ALKBH8 ORF clone pFN21AE2046

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2046

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Clone info.

Species

human

Clone Name

pFN21AE2046 Sequence (with vector) Restriction Map

Product ID

FHC24446

ORF Sequence

DNA sequence (1992 bp)
Protein sequence (664 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590790]

Gene

KIEE2046

Symbol

ALKBH8

Alias : ABH8, MRT71, TRM9, TRMT9, TRMT9A

Description

alkB homolog 8, tRNA methyltransferase, transcript variant 2

Original Clone ID

cs02946

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1992 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 664 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8337.2 0 100.0 ALKBH8 CCDS73376.1 0 100.0 ALKBH8 CCDS47808.1 1.2e-27 49.0 KIAA1456 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR015095 1 37 PF09004 Alkylated DNA repair protein AlkB IPR005123 221 298 PF03171 Oxoglutarate/iron-dependent oxygenase IPR013216 412 501 PF08241 Methyltransferase type 11 ProfileScan IPR005123 220 337 PS51471 Oxoglutarate/iron-dependent oxygenase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_620130 664 613306 100.0 100.0 NP_001287939 667 613306 100.0 100.0 XP_016874043 614 613306 92.5 100.0 XP_016874042 617 613306 92.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2046 Download

>KIEE2046  1992 bp
ATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGG
AAACAGATTAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTAT
GCCACTCAGAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGGTGTGAGTCGGAACCAG
CTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGATGCTCTCTTAATGCCACCTAACAAG
CCGTACTCATTTGCAAGATACAGAACTACAGAAGAATCTAAGAGAGCCTATGTTACCCTC
AATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAGATCACTCTGTATTTGAATTTTGTG
GAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCAGGACTCATGGTAGTA
GAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGATTGGACAGAA
GATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGT
TATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTT
CCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATTAAACATAAA
CCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCTCATATTGAT
ACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAGATTGTCATG
GATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGGAGTTTGCTG
GTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGAAAATTTGAT
ACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTTGGAGACTTA
ACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGGCAAACACCT
TGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCCCCCTCATTT
CCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAGGTTTATGAA
GAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACATTGTGGAGTTT
TTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAATGGAAAGTAT
CTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAACCTTGTGGAC
ATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTACCAGTCCGC
AGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCAACAGCAGAG
CGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGGAAGGCACTC
ATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTATCTTAGAGGA
AACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGTGCAGAGGTCA
CTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTCCCCCGCATT
AATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAGCTGCCTGTT
CATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCACCTTAAGGGA
AATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAGGACCCAAGT
CCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGTGCCTGCAGG
ACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGGTGTGTGATT
CTTCAAAAGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE2046 Download

>KIEE2046  664 aa
MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQ
LLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFV
EKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFG
YEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQGIPAHID
THSAFEDEIVSLSLGSEIVMDFKHPDGIAVPVMLPRRSLLVMTGESRYLWTHGITCRKFD
TVQASESLKSGIITSDVGDLTLSKRGLRTSFTFRKVRQTPCNCSYPLVCDSQRKETPPSF
PESDKEASRLEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKY
LGINKELYMIGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAE
RRVAALQEIVRLLRPGGKALIYVWAMEQEYNKQKSKYLRGNRNSQGKKEEMNSDTSVQRS
LVEQMRDMGSRDSASSVPRINDSQEGGCNSRQVSNSKLPVHVNRTSFYSQDVLVPWHLKG
NPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEGACRTVSDVRILQSYYDQGNWCVI
LQKA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2046 Download

>pFN21AE2046  6697 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACAGCAACCATCAAAGTAATTACAAACTCAG
TAAAACTGAGAAGAAGTTCTTAAGGAAACAGATTAAAGCCAAGCATACTTTGCTGAGACA
TGAAGGCATTGAGACAGTATCCTATGCCACTCAGAGCCTGGTTGTTGCCAATGGTGGTTT
GGGTAATGGTGTGAGTCGGAACCAGCTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGA
TGCTCTCTTAATGCCACCTAACAAGCCGTACTCATTTGCAAGATACAGAACTACAGAAGA
ATCTAAGAGAGCCTATGTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAA
GATCACTCTGTATTTGAATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGC
CTTACCACCAGGACTCATGGTAGTAGAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCT
TTTGGAAAGTGTTGATTGGACAGAAGATACAGACAATCAAAACTCTCAAAAATCCTTAAA
ACACAGAAGAGTAAAGCATTTTGGTTATGAGTTCCACTATGAGAACAACAATGTAGATAA
AGATAAGCCATTATCTGGGGGTCTTCCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTT
GAGGAAAGGTTACATTAAACATAAACCTGATCAAATGACCATAAATCAGTATGAACCTGG
GCAAGGAATTCCCGCTCATATTGATACACATTCCGCTTTTGAGGATGAGATCGTTTCTCT
CAGTTTGGGGTCAGAGATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGT
TATGTTGCCTCGTCGGAGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACCCA
TGGAATCACGTGCAGAAAATTTGATACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAAT
TATCACCAGTGATGTTGGAGACTTAACTTTAAGCAAGAGGGGACTACGAACATCATTTAC
ATTTAGGAAAGTGAGGCAAACACCTTGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCA
GAGGAAAGAGACTCCCCCCTCATTTCCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCA
AGAGTACGTCCATCAGGTTTATGAAGAGATTGCTGGGCACTTCAGCAGCACAAGACATAC
CCCTTGGCCGCACATTGTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGA
TATTGGATGTGGTAATGGAAAGTATCTTGGCATCAATAAGGAGTTATATATGATTGGTTG
TGATCGTAGCCAAAACCTTGTGGACATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTG
TGATGCATTGGCAGTACCAGTCCGCAGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGT
TATTCATCATTTTGCAACAGCAGAGCGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACT
CCTGAGACCAGGTGGGAAGGCACTCATTTATGTCTGGGCAATGGAACAAGAATATAATAA
GCAGAAGTCCAAGTATCTTAGAGGAAACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAA
CAGTGATACCTCAGTGCAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGA
CTCGGCATCTTCTGTCCCCCGCATTAATGACTCTCAGGAAGGAGGATGTAATTCAAGGCA
AGTTTCTAATTCCAAGCTGCCTGTTCATGTTAACAGGACTTCTTTTTATTCTCAAGATGT
ACTGGTTCCCTGGCACCTTAAGGGAAATCCTGATAAAGGCAAACCTGTTGAGCCATTTGG
TCCCATAGGATCCCAGGACCCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTCCGTGA
GGGAGAACTGGAAGGTGCCTGCAGGACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTA
CGATCAAGGAAACTGGTGTGTGATTCTTCAAAAGGCCGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT