(Linear) (Six-base) MAPSORT of: KIEE2056.seq Check: 7994 from: 1 to: 2076 KIEE2056.orf With 182 enzymes: SgfI * July 3, 2012 17:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1502 1872 2076 Size: 1502 370 204 Fragments arranged by size: 1502 370 204 AatII G_ACGT'C Cuts at: 0 1730 2076 Size: 1730 346 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2048 2076 Size: 2048 28 AhdI GACnn_n'nnGTC Cuts at: 0 306 474 2076 Size: 306 168 1602 Fragments arranged by size: 1602 306 168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 628 1288 2076 Size: 628 660 788 Fragments arranged by size: 788 660 628 AlwNI CAG_nnn'CTG Cuts at: 0 413 1872 1945 2076 Size: 413 1459 73 131 Fragments arranged by size: 1459 413 131 73 ApaI G_GGCC'C Cuts at: 0 216 713 2076 Size: 216 497 1363 Fragments arranged by size: 1363 497 216 ApoI r'AATT_y Cuts at: 0 1027 1160 1234 2076 Size: 1027 133 74 842 Fragments arranged by size: 1027 842 133 74 AvaI C'yCGr_G Cuts at: 0 530 956 2076 Size: 530 426 1120 Fragments arranged by size: 1120 530 426 BanI G'GyrC_C Cuts at: 0 115 604 1490 1884 1954 2076 Size: 115 489 886 394 70 122 Fragments arranged by size: 886 489 394 122 115 70 BanII G_rGCy'C Cuts at: 0 216 373 544 656 713 2076 Size: 216 157 171 112 57 1363 Fragments arranged by size: 1363 216 171 157 112 57 BbeI G_GCGC'C Cuts at: 0 119 1958 2076 Size: 119 1839 118 Fragments arranged by size: 1839 119 118 BbvCI CC'TCA_GC Cuts at: 0 1811 2076 Size: 1811 265 BglI GCCn_nnn'nGGC Cuts at: 0 184 2076 Size: 184 1892 Bme1580I G_kGCm'C Cuts at: 0 216 635 713 1889 1989 2076 Size: 216 419 78 1176 100 87 Fragments arranged by size: 1176 419 216 100 87 78 BmrI ACTGGGnnnn_n' Cuts at: 0 741 2076 Size: 741 1335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 558 2076 Size: 558 1518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 390 1282 1468 1772 2076 Size: 390 892 186 304 304 Fragments arranged by size: 892 390 304 304 186 Bpu10I CC'TnA_GC Cuts at: 0 1014 1811 2076 Size: 1014 797 265 Fragments arranged by size: 1014 797 265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1603 1841 2076 Size: 1603 238 235 Fragments arranged by size: 1603 238 235 BsaI GGTCTCn'nnnn_ Cuts at: 0 515 1240 2076 Size: 515 725 836 Fragments arranged by size: 836 725 515 BsaAI yAC'GTr Cuts at: 0 687 2076 Size: 687 1389 BsaBI GATnn'nnATC Cuts at: 0 854 2076 Size: 854 1222 BsaHI Gr'CG_yC Cuts at: 0 116 1341 1727 1913 1955 2076 Size: 116 1225 386 186 42 121 Fragments arranged by size: 1225 386 186 121 116 42 BsaWI w'CCGG_w Cuts at: 0 590 2076 Size: 590 1486 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1483 1513 2076 Size: 1483 30 563 Fragments arranged by size: 1483 563 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 882 949 1085 1471 1726 2076 Size: 882 67 136 386 255 350 Fragments arranged by size: 882 386 350 255 136 67 BseYI C'CCAG_C Cuts at: 0 162 442 525 718 2010 2076 Size: 162 280 83 193 1292 66 Fragments arranged by size: 1292 280 193 162 83 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1169 1451 1480 2076 Size: 1169 282 29 596 Fragments arranged by size: 1169 596 282 29 BsiEI CG_ry'CG Cuts at: 0 209 483 2076 Size: 209 274 