ILKAP ORF clone pFN21AE1338

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Halotag ORF Clone pFN21AE1338

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Clone info.

Species

human

Clone Name

pFN21AE1338 Sequence (with vector) Restriction Map

Product ID

FHC09890

ORF Sequence

DNA sequence (1176 bp)
Protein sequence (392 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590373]

Gene

KIEE1338

Symbol

ILKAP

Alias : ILKAP2, ILKAP3, PP2C-DELTA, PP2CD, PPM1O

Description

integrin-linked kinase-associated serine/threonine phosphatase

Original Clone ID

oc01338

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1176 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 392 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2526.1 5.8e-141 100.0 ILKAP CCDS46271.1 3.1e-16 32.2 PPM1B CCDS1816.1 3.2e-16 32.2 PPM1B CCDS1817.1 3.8e-16 32.2 PPM1B CCDS33886.1 2.4e-15 31.9 PPM1L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001932 113 383 PF00481 Protein phosphatase 2C-like HMMSmart IPR001932 94 388 SM00332 Protein phosphatase 2C-like Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1338 Download

>KIEE1338  1176 bp
ATGGACCTCTTCGGGGACCTGCCGGAGCCCGAGCGCTCGCCGCGCCCGGCTGCCGGGAAA
GAAGCTCAGAAAGGACCCCTGCTCTTTGATGACCTCCCTCCGGCCAGCAGTACTGACTCA
GGATCAGGGGGACCTTTGCTTTTTGATGATCTCCCACCCGCTAGCAGTGGCGATTCAGGT
TCTCTTGCCACATCAATATCCCAGATGGTAAAGACTGAAGGGAAAGGAGCAAAGAGAAAA
ACCTCCGAGGAAGAGAAGAATGGCAGTGAAGAGCTTGTGGAAAAGAAAGTTTGTAAAGCC
TCTTCGGTGATCTTTGGTCTGAAGGGCTATGTGGCTGAGCGGAAGGGTGAGAGGGAGGAG
ATGCAGGATGCCCACGTCATCCTGAACGACATCACCGAGGAGTGTAGGCCCCCATCGTCC
CTCATTACTCGGGTTTCATATTTTGCTGTTTTTGATGGACATGGAGGAATTCGAGCCTCA
AAATTTGCTGCACAGAATTTGCATCAAAACTTAATCAGAAAATTTCCTAAAGGAGATGTA
ATCAGTGTAGAGAAAACCGTGAAGAGATGCCTTTTGGACACTTTCAAGCATACTGATGAA
GAGTTCCTTAAACAAGCTTCCAGCCAGAAGCCTGCCTGGAAAGATGGGTCCACTGCCACG
TGTGTTCTGGCTGTAGACAACATTCTTTATATTGCCAACCTCGGAGATAGTCGGGCAATC
TTGTGTCGTTATAATGAGGAGAGTCAAAAACATGCAGCCTTAAGCCTCAGCAAAGAGCAT
AATCCAACTCAGTATGAAGAGCGGATGAGGATACAGAAGGCTGGAGGAAACGTCAGGGAT
GGGCGTGTTTTGGGCGTGCTAGAGGTGTCACGCTCCATTGGGGACGGGCAGTACAAGCGC
TGCGGTGTCACCTCTGTGCCCGACATCAGACGCTGCCAGCTGACCCCCAATGACAGGTTC
ATTTTGTTGGCCTGTGATGGGCTCTTCAAGGTCTTTACCCCAGAAGAAGCCGTGAACTTC
ATCTTGTCCTGTCTCGAGGATGAAAAGATCCAGACCCGGGAAGGGAAGTCCGCAGCCGAC
GCCCGCTACGAAGCAGCCTGCAACAGGCTGGCCAACAAGGCGGTGCAGCGGGGCTCGGCC
GACAACGTCACTGTGATGGTGGTGCGGATAGGGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE1338 Download

>KIEE1338  392 aa
MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSG
SLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREE
MQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDV
ISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAI
LCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKR
CGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAAD
ARYEAACNRLANKAVQRGSADNVTVMVVRIGH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1338 Download

>pFN21AE1338  5881 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCTCTTCGGGGACCTGCCGGAGCCCGAGCG
CTCGCCGCGCCCGGCTGCCGGGAAAGAAGCTCAGAAAGGACCCCTGCTCTTTGATGACCT
CCCTCCGGCCAGCAGTACTGACTCAGGATCAGGGGGACCTTTGCTTTTTGATGATCTCCC
ACCCGCTAGCAGTGGCGATTCAGGTTCTCTTGCCACATCAATATCCCAGATGGTAAAGAC
TGAAGGGAAAGGAGCAAAGAGAAAAACCTCCGAGGAAGAGAAGAATGGCAGTGAAGAGCT
TGTGGAAAAGAAAGTTTGTAAAGCCTCTTCGGTGATCTTTGGTCTGAAGGGCTATGTGGC
TGAGCGGAAGGGTGAGAGGGAGGAGATGCAGGATGCCCACGTCATCCTGAACGACATCAC
CGAGGAGTGTAGGCCCCCATCGTCCCTCATTACTCGGGTTTCATATTTTGCTGTTTTTGA
TGGACATGGAGGAATTCGAGCCTCAAAATTTGCTGCACAGAATTTGCATCAAAACTTAAT
CAGAAAATTTCCTAAAGGAGATGTAATCAGTGTAGAGAAAACCGTGAAGAGATGCCTTTT
GGACACTTTCAAGCATACTGATGAAGAGTTCCTTAAACAAGCTTCCAGCCAGAAGCCTGC
CTGGAAAGATGGGTCCACTGCCACGTGTGTTCTGGCTGTAGACAACATTCTTTATATTGC
CAACCTCGGAGATAGTCGGGCAATCTTGTGTCGTTATAATGAGGAGAGTCAAAAACATGC
AGCCTTAAGCCTCAGCAAAGAGCATAATCCAACTCAGTATGAAGAGCGGATGAGGATACA
GAAGGCTGGAGGAAACGTCAGGGATGGGCGTGTTTTGGGCGTGCTAGAGGTGTCACGCTC
CATTGGGGACGGGCAGTACAAGCGCTGCGGTGTCACCTCTGTGCCCGACATCAGACGCTG
CCAGCTGACCCCCAATGACAGGTTCATTTTGTTGGCCTGTGATGGGCTCTTCAAGGTCTT
TACCCCAGAAGAAGCCGTGAACTTCATCTTGTCCTGTCTCGAGGATGAAAAGATCCAGAC
CCGGGAAGGGAAGTCCGCAGCCGACGCCCGCTACGAAGCAGCCTGCAACAGGCTGGCCAA
CAAGGCGGTGCAGCGGGGCTCGGCCGACAACGTCACTGTGATGGTGGTGCGGATAGGGCA
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T