van91I