1593 Fragments arranged by size: 1593 274 209 BsiHKAI G_wGCw'C Cuts at: 0 373 428 1412 2076 Size: 373 55 984 664 Fragments arranged by size: 984 664 373 55 BsmI GAATG_Cn' Cuts at: 0 520 2076 Size: 520 1556 Bsp1286I G_dGCh'C Cuts at: 0 216 373 428 544 635 656 713 1412 Size: 216 157 55 116 91 21 57 699 Cuts at: 1412 1889 1989 2076 Size: 477 100 87 Fragments arranged by size: 699 477 216 157 116 100 91 87 57 55 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 51 1502 1514 1872 2076 Size: 51 1451 12 358 204 Fragments arranged by size: 1451 358 204 51 12 BsrBI CCG'CTC Cuts at: 0 335 537 830 1013 1786 2076 Size: 335 202 293 183 773 290 Fragments arranged by size: 773 335 293 290 202 183 BsrDI GCAATG_nn' Cuts at: 0 275 492 2076 Size: 275 217 1584 Fragments arranged by size: 1584 275 217 BsrFI r'CCGG_y Cuts at: 0 722 1806 1843 2076 Size: 722 1084 37 233 Fragments arranged by size: 1084 722 233 37 BssSI C'ACGA_G Cuts at: 0 168 2076 Size: 168 1908 BstAPI GCAn_nnn'nTGC Cuts at: 0 1984 2076 Size: 1984 92 BstXI CCAn_nnnn'nTGG Cuts at: 0 170 228 2076 Size: 170 58 1848 Fragments arranged by size: 1848 170 58 BstYI r'GATC_y Cuts at: 0 1211 1383 2076 Size: 1211 172 693 Fragments arranged by size: 1211 693 172 Bsu36I CC'TnA_GG Cuts at: 0 1709 2076 Size: 1709 367 BtgI C'CryG_G Cuts at: 0 752 1286 1502 1721 1826 2018 2076 Size: 752 534 216 219 105 192 58 Fragments arranged by size: 752 534 219 216 192 105 58 BtsI GCAGTG_nn' Cuts at: 0 1437 1760 2076 Size: 1437 323 316 Fragments arranged by size: 1437 323 316 DraIII CAC_nnn'GTG Cuts at: 0 1503 2076 Size: 1503 573 EaeI y'GGCC_r Cuts at: 0 77 206 724 825 1401 1718 2076 Size: 77 129 518 101 576 317 358 Fragments arranged by size: 576 518 358 317 129 101 77 EagI C'GGCC_G Cuts at: 0 206 2076 Size: 206 1870 EarI CTCTTCn'nnn_ Cuts at: 0 745 1099 1122 1202 1871 2076 Size: 745 354 23 80 669 205 Fragments arranged by size: 745 669 354 205 80 23 EcoICRI GAG'CTC Cuts at: 0 371 2076 Size: 371 1705 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 390 1282 1468 1772 2048 2076 Size: 390 892 186 304 276 28 Fragments arranged by size: 892 390 304 276 186 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 1391 2076 Size: 1391 685 EcoO109I rG'GnC_Cy Cuts at: 0 212 213 410 699 709 710 1629 1822 Size: 212 1 197 289 10 1 919 193 Cuts at: 1822 2076 Size: 254 Fragments arranged by size: 919 289 254 212 197 193 10 1 1 EcoRI G'AATT_C Cuts at: 0 1027 1160 2076 Size: 1027 133 916 Fragments arranged by size: 1027 916 133 FspI TGC'GCA Cuts at: 0 616 2076 Size: 616 1460 HaeII r_GCGC'y Cuts at: 0 119 1783 1958 2076 Size: 119 1664 175 118 Fragments arranged by size: 1664 175 119 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 292 324 460 492 643 675 834 866 Size: 292 32 136 32 151 32 159 32 Cuts at: 866 997 1029 2035 2067 2076 Size: 131 32 1006 32 9 Fragments arranged by size: 1006 292 159 151 136 131 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 0 963 2076 Size: 963 1113 KasI G'GCGC_C Cuts at: 0 115 1954 2076 Size: 115 1839 122 Fragments arranged by size: 1839 122 115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 290 503 948 2076 Size: 290 213 445 1128 Fragments arranged by size: 1128 445 290 213 MscI TGG'CCA Cuts at: 0 79 1403 2076 Size: 79 1324 673 Fragments arranged by size: 1324 673 79 MslI CAynn'nnrTG Cuts at: 0 226 610 2076 Size: 226 384 1466 Fragments arranged by size: 1466 384 226 MspA1I CmG'CkG Cuts at: 0 83 640 1000 1488 1723 1799 1837 1869 Size: 83 557 360 488 235 76 38 32 Cuts at: 1869 2014 2020 2076 Size: 145 6 56 Fragments arranged by size: 557 488 360 235 145 83 76 56 38 32 6 NaeI GCC'GGC Cuts at: 0 724 1808 2076 Size: 724 1084 268 Fragments arranged by size: 1084 724 268 NarI GG'CG_CC Cuts at: 0 116 1955 2076 Size: 116 1839 121 Fragments arranged by size: 1839 121 116 NcoI C'CATG_G Cuts at: 0 752 2076 Size: 752 1324 NdeI CA'TA_TG Cuts at: 0 63 2076 Size: 63 2013 NgoMIV G'CCGG_C Cuts at: 0 722 1806 2076 Size: 722 1084 270 Fragments arranged by size: 1084 722 270 NspI r_CATG'y Cuts at: 0 783 1458 2076 Size: 783 675 618 Fragments arranged by size: 783 675 618 PasI CC'CwG_GG Cuts at: 0 54 2076 Size: 54 2022 PflMI CCAn_nnn'nTGG Cuts at: 0 297 668 753 2076 Size: 297 371 85 1323 Fragments arranged by size: 1323 371 297 85 PfoI T'CCnGG_A Cuts at: 0 584 1298 2076 Size: 584 714 778 Fragments arranged by size: 778 714 584 PpuMI rG'GwC_Cy Cuts at: 0 1629 2076 Size: 1629 447 PspOMI G'GGCC_C Cuts at: 0 212 709 2076 Size: 212 497 1367 Fragments arranged by size: 1367 497 212 PstI C_TGCA'G Cuts at: 0 1941 2076 Size: 1941 135 PvuII CAG'CTG Cuts at: 0 83 1000 1869 2014 2076 Size: 83 917 869 145 62 Fragments arranged by size: 917 869 145 83 62 SacI G_AGCT'C Cuts at: 0 373 2076 Size: 373 1703 SacII CC_GC'GG Cuts at: 0 1724 2021 2076 Size: 1724 297 55 Fragments arranged by size: 1724 297 55 SapI GCTCTTCn'nnn_ Cuts at: 0 1871 2076 Size: 1871 205 SfcI C'TryA_G Cuts at: 0 150 447 1937 2076 Size: 150 297 1490 139 Fragments arranged by size: 1490 297 150 139 SfiI GGCCn_nnn'nGGCC Cuts at: 0 184 2076 Size: 184 1892 SfoI GGC'GCC Cuts at: 0 117 1956 2076 Size: 117 1839 120 Fragments arranged by size: 1839 120 117 SmaI CCC'GGG Cuts at: 0 532 958 2076 Size: 532 426 1118 Fragments arranged by size: 1118 532 426 SmlI C'TyrA_G Cuts at: 0 1618 1856 2076 Size: 1618 238 220 Fragments arranged by size: 1618 238 220 SphI G_CATG'C Cuts at: 0 783 2076 Size: 783 1293 StyI C'CwwG_G Cuts at: 0 182 311 752 1919 2076 Size: 182 129 441 1167 157 Fragments arranged by size: 1167 441 182 157 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 67 679 2076 Size: 67 612 1397 Fragments arranged by size: 1397 612 67 TatI w'GTAC_w Cuts at: 0 506 2076 Size: 506 1570 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 59 91 1483 1515 2076 Size: 59 32 1392 32 561 Fragments arranged by size: 1392 561 59 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 61 1560 2076 Size: 61 1499 516 Fragments arranged by size: 1499 516 61 XmaI C'CCGG_G Cuts at: 0 530 956 2076 Size: 530 426 1120 Fragments arranged by size: 1120 530 426 XmnI GAAnn'nnTTC Cuts at: 0 919 2076 Size: 919 1157 ZraI GAC'GTC Cuts at: 0 1728 2076 Size: 1728 348 Enzymes that do cut: AarI AatII AcuI AhdI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfiI SfoI SmaI SmlI SphI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI