Restriction map for
pFN21AE1338
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BanI BbeI BbsI BcgI BclI
BglII BlnI BpuAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco81I EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 673 GTAGAC GT^[AC][GT]AC
AccII 41 CGCG CG^CG
AcyI 1078 GACGCC G[GA]^CG[CT]C
AflII 759 CTTAAG C^TTAAG
AflIII 658 ACGTGT A^C[GA][CT]GT
AlwNI 927 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1141 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1109 TGGCCA TGG^CCA
BbrPI 657 CACGTG CAC^GTG
BbvCI 765 CCTCAGC CCTCAGC(-5/-2)
BciVI 809 GGATAC GTATCC(6/5)
BfrI 759 CTTAAG C^TTAAG
BglI 1111 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 334 GCTGAGC GC^T[ACGT]AGC
BpmI 821 CTGGAG CTGGAG(16/14)
Bpu1102I 334 GCTGAGC GC^T[ACGT]AGC
BsaAI 657 CACGTG [CT]AC^GT[GA]
BsaHI 1078 GACGCC G[GA]^CG[CT]C
BsiEI 1136 CGGCCG CG[GA][CT]^CG
BsmAI 1031 GTCTC GTCTC(1/5)
BspMI 17 ACCTGC ACCTGC(4/8)
BstNI 635 CCTGG CC^[AT]GG
BstUI 41 CGCG CG^CG
BstYI 1046 AGATCC [GA]^GATC[CT]
CelII 334 GCTGAGC GC^T[ACGT]AGC
EagI 1136 CGGCCG C^GGCCG
EclXI 1136 CGGCCG C^GGCCG
Eco52I 1136 CGGCCG C^GGCCG
EcoNI 1097 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 467 GAATTC G^AATTC
EcoRII 635 CCTGG ^CC[AT]GG
Hin1I 1078 GACGCC G[GA]^CG[CT]C
HindIII 614 AAGCTT A^AGCTT
MflI 1046 AGATCC [GA]^GATC[CT]
MluNI 1109 TGGCCA TGG^CCA
MscI 1109 TGGCCA TGG^CCA
MvaI 635 CCTGG CC^[AT]GG
MvnI 41 CGCG CG^CG
NheI 160 GCTAGC G^CTAGC
NspI 750 ACATGC [GA]CATG^[CT]
PaeR7I 1033 CTCGAG C^TCGAG
PflFI 1141 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 657 CACGTG CAC^GTG
PmlI 657 CACGTG CAC^GTG
PvuII 937 CAGCTG CAG^CTG
ScaI 109 AGTACT AGT^ACT
SfcI 671 CTGTAG C^T[GA][CT]AG
SmaI 1055 CCCGGG CCC^GGG
TfiI 172 GATTC G^A[AT]TC
Tth111I 1141 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 656 CCACGTGTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1033 CTCGAG C^TCGAG
XhoII 1046 AGATCC [GA]^GATC[CT]
XmaCI 1055 CCCGGG C^CCGGG
XmaI 1055 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 110 GTAC GT^AC
891 GTAC
AlwI 1047 GATCC GGATC(4/5)
121 GGATC
Aor51HI 32 AGCGCT AGC^GCT
896 AGCGCT
BfaI 161 CTAG C^TAG
859 CTAG
BsgI 488 CTGCAC GTGCAG(16/14)
1123 GTGCAG
BsrBI 337 GAGCGG CCGCTC(-3/-3)
799 GAGCGG
Eco47III 32 AGCGCT AGC^GCT
896 AGCGCT
HaeII 32 AGCGCT [GA]GCGC^[CT]
896 AGCGCT
HgaI 929 GACGC GACGC(5/10)
1078 GACGC
MaeI 161 CTAG C^TAG
859 CTAG
MspA1I 937 CAGCTG C[AC]G^C[GT]G
1126 CAGCGG
NlaIII 460 CATG CATG^
751 CATG
PleI 115 GACTC GAGTC(4/5)
741 GAGTC
RsaI 110 GTAC GT^AC
891 GTAC
SmlI 759 CTTAAG C^T[CT][GA]AG
1033 CTCGAG
TaqI 471 TCGA T^CGA
1034 TCGA
TthHB8I 471 TCGA T^CGA
1034 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MwoI NciI
NdeII NlaIV PpuMI SapI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp45I Tsp509I TspRI