(Circular) (Six-base) MAPSORT of: pFN21AE2046.seq Check: 5923 from: 1 to: 6697 pFN21AE2046 6697 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4507 278 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4040 1937 Size: 2103 4594 Acc65I G'GTAC_C Cuts at: 4018 4018 Size: 6697 AclI AA'CG_TT Cuts at: 1563 4824 5197 1563 Size: 3261 373 3063 Fragments arranged by size: 3261 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3145 4760 6144 6412 1632 Size: 1513 1615 1384 268 1917 Fragments arranged by size: 1917 1615 1513 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2059 3797 820 Size: 197 1042 1738 3720 Fragments arranged by size: 3720 1738 1042 197 AflIII A'CryG_T Cuts at: 1203 5596 6573 1203 Size: 4393 977 1327 Fragments arranged by size: 4393 1327 977 AgeI A'CCGG_T Cuts at: 5528 5528 Size: 6697 AhdI GACnn_n'nnGTC Cuts at: 5426 5426 Size: 6697 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6697 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2828 1184 Size: 1644 5053 AlwNI CAG_nnn'CTG Cuts at: 2137 4440 6012 2137 Size: 2303 1572 2822 Fragments arranged by size: 2822 2303 1572 ApaLI G'TGCA_C Cuts at: 4754 5910 4754 Size: 1156 5541 ApoI r'AATT_y Cuts at: 1493 2356 2706 2897 3942 4006 4232 4384 6493 Size: 863 350 191 1045 64 226 152 2109 Cuts at: 6493 1493 Size: 1697 Fragments arranged by size: 2109 1697 1045 863 350 226 191 152 64 AseI AT'TA_AT Cuts at: 160 1032 3684 5251 160 Size: 872 2652 1567 1606 Fragments arranged by size: 2652 1606 1567 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 AvaI C'yCGr_G Cuts at: 1870 1943 4022 1870 Size: 73 2079 4545 Fragments arranged by size: 4545 2079 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3597 3630 3597 Size: 33 6664 BamHI G'GATC_C Cuts at: 3848 4027 4394 3848 Size: 179 367 6151 Fragments arranged by size: 6151 367 179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3792 3914 4018 5473 618 Size: 325 217 627 2005 122 104 1455 1842 Fragments arranged by size: 2005 1842 1455 627 325 217 122 104 BanII G_rGCy'C Cuts at: 1391 4016 1391 Size: 2625 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2594 928 Size: 971 695 5031 Fragments arranged by size: 5031 971 695 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4888 4922 1903 Size: 34 2951 34 3678 Fragments arranged by size: 3678 2951 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2128 4589 5799 2128 Size: 2461 1210 3026 Fragments arranged by size: 3026 2461 1210 BclI T'GATC_A Cuts at: 1548 2668 1548 Size: 1120 5577 BfrBI ATG'CAT Cuts at: 3365 3365 Size: 6697 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5308 136 Size: 107 122 71 631 605 3636 1525 Fragments arranged by size: 3636 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6692 6692 Size: 6697 BlpI GC'TnA_GC Cuts at: 4114 4114 Size: 6697 Bme1580I G_kGCm'C Cuts at: 1790 3159 4758 5914 1790 Size: 1369 1599 1156 2573 Fragments arranged by size: 2573 1599 1369 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6697 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4428 5386 442 Size: 416 53 83 399 24 3011 958 1753 Fragments arranged by size: 3011 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3134 5357 1239 Size: 1895 2223 2579 Fragments arranged by size: 2579 2223 1895 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2379 3756 4822 5687 5985 2379 Size: 1377 1066 865 298 3091 Fragments arranged by size: 3091 1377 1066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3478 5360 882 Size: 617 1979 1882 2219 Fragments arranged by size: 2219 1979 1882 617 BsaAI yAC'GTr Cuts at: 493 1204 2889 4488 493 Size: 711 1685 1599 2702 Fragments arranged by size: 2702 1685 1599 711 BsaBI GATnn'nnATC Cuts at: 6691 6691 Size: 6697 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4504 4886 5521 275 Size: 53 83 186 936 2971 382 635 1451 Fragments arranged by size: 2971 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5130 5528 5802 5949 6360 5130 Size: 398 274 147 411 5467 Fragments arranged by size: 5467 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2621 2651 3107 3137 1178 Size: 30 165 30 1218 30 456 30 4738 Fragments arranged by size: 4738 1218 456 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 2462 3605 1186 Size: 1273 3 1143 