NCF2 ORF clone pFN21AE1377

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Halotag ORF Clone pFN21AE1377

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Clone info.

Species

human

Clone Name

pFN21AE1377 Sequence (with vector) Restriction Map

Product ID

FHC08013

ORF Sequence

DNA sequence (1578 bp)
Protein sequence (526 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590135]

Gene

KIEE1377

Symbol

NCF2

Alias : NCF-2, NOXA2, P67-PHOX, P67PHOX

Description

neutrophil cytosolic factor 2, transcript variant 1

Original Clone ID

oc01377

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1578 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 526 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1356.1 1.8e-136 99.6 NCF2 CCDS53447.1 5.6e-92 91.1 NCF2 CCDS53446.1 1.1e-82 84.4 NCF2 CCDS7042.1 9.8e-16 31.4 NOXA1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000108 218 235 PR00499 Neutrophil cytosol factor 2 p67phox IPR000108 258 277 PR00499 Neutrophil cytosol factor 2 p67phox IPR000108 284 303 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 460 470 PR00452 Src homology-3 domain IPR000108 462 482 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 474 489 PR00452 Src homology-3 domain IPR000108 482 498 PR00499 Neutrophil cytosol factor 2 p67phox IPR000108 498 511 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 502 514 PR00452 Src homology-3 domain HMMPfam IPR001440 71 98 PF00515 Tetratricopeptide TPR-1 IPR001452 246 291 PF00018 Src homology-3 domain IPR000270 352 427 PF00564 Phox/Bem1p IPR001452 464 507 PF00018 Src homology-3 domain HMMSmart IPR019734 37 70 SM00028 Tetratricopeptide repeat IPR019734 71 104 SM00028 Tetratricopeptide repeat IPR019734 121 154 SM00028 Tetratricopeptide repeat IPR001452 243 298 SM00326 Src homology-3 domain IPR000270 351 429 SM00666 Phox/Bem1p IPR001452 460 515 SM00326 Src homology-3 domain ProfileScan IPR019734 37 70 PS50005 Tetratricopeptide repeat IPR013026 37 104 PS50293 Tetratricopeptide repeat-containing IPR019734 71 104 PS50005 Tetratricopeptide repeat IPR019734 121 154 PS50005 Tetratricopeptide repeat IPR001452 240 299 PS50002 Src homology-3 domain IPR001452 457 516 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011507882 526 233710,608515 99.6 100.0 NP_000424 526 233710,608515 99.6 100.0 NP_001121123 526 233710,608515 99.6 100.0 XP_011507883 526 233710,608515 99.6 100.0 NP_001177723 481 233710,608515 91.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1377 Download

>KIEE1377  1578 bp
ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAG
GACTGGAAGGGAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGC
TTCAACATTGGCTGCATGTACACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTT
ACCAGAAGCATTAACCGAGACAAGCACTTGGCAGTGGCTTACTTCCAACGAGGGATGCTC
TACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACCTTAAAGAAGCCTTGATTCAG
CTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAAGCTGTTTGCC
TGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAATGGAAAAAAGCT
GAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCAAAATCGAC
AAGGCGATGGAGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGC
AGGCTGTTTCGACCAAATGAGAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGC
AAGGCAACGGTCGTGGCATCTGTGGTGGATCAAGACAGTTTCTCTGGGTTTGCCCCTCTG
CAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCCCAGAGATCTTCAGGGCTCTG
GAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAAGAAGAGCTCCAG
GTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCACGGTC
ATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGG
ATCCACCCTCAGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCT
CCTAGTTCCAAAGCCCCTGGAAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAG
CCTAAGGAAGTGAAGCTCAGTGTTCCCATGCCCTACACACTCAAGGTGCACTACAAGTAC
ACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCCAGGTCCGGGACATGGTGTCT
AAGAAACTGGAGCTCCGGCTGGAACAAACTAAGCTGAGCTATCGGCCTCGGGACAGCAAT
GAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAAACTAC
TGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAG
GAAAGTGAAAAAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGC
CAAGTGGAGGCACTCTTCAGTTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAA
GGGGATATAATCCTGGTGTTATCAAAGGTGAATGAAGAATGGCTGGAAGGGGAGTGCAAA
GGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACTGCGCAACTACAGATTTGGAA
AGCACTCGGAGAGAAGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE1377 Download

>KIEE1377  526 aa
MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAF
TRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFA
CEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVG
RLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRAL
EGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELR
IHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKY
TVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSNELVPLSEDSMKDAWGQVKNY
CLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQE
GDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1377 Download

>pFN21AE1377  6283 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGA
AGGGGTGCTGGCAGCGGACAAGAAGGACTGGAAGGGAGCCCTGGATGCCTTCAGTGCCGT
CCAGGACCCCCACTCCCGGATTTGCTTCAACATTGGCTGCATGTACACTATCCTGAAGAA
CATGACTGAAGCAGAGAAGGCCTTTACCAGAAGCATTAACCGAGACAAGCACTTGGCAGT
GGCTTACTTCCAACGAGGGATGCTCTACTACCAGACAGAGAAATATGATTTGGCTATCAA
AGACCTTAAAGAAGCCTTGATTCAGCTTCGAGGGAACCAGCTGATAGACTATAAGATCCT
GGGGCTCCAGTTCAAGCTGTTTGCCTGTGAGGTGTTATATAACATTGCTTTCATGTATGC
CAAGAAGGAGGAATGGAAAAAAGCTGAAGAACAGTTAGCATTGGCCACGAGCATGAAGTC
TGAGCCCAGACATTCCAAAATCGACAAGGCGATGGAGTGTGTCTGGAAGCAGAAGCTATA
TGAGCCAGTGGTGATCCCTGTGGGCAGGCTGTTTCGACCAAATGAGAGACAAGTGGCTCA
GCTGGCCAAGAAGGATTACCTAGGCAAGGCAACGGTCGTGGCATCTGTGGTGGATCAAGA
CAGTTTCTCTGGGTTTGCCCCTCTGCAACCACAGGCAGCTGAGCCTCCACCCAGACCGAA
AACCCCAGAGATCTTCAGGGCTCTGGAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGT
GCCTGAGACAAAAGAAGAGCTCCAGGTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAA
GGGCAATGATAACTGGGCCACGGTCATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAA
CTACCTTGAACCAGTTGAGCTGCGGATCCACCCTCAGCAGCAGCCCCAGGAGGAAAGCTC
TCCGCAGTCCGACATCCCAGCTCCTCCTAGTTCCAAAGCCCCTGGAAGACCCCAGCTGTC
ACCAGGCCAGAAACAAAAAGAAGAGCCTAAGGAAGTGAAGCTCAGTGTTCCCATGCCCTA
CACACTCAAGGTGCACTACAAGTACACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTA
CAGCCAGGTCCGGGACATGGTGTCTAAGAAACTGGAGCTCCGGCTGGAACAAACTAAGCT
GAGCTATCGGCCTCGGGACAGCAATGAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGA
TGCCTGGGGCCAGGTGAAAAACTACTGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGA
CCAAGGCTTTCCAGATGAACCCAAGGAAAGTGAAAAAGCTGATGCTAATAACCAGACAAC
AGAACCTCAGCTTAAGAAAGGCAGCCAAGTGGAGGCACTCTTCAGTTATGAGGCTACCCA
ACCAGAGGACCTGGAGTTTCAGGAAGGGGATATAATCCTGGTGTTATCAAAGGTGAATGA
AGAATGGCTGGAAGGGGAGTGCAAAGGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGA
AGACTGCGCAACTACAGATTTGGAAAGCACTCGGAGAGAAGTCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT