Restriction map for
pFN21AE2046
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanII
BbeI BbvCI BcgI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AlwNI 127 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1678 ATTAAT AT^TAAT
AsnI 1678 ATTAAT AT^TAAT
BamHI 1843 GGATCC G^GATCC
BbrPI 882 CACGTG CAC^GTG
BbsI 596 GTCTTC GAAGAC(2/6)
BciVI 112 GTATCC GTATCC(6/5)
BclI 663 TGATCA T^GATCA
BcnI 186 CCCGG CC^[GC]GG
BfaI 857 CTAG C^TAG
BmyI 1150 GGGCAC G[AGT]GC[ACT]^C
BpmI 1108 CTGGAG CTGGAG(16/14)
BpuAI 596 GTCTTC GAAGAC(2/6)
BsaAI 882 CACGTG [CT]AC^GT[GA]
BsaI 1479 GAGACC GGTCTC(1/5)
Bsp1286I 1150 GGGCAC G[AGT]GC[ACT]^C
BsrBI 707 CCGCTC CCGCTC(-3/-3)
BstAPI 78 GCATACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1843 GGATCC [GA]^GATC[CT]
DraII 1850 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1186 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 873 CCATGG C^C[GA][CT]GG
EaeI 1180 TGGCCG [CT]^GGCC[GA]
EcoO109I 1850 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1358 ATGCAT ATGCA^T
FbaI 663 TGATCA T^GATCA
HapII 187 CCGG C^CGG
HincII 1744 GTTAAC GT[CT]^[GA]AC
HindII 1744 GTTAAC GT[CT]^[GA]AC
HindIII 615 AAGCTT A^AGCTT
HpaI 1744 GTTAAC GTT^AAC
HpaII 187 CCGG C^CGG
MaeI 857 CTAG C^TAG
MflI 1843 GGATCC [GA]^GATC[CT]
MspA1I 178 CAGCTG C[AC]G^C[GT]G
MspI 187 CCGG C^CGG
MwoI 78 GCATACTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 186 CCCGG CC^[GC]GG
NcoI 873 CCATGG C^CATGG
NsiI 1358 ATGCAT ATGCA^T
PflMI 583 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 882 CACGTG CAC^GTG
PmlI 882 CACGTG CAC^GTG
PpuMI 1850 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1852 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1678 ATTAAT AT^TAAT
PstI 1914 CTGCAG CTGCA^G
PvuII 178 CAGCTG CAG^CTG
SbfI 1913 CCTGCAGG CCTGCA^GG
SexAI 1482 ACCAGGT A^CC[AT]GGT
Sse8387I 1913 CCTGCAGG CCTGCA^GG
StuI 385 AGGCCT AGG^CCT
Van91I 583 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 856 TCTAGA T^CTAGA
XhoII 1843 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 54 CTTAAG C^TTAAG
1792 CTTAAG
AlwI 1844 GATCC GGATC(4/5)
1843 GGATC
Asp700I 45 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1462 GAAATTGTTC
BanI 1787 GGCACC G^G[CT][GA]CC
1909 GGTGCC
BfrI 54 CTTAAG C^TTAAG
1792 CTTAAG
Cac8I 1396 GCCTGC GC[ACGT]^[ACGT]GC
1912 GCCTGC
EarI 521 GAAGAG CTCTTC(1/4)
1138 GAAGAG
EcoNI 691 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1804 CCTGATAAAGG
EcoRI 701 GAATTC G^AATTC
1937 GAATTC
FauI 706 CCCGC CCCGC(4/6)
1673 CCCGC
HphI 840 GGTGA GGTGA(8/7)
938 TCACC
Ksp632I 521 GAAGAG CTCTTC(1/4)
1138 GAAGAG
MaeII 883 ACGT A^CGT
1121 ACGT
MslI 790 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
801 CATTGCAGTG
TaqI 1470 TCGA T^CGA
1650 TCGA
Tsp45I 1617 GTCAC ^GT[GC]AC
1637 GTGAC
TthHB8I 1470 TCGA T^CGA
1650 TCGA
XcmI 122 CCACTCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
143 CCAATGGTGGTTTGG
XmnI 45 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1462 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsrDI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI