Restriction map for
pFN21AE1338
(Circular) (Six-base) MAPSORT of: pFN21AE1338.seq Check: 5013 from: 1 to: 5881
pFN21AE1338 5881 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2679 3224 1937
Size: 742 545 4594
Fragments arranged by size:
4594 742 545
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 2345 3944 5328 5596 1632
Size: 609 104 1599 1384 268 1917
Fragments arranged by size:
1917 1599 1384 609 268 104
AfeI AGC'GCT
Cuts at: 2039 2903 2039
Size: 864 5017
AflII C'TTAA_G
Cuts at: 820 1017 2764 820
Size: 197 1747 3937
Fragments arranged by size:
3937 1747 197
AflIII A'CryG_T
Cuts at: 1203 2663 4780 5757 1203
Size: 1460 2117 977 1327
Fragments arranged by size:
2117 1460 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 2918 1166
Size: 1752 4129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2937 3624 5196 2937
Size: 687 1572 3622
Fragments arranged by size:
3622 1572 687
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2472 2486 2500 2525 3190 3416 3568 5677
Size: 979 14 14 25 665 226 152 2109
Cuts at: 5677 1493
Size: 1697
Fragments arranged by size:
2109 1697 979 665 226 152 25 14
14
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2433 3038 3060 3206 1870
Size: 73 90 400 605 22 146 4545
Fragments arranged by size:
4545 605 400 146 90 73 22
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3202 4657 618
Size: 325 217 627 1415 1455 1842
Fragments arranged by size:
1842 1455 1415 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2988 3140 3200 1391
Size: 643 954 152 60 4072
Fragments arranged by size:
4072 954 643 152 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BbvCI CC'TCA_GC
Cuts at: 2771 2771
Size: 5881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2808 3773 4983 2808
Size: 965 1210 3706
Fragments arranged by size:
3706 1210 965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3122 4492 136
Size: 107 122 71 631 605 1450 1370 1525
Fragments arranged by size:
1525 1450 1370 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 2340 3298 2340
Size: 958 4923
Bme1580I G_kGCm'C
Cuts at: 1790 2925 3180 3942 5098 1790
Size: 1135 255 762 1156 2573
Fragments arranged by size:
2573 1156 1135 762 255
BmgBI CAC'GTC
Cuts at: 1318 2380 1318
Size: 1062 4819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2169 1056
Size: 1113 4768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2847 4541 1239
Size: 1608 1694 2579
Fragments arranged by size:
2579 1694 1608
Bpu10I CC'TnA_GC
Cuts at: 2771 2771
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2664 3672 493
Size: 711 1460 1008 2702
Fragments arranged by size:
2702 1460 1008 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3084 3688 4070 4705
Size: 53 83 186 936 1551 604 382 635
Cuts at: 4705 275
Size: 1451
Fragments arranged by size:
1551 1451 936 635 604 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2820 2850 1178
Size: 30 165 30 1417 30 4209
Fragments arranged by size:
4209 1417 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2417 2756 1186
Size: 1189 42 339 4311
Fragments arranged by size:
4311 1189 339 42
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 3149 1163
Size: 573 742 671 3895
Fragments arranged by size:
3895 742 671 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3144 3331 4092 5120 1540
Size: 275 186 1143 187 761 1028 2301
Fragments arranged by size:
2301 1143 1028 761 275 187 186
BsiHKAI G_wGCw'C
Cuts at: 3942 4027 5098 3942
Size: 85 1071 4725
Fragments arranged by size:
4725 1071 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2925 2988 3140 3180 3200 3942
Size: 399 244 891 63 152 40 20 742
Cuts at: 3942 4027 5098 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 891 742 399 244 152 85
63 40 20
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 3236 844
Size: 1187 1205 3489
Fragments arranged by size:
3489 1205 1187
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 2806 3768 1394
Size: 429 521 462 962 3507
Fragments arranged by size:
3507 962 521 462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 3051 3211 3578 3963 3980 5432 5518 5530
Size: 1079 160 367 385 17 1452 86 12
Cuts at: 5530 5876 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1079 385 367 346 160 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4390