4278 Fragments arranged by size: 4278 1273 1143 3 BseYI C'CCAG_C Cuts at: 3152 5900 6396 3152 Size: 2748 496 3453 Fragments arranged by size: 3453 2748 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 2911 3635 1163 Size: 573 657 518 724 4225 Fragments arranged by size: 4225 724 657 573 518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4147 4908 5936 1540 Size: 275 186 2146 761 1028 2301 Fragments arranged by size: 2301 2146 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4758 4843 5914 4758 Size: 85 1071 5541 Fragments arranged by size: 5541 1071 85 BsmI GAATG_Cn' Cuts at: 4208 4301 4208 Size: 93 6604 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3159 4016 4758 4843 5914 1391 Size: 399 1369 857 742 85 1071 2174 Fragments arranged by size: 2174 1369 1071 857 742 399 85 BspEI T'CCGG_A Cuts at: 6360 6360 Size: 6697 BspHI T'CATG_A Cuts at: 4586 6316 4586 Size: 1730 4967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4052 844 Size: 3208 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2714 4584 1394 Size: 429 891 1870 3507 Fragments arranged by size: 3507 1870 891 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2805 3411 3525 5192 5366 65 Size: 1183 1557 606 114 1667 174 1396 Fragments arranged by size: 1667 1557 1396 1183 606 174 114 BsrFI r'CCGG_y Cuts at: 1700 1810 5341 5528 1700 Size: 110 3531 187 2869 Fragments arranged by size: 3531 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6697 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6697 BssSI C'ACGA_G Cuts at: 4757 5769 4757 Size: 1012 5685 BstAPI GCAn_nnn'nTGC Cuts at: 794 2089 794 Size: 1295 5402 BstBI TT'CG_AA Cuts at: 5501 5501 Size: 6697 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6697 BstYI r'GATC_y Cuts at: 1972 3848 4027 4394 4779 4796 6248 6334 6346 Size: 1876 179 367 385 17 1452 86 12 Cuts at: 6346 6692 1972 Size: 346 1977 Fragments arranged by size: 1977 1876 1452 385 367 346 179 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2878 513 Size: 552 939 874 4332 Fragments arranged by size: 4332 939 874 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5958 Fragments arranged by size: 5958 683 56 BtsI GCAGTG_nn' Cuts at: 2381 2817 3391 4213 5007 5027 2381 Size: 436 574 822 794 20 4051 Fragments arranged by size: 4051 822 794 574 436 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6662 DraI TTT'AAA Cuts at: 4001 4001 Size: 6697 DraIII CAC_nnn'GTG Cuts at: 3196 3196 Size: 6697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5704 809 Size: 507 4388 1802 Fragments arranged by size: 4388 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3185 4144 5033 Size: 54 1425 50 275 18 1355 959 889 Cuts at: 5033 6662 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1355 959 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4144 1537 Size: 275 2332 4090 Fragments arranged by size: 4090 2332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2521 3138 4627 1353 Size: 1168 617 1489 3423 Fragments arranged by size: 3423 1489 1168 617 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5284 5658 5804 387 Size: 1064 138 3695 374 146 1280 Fragments arranged by size: 3695 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3134 3145 4760 5357 6144 6412 1239 Size: 393 1502 11 1615 597 787 268 1524 Fragments arranged by size: 1615 1524 1502 787 597 393 268 11 EcoNI CCTnn'n_nnAGG Cuts at: 2700 3813 2700 Size: 1113 5584 EcoO109I rG'GnC_Cy Cuts at: 3856 3856 Size: 6697 EcoRI G'AATT_C Cuts at: 2706 3942 4006 4384 2706 Size: 1236 64 378 5019 Fragments arranged by size: 5019 1236 378 64 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2074 2074 Size: 6697 FspI TGC'GCA Cuts at: 5203 5203 Size: 6697 HaeII r_GCGC'y Cuts at: 1678 5844 1678 Size: 4166 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2325 2357 2668 Size: 32 135 32 530 32 191 32 311 Cuts at: 2668 2700 2740 2772 3650 3682 5338 5370 5412 Size: 32 40 32 878 32 1656 32 42 Cuts at: 5412 5444 1373 Size: 32 2626 Fragments arranged by size: 2626 1656 878 530 311 191 135 42 40 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3751 4041 4287 669 Size: 988 281 1813 290 246 3079 Fragments arranged by size: 3079 1813 988 290 281 246 HindIII A'AGCT_T Cuts at: 748 2620 748 Size: 1872 4825 HpaI GTT'AAC Cuts at: 3751 4287 3751 Size: 536 6161 KpnI G_GTAC'C Cuts at: 4022 4022 Size: 6697 MfeI C'AATT_G Cuts at: 4296 4296 Size: 6697 MluI A'CGCG_T Cuts at: 6573 6573 Size: 6697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2156 2813 2935 5811 5995 1305 Size: 851 657 122 2876 184 2007 Fragments arranged by size: 2876 2007 851 657 184 122 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4875 Fragments arranged by size: 4875 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2799 2810 4655 5014 5173 518 Size: 648 1633 11 1845 359 159 2042 Fragments arranged by size: 2042 1845 1633 648 359 159 11 MspA1I CmG'CkG Cuts at: 1606 2185 4113 4790 5938 6183 1606 Size: 579 1928 677 1148 245 2120 Fragments arranged by size: 2120 1928 1148 677 579 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2878 513 Size: 552 939 874 4332 Fragments arranged by size: 4332 939 874 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6697 NotI GC'GGCC_GC Cuts at: 4144 4144 Size: 6697 NsiI A_TGCA'T Cuts at: 3367 3367 Size: 6697 NspI r_CATG'y Cuts at: 1132 1229 4055 5600 1132 Size: 97 2826 1545 2229 Fragments arranged by size: 2826 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6697 PciI A'CATG_T Cuts at: 5596 5596 Size: 6697 PflMI CCAn_nnn'nTGG Cuts at: 1108 2594 1108 Size: 1486 5211 PmeI GTTT'AAAC Cuts at: 4001 4001 Size: 6697 PmlI CAC'GTG Cuts at: 2889 2889 Size: 6697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 2071 4766 4798 6279 6311 2039 Size: 32 2695 32 1481 32 2425 Fragments arranged by size: 2695 2425 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3856 3856 Size: 6697 PshAI GACnn'nnGTC Cuts at: 1613 3861 1613 Size: 2248 4449 PsiI TTA'TAA Cuts at: 4267 4267 Size: 6697 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2980 3012 2980 Size: 32 6665 PstI C_TGCA'G Cuts at: 830 3923 4049 830 Size: 3093 126 3478 Fragments arranged by size: 3478 3093 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6697 PvuII CAG'CTG Cuts at: 2185 2185 Size: 6697 SalI G'TCGA_C Cuts at: 1936 4039 1936 Size: 2103 4594 SbfI CC_TGCA'GG Cuts at: 3923 4049 3923 Size: 126 6571 ScaI AGT'ACT Cuts at: 4945 6657 4945 Size: 1712 4985 SexAI A'CCwGG_T Cuts at: 3487 3487 Size: 6697 SfcI C'TryA_G Cuts at: 826 1045 2271 3031 3919 4045 5180 5861 6052 Size: 219 1226 760 888 126 1135 681 191 Cuts at: 6052 826 Size: 1471 Fragments arranged by size: 1471 1226 1135 888 760 681 219 191 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6697 SmaI CCC'GGG Cuts at: 1872 4024 1872 Size: 2152 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2059 2394 3771 3797 4801 5702 Size: 197 926 116 335 1377 26 1004 901 Cuts at: 5702 5964 820 Size: 262 1553 Fragments arranged by size: 1553 1377 1004 926 901 335 262 197 116 26 SnaBI TAC'GTA Cuts at: 493 4488 493 Size: 3995 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6697 SphI G_CATG'C Cuts at: 1132 4055 1132 Size: 2923 3774 SspI AAT'ATT Cuts at: 5 52 4621 5 Size: 47 4569 2081 Fragments arranged by size: 4569 2081 47 StuI AGG'CCT Cuts at: 1517 2392 1517 Size: 875 5822 StyI C'CwwG_G Cuts at: 513 1065 2004 2878 3182 3577 4136 513 Size: 552 939 874 304 395 559 3074 Fragments arranged by size: 3074 939 874 559 552 395 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2811 4724 4741 4894 5079 5524 325 Size: 1255 1231 1913 17 153 185 445 1498 Fragments arranged by size: 1913 1498 1255 1231 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3778 4943 6655 Size: 274 80 33 51 1443 1801 1165 1712 Cuts at: 6655 96 Size: 138 Fragments arranged by size: 1801 1712 1443 1165 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3916 3948 6657 6689 3916 Size: 32 2709 32 3924 Fragments arranged by size: 3924 2709 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6697 XbaI T'CTAG_A Cuts at: 2861 4033 2861 Size: 1172 5525 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2155 2134 Size: 21 6676 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6697 XmaI C'CCGG_G Cuts at: 1870 4022 1870 Size: 2152 4545 XmnI GAAnn'nnTTC Cuts at: 2054 3471 4826 2054 Size: 1417 1355 3925 Fragments arranged by size: 3925 1417 1355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4505 276 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI