(Linear) (Six-base) MAPSORT of: KIEE2046.seq Check: 9102 from: 1 to: 1992 KIEE2046.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1140 1992 Size: 1140 852 AflII C'TTAA_G Cuts at: 0 54 1792 1992 Size: 54 1738 200 Fragments arranged by size: 1738 200 54 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 823 1992 Size: 823 1169 AlwNI CAG_nnn'CTG Cuts at: 0 132 1992 Size: 132 1860 ApoI r'AATT_y Cuts at: 0 351 701 892 1937 1992 Size: 351 350 191 1045 55 Fragments arranged by size: 1045 351 350 191 55 AseI AT'TA_AT Cuts at: 0 1679 1992 Size: 1679 313 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1592 1625 1992 Size: 1592 33 367 Fragments arranged by size: 1592 367 33 BamHI G'GATC_C Cuts at: 0 1843 1992 Size: 1843 149 BanI G'GyrC_C Cuts at: 0 1787 1909 1992 Size: 1787 122 83 Fragments arranged by size: 1787 122 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 589 1992 Size: 589 1403 BciVI GTATCCnnnnn_n' Cuts at: 0 123 1992 Size: 123 1869 BclI T'GATC_A Cuts at: 0 663 1992 Size: 663 1329 BfrBI ATG'CAT Cuts at: 0 1360 1992 Size: 1360 632 Bme1580I G_kGCm'C Cuts at: 0 1154 1992 Size: 1154 838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1129 1992 Size: 1129 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 1751 1992 Size: 374 1377 241 Fragments arranged by size: 1377 374 241 BsaI GGTCTCn'nnnn_ Cuts at: 0 1473 1992 Size: 1473 519 BsaAI yAC'GTr Cuts at: 0 884 1992 Size: 884 1108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 616 646 1102 1132 1992 Size: 616 30 456 30 860 Fragments arranged by size: 860 616 456 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 454 457 1600 1992 Size: 454 3 1143 392 Fragments arranged by size: 1143 454 392 3 BseYI C'CCAG_C Cuts at: 0 1147 1992 Size: 1147 845 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 906 1630 1992 Size: 388 518 724 362 Fragments arranged by size: 724 518 388 362 Bsp1286I G_dGCh'C Cuts at: 0 1154 1992 Size: 1154 838 BsrBI CCG'CTC Cuts at: 0 709 1992 Size: 709 1283 BsrDI GCAATG_nn' Cuts at: 0 800 1406 1520 1992 Size: 800 606 114 472 Fragments arranged by size: 800 606 472 114 BstAPI GCAn_nnn'nTGC Cuts at: 0 84 1992 Size: 84 1908 BstYI r'GATC_y Cuts at: 0 1843 1992 Size: 1843 149 BtgI C'CryG_G Cuts at: 0 873 1992 Size: 873 1119 BtsI GCAGTG_nn' Cuts at: 0 376 812 1386 1992 Size: 376 436 574 606 Fragments arranged by size: 606 574 436 376 DraIII CAC_nnn'GTG Cuts at: 0 1191 1992 Size: 1191 801 EaeI y'GGCC_r Cuts at: 0 1180 1992 Size: 1180 812 EarI CTCTTCn'nnn_ Cuts at: 0 516 1133 1992 Size: 516 617 859 Fragments arranged by size: 859 617 516 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1129 1140 1992 Size: 1129 11 852 Fragments arranged by size: 1129 852 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 695 1808 1992 Size: 695 1113 184 Fragments arranged by size: 1113 695 184 EcoO109I rG'GnC_Cy Cuts at: 0 1851 1992 Size: 1851 141 EcoRI G'AATT_C Cuts at: 0 701 1937 1992 Size: 701 1236 55 Fragments arranged by size: 1236 701 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 69 1992 Size: 69 1923 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 320 352 663 695 735 767 Size: 97 32 191 32 311 32 40 32 Cuts at: 767 1645 1677 1992 Size: 878 32 315 Fragments arranged by size: 878 315 311 191 97 40 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1746 1992 Size: 1746 246 HindIII A'AGCT_T Cuts at: 0 615 1992 Size: 615 1377 HpaI GTT'AAC Cuts at: 0 1746 1992 Size: 1746 246 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 151 808 930 1992 Size: 151 657 122 1062 Fragments arranged by size: 1062 657 151 122 MslI CAynn'nnrTG Cuts at: 0 794 805 1992 Size: 794 11 1187 Fragments arranged by size: 1187 794 11 MspA1I CmG'CkG Cuts at: 0 180 1992 Size: 180 1812 NcoI C'CATG_G Cuts at: 0 873 1992 Size: 873 1119 NsiI A_TGCA'T Cuts at: 0 1362 1992 Size: 1362 630 PflMI CCAn_nnn'nTGG Cuts at: 0 589 1992 Size: 589 1403 PmlI CAC'GTG Cuts at: 0 884 1992 Size: 884 1108 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 1992 Size: 34 32 1926 Fragments arranged by size: 1926 34 32 PpuMI rG'GwC_Cy Cuts at: 0 1851 1992 Size: 1851 141 PshAI GACnn'nnGTC Cuts at: 0 1856 1992 Size: 1856 136 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 975 1007 1992 Size: 975 32 985 Fragments arranged by size: 985 975 32 PstI C_TGCA'G Cuts at: 0 1918 1992 Size: 1918 74 PvuII CAG'CTG Cuts at: 0 180 1992 Size: 180 1812 SbfI CC_TGCA'GG Cuts at: 0 1918 1992 Size: 1918 74 SexAI A'CCwGG_T Cuts at: 0 1482 1992 Size: 1482 510 SfcI C'TryA_G Cuts at: 0 266 1026 1914 1992 Size: 266 760 888 78 Fragments arranged by size: 888 760 266 78 SmlI C'TyrA_G Cuts at: 0 54 389 1766 1792 1992 Size: 54 335 1377 26 200 Fragments arranged by size: 1377 335 200 54 26 StuI AGG'CCT Cuts at: 0 387 1992 Size: 387 1605 StyI C'CwwG_G Cuts at: 0 873 1177 1572 1992 Size: 873 304 395 420 Fragments arranged by size: 873 420 395 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 806 1992 Size: 806 1186 TatI w'GTAC_w Cuts at: 0 1773 1992 Size: 1773 219 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1911 1943 1992 Size: 1911 32 49 Fragments arranged by size: 1911 49 32 XbaI T'CTAG_A Cuts at: 0 856 1992 Size: 856 1136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 129 150 1992 Size: 129 21 1842 Fragments arranged by size: 1842 129 21 XmnI GAAnn'nnTTC Cuts at: 0 49 1466 1992 Size: 49 1417 526 Fragments arranged by size: 1417 526 49 Enzymes that do cut: AcuI AflII AlfI AlwNI ApoI AseI BaeI BamHI BanI BbsI BciVI BclI BfrBI Bme1580I BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI Bsp1286I BsrBI BsrDI BstAPI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI MmeI MslI MspA1I NcoI NsiI PflMI PmlI PpiI PpuMI PshAI PsrI PstI PvuII SbfI SexAI SfcI SmlI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BanII BbeI BbvCI BcgI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XhoI XmaI ZraI