Restriction map for
pFN21AE1338
(Linear) (Six-base) MAPSORT of: KIEE1338.seq Check: 7639 from: 1 to: 1176
KIEE1338.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AccI GT'mk_AC
Cuts at: 0 674 1176
Size: 674 502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 340 1176
Size: 236 104 836
Fragments arranged by size:
836 236 104
AfeI AGC'GCT
Cuts at: 0 34 898 1176
Size: 34 864 278
Fragments arranged by size:
864 278 34
AflII C'TTAA_G
Cuts at: 0 759 1176
Size: 759 417
AflIII A'CryG_T
Cuts at: 0 658 1176
Size: 658 518
AleI CACnn'nnGTG
Cuts at: 0 913 1176
Size: 913 263
AlwNI CAG_nnn'CTG
Cuts at: 0 932 1176
Size: 932 244
ApoI r'AATT_y
Cuts at: 0 467 481 495 520 1176
Size: 467 14 14 25 656
Fragments arranged by size:
656 467 25 14 14
AvaI C'yCGr_G
Cuts at: 0 28 428 1033 1055 1176
Size: 28 400 605 22 121
Fragments arranged by size:
605 400 121 28 22
BanII G_rGCy'C
Cuts at: 0 29 983 1135 1176
Size: 29 954 152 41
Fragments arranged by size:
954 152 41 29
BbvCI CC'TCA_GC
Cuts at: 0 766 1176
Size: 766 410
BciVI GTATCCnnnnn_n'
Cuts at: 0 803 1176
Size: 803 373
BglI GCCn_nnn'nGGC
Cuts at: 0 1117 1176
Size: 1117 59
BlpI GC'TnA_GC
Cuts at: 0 335 1176
Size: 335 841
Bme1580I G_kGCm'C
Cuts at: 0 920 1175 1176
Size: 920 255 1
Fragments arranged by size:
920 255 1
BmgBI CAC'GTC
Cuts at: 0 375 1176
Size: 375 801
BmtI G_CTAG'C
Cuts at: 0 164 1176
Size: 164 1012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1176
Size: 842 334
Bpu10I CC'TnA_GC
Cuts at: 0 766 1176
Size: 766 410
BsaAI yAC'GTr
Cuts at: 0 659 1176
Size: 659 517
BsaHI Gr'CG_yC
Cuts at: 0 1079 1176
Size: 1079 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 815 845 1176
Size: 815 30 331
Fragments arranged by size:
815 331 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 412 751 1176
Size: 370 42 339 425
Fragments arranged by size:
425 370 339 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1144 1176
Size: 473 671 32
Fragments arranged by size:
671 473 32
BsiEI CG_ry'CG
Cuts at: 0 1139 1176
Size: 1139 37
Bsp1286I G_dGCh'C
Cuts at: 0 29 920 983 1135 1175 1176
Size: 29 891 63 152 40 1
Fragments arranged by size:
891 152 63 40 29 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 1176
Size: 26 1150
BsrBI CCG'CTC
Cuts at: 0 339 801 1176
Size: 339 462 375
Fragments arranged by size:
462 375 339
BstYI r'GATC_y
Cuts at: 0 1046 1176
Size: 1046 130
BtsI GCAGTG_nn'
Cuts at: 0 171 270 650 1176
Size: 171 99 380 526
Fragments arranged by size:
526 380 171 99
EaeI y'GGCC_r
Cuts at: 0 101 1109 1136 1176
Size: 101 1008 27 40
Fragments arranged by size:
1008 101 40 27
EagI C'GGCC_G
Cuts at: 0 1136 1176
Size: 1136 40
EarI CTCTTCn'nnn_
Cuts at: 0 13 245 263 306 556 593 791 988
Size: 13 232 18 43 250 37 198 197
Cuts at: 988 1176
Size: 188
Fragments arranged by size:
250 232 198 197 188 43 37 18
13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 340 842 1176
Size: 236 104 502 334
Fragments arranged by size:
502 334 236 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1101 1176
Size: 1101 75
EcoO109I rG'GnC_Cy
Cuts at: 0 15 73 130 407 1176
Size: 15 58 57 277 769
Fragments arranged by size:
769 277 58 57 15
EcoRI G'AATT_C
Cuts at: 0 467 1176
Size: 467 709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 622 1176
Size: 622 554
HaeII r_GCGC'y
Cuts at: 0 36 900 1176
Size: 36 864 276
Fragments arranged by size:
864 276 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 106 138 300 332 524 556
Size: 65 32 9 32 162 32 192 32
Cuts at: 556 815 847 1176
Size: 259 32 329
Fragments arranged by size:
329 259 192 162 65 32 32 32
32 32 9
HindIII A'AGCT_T
Cuts at: 0 614 1176
Size: 614 562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1176
Size: 808 368
MscI TGG'CCA
Cuts at: 0 1111 1176
Size: 1111 65
MslI CAynn'nnrTG
Cuts at: 0 593 913 1153 1176
Size: 593 320 240 23
Fragments arranged by size:
593 320 240 23
MspA1I CmG'CkG
Cuts at: 0 939 1128 1176
Size: 939 189 48
Fragments arranged by size:
939 189 48
NheI G'CTAG_C
Cuts at: 0 160 1176
Size: 160 1016
NspI r_CATG'y
Cuts at: 0 754 1176
Size: 754 422
PmlI CAC'GTG
Cuts at: 0 659 1176
Size: 659 517
PpuMI rG'GwC_Cy
Cuts at: 0 15 73 130 1176
Size: 15 58 57 1046
Fragments arranged by size:
1046 58 57 15
PsiI TTA'TAA
Cuts at: 0 731 1176
Size: 731 445
PvuII CAG'CTG
Cuts at: 0 939 1176
Size: 939 237
SapI GCTCTTCn'nnn_
Cuts at: 0 263 791 988 1176
Size: 263 528 197 188
Fragments arranged by size:
528 263 197 188
ScaI AGT'ACT
Cuts at: 0 111 1176
Size: 111 1065
SfcI C'TryA_G
Cuts at: 0 671 1176
Size: 671 505
SmaI CCC'GGG
Cuts at: 0 1057 1176
Size: 1057 119
SmlI C'TyrA_G
Cuts at: 0 759 1033 1176
Size: 759 274 143
Fragments arranged by size:
759 274 143
TatI w'GTAC_w
Cuts at: 0 109 890 1176
Size: 109 781 286
Fragments arranged by size:
781 286 109
Tth111I GACn'n_nGTC
Cuts at: 0 1144 1176
Size: 1144 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 663 1176
Size: 663 513
XhoI C'TCGA_G
Cuts at: 0 1033 1176
Size: 1033 143
XmaI C'CCGG_G
Cuts at: 0 1055 1176
Size: 1055 121
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AleI AlwNI ApoI
AvaI BanII BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmtI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BsgI
BsiEI Bsp1286I BspMI BsrBI BstYI BtsI EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NheI NspI PmlI
PpuMI PsiI PvuII SapI ScaI SfcI SmaI SmlI
TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BcgI BclI BfrBI BglII BmrI
BplI BpuEI BsaI BsaBI BsaWI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI XbaI XmnI ZraI