################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI BbeI BbrPI BcgI BciVI BclI BglI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflII 1366 CTTAAG C^TTAAG AhdI 1129 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1066 GTGCAC G^TGCAC AlwNI 21 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1021 CCTAAGG CC^T[ACGT]AGG ApaLI 1066 GTGCAC G^TGCAC AspEI 1129 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1540 TGCGCA TGC^GCA AvrII 594 CCTAGG C^CTAGG BamHI 899 GGATCC G^GATCC BanI 1206 GGTGCC G^G[CT][GA]CC BfrI 1366 CTTAAG C^TTAAG BglII 704 AGATCT A^GATCT BlnI 594 CCTAGG C^CTAGG BseRI 956 CTCCTC GAGGAG(10/8) BsiEI 608 CGGTCG CG[GA][CT]^CG Bsp1407I 137 TGTACA T^GTACA BspHI 1088 TCATGA T^CATGA BsrGI 137 TGTACA T^GTACA BssSI 441 CACGAG CACGAG(-5/-1) BstEII 1291 GGTGACC G^GT[ACGT]ACC BstPI 1291 GGTGACC G^GT[ACGT]ACC Bsu36I 1021 CCTAAGG CC^T[ACGT]AGG CfoI 1541 GCGC GCG^C DraIII 733 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 833 CCACGG C^C[GA][CT]GG Eam1105I 1129 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1021 CCTAAGG CC^T[ACGT]AGG EcoO65I 1291 GGTGACC G^GT[ACGT]ACC FspI 1540 TGCGCA TGC^GCA HhaI 1541 GCGC GCG^C HinP1I 1541 GCGC G^CGC NspI 134 GCATGT [GA]CATG^[CT] RcaI 1088 TCATGA T^CATGA SmaI 1102 CCCGGG CCC^GGG SspBI 137 TGTACA T^GTACA StuI 173 AGGCCT AGG^CCT TfiI 294 GATTC G^A[AT]TC XmaCI 1102 CCCGGG C^CCGGG XmaI 1102 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 138 GTAC GT^AC 1077 GTAC AvaI 1102 CCCGGG C^[CT]CG[GA]G 1187 CTCGGG BalI 437 TGGCCA TGG^CCA 578 TGGCCA BbvCI 907 CCTCAGC CCTCAGC(-5/-2) 1360 CCTCAGC BfaI 595 CTAG C^TAG 962 CTAG BsoBI 1102 CCCGGG C^[CT]CG[GA]G 1187 CTCGGG DraII 98 AGGACCC [GA]G^G[ACGT]CC[CT] 1421 AGGACCT EaeI 437 TGGCCA [CT]^GGCC[GA] 578 TGGCCA EcoNI 6 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 594 CCTAGGCAAGG EcoO109I 98 AGGACCC [GA]G^G[ACGT]CC[CT] 1421 AGGACCT MaeI 595 CTAG C^TAG 962 CTAG MaeIII 993 GTCAC ^GT[ACGT]AC 1292 GTGAC MluNI 437 TGGCCA TGG^CCA 578 TGGCCA MscI 437 TGGCCA TGG^CCA 578 TGGCCA MslI 441 CACGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CACGGTCATG PleI 1095 GACTC GAGTC(4/5) 1266 GACTC PpuMI 98 AGGACCC [GA]G^G[AT]CC[CT] 1421 AGGACCT RsaI 138 GTAC GT^AC 1077 GTAC SacI 772 GAGCTC GAGCT^C 1150 GAGCTC SapI 769 GAAGAGC GCTCTTC(1/4) 1015 GAAGAGC SfcI 1113 CTACAG C^T[GA][CT]AG 1547 CTACAG SmlI 1060 CTCAAG C^T[CT][GA]AG 1366 CTTAAG Tsp45I 993 GTCAC ^GT[GC]AC 1292 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BanII BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI TspRI TthHB8I XhoII