Fragments arranged by size:
4390 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 2176 2275 2655 3397 4191 4211 2176
Size: 99 380 742 794 20 3846
Fragments arranged by size:
3846 794 742 380 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 3114 3141
Size: 54 1425 50 275 18 276 1008 27
Cuts at: 3141 3328 4217 5846 8
Size: 187 889 1629 43
Fragments arranged by size:
1629 1425 1008 889 276 275 187 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3141 3328 1537
Size: 275 1329 187 4090
Fragments arranged by size:
4090 1329 275 187
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2250 2268 2311 2561 2598 2796 2993
Size: 665 232 18 43 250 37 198 197
Cuts at: 2993 3811 1353
Size: 818 3423
Fragments arranged by size:
3423 818 665 250 232 198 197 43
37 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2345 2847 3944 4541 5328 5596
Size: 393 609 104 502 1097 597 787 268
Cuts at: 5596 1239
Size: 1524
Fragments arranged by size:
1524 1097 787 609 597 502 393 268
104
EcoNI CCTnn'n_nnAGG
Cuts at: 3106 3106
Size: 5881
EcoO109I rG'GnC_Cy
Cuts at: 2020 2078 2135 2412 2020
Size: 58 57 277 5489
Fragments arranged by size:
5489 277 58 57
EcoRI G'AATT_C
Cuts at: 2472 3190 3568 2472
Size: 718 378 4785
Fragments arranged by size:
4785 718 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2627 2627
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2041 2905 5028 1678
Size: 363 864 2123 2531
Fragments arranged by size:
2531 2123 864 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2111 2143 2305
Size: 32 135 32 498 32 9 32 162
Cuts at: 2305 2337 2529 2561 2820 2852 4522 4554 4596
Size: 32 192 32 259 32 1670 32 42
Cuts at: 4596 4628 1373
Size: 32 2626
Fragments arranged by size:
2626 1670 498 259 192 162 135 42
32 32 32 32 32 32 32 32
32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2619 748
Size: 1871 4010
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2813 4995 5179 1305
Size: 1508 2182 184 2007
Fragments arranged by size:
2182 2007 1508 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3116 10
Size: 54 1425 343 1284 2775
Fragments arranged by size:
2775 1425 1284 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2598 2918 3158 3839 4198 4357 518
Size: 648 1432 320 240 681 359 159 2042
Fragments arranged by size:
2042 1432 681 648 359 320 240 159
MspA1I CmG'CkG
Cuts at: 1606 2944 3133 3297 3974 5122 5367 1606
Size: 1338 189 164 677 1148 245 2120
Fragments arranged by size:
2120 1338 1148 677 245 189 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4390
Fragments arranged by size:
4390 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 2165 1052
Size: 1113 4768
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2759 3239 4784 1132
Size: 97 1530 480 1545 2229
Fragments arranged by size:
2229 1545 1530 480 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PmlI CAC'GTG
Cuts at: 2664 2664
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2078 2135 2020
Size: 58 57 5766
Fragments arranged by size:
5766 58 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 2736 3451 2736
Size: 715 5166
PstI C_TGCA'G
Cuts at: 830 3233 830
Size: 2403 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2944 2944
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SapI GCTCTTCn'nnn_
Cuts at: 2268 2796 2993 2268
Size: 528 197 5156
Fragments arranged by size:
5156 528 197
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 2116 4129 5841 2116
Size: 2013 1712 2156
Fragments arranged by size:
2156 2013 1712
SfcI C'TryA_G
Cuts at: 826 1045 2676 3229 4364 5045 5236 826
Size: 219 1631 553 1135 681 191 1471
Fragments arranged by size:
1631 1471 1135 681 553 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3062 3208 1872
Size: 1190 146 4545
Fragments arranged by size:
4545 1190 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2764 3038 3985 4886 5148 820
Size: 197 926 821 274 947 901 262 1553
Fragments arranged by size:
1553 947 926 901 821 274 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 3320 513
Size: 552 939 1316 3074
Fragments arranged by size:
3074 1316 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3908 3925 4078 4263 4708 325
Size: 1255 2328 17 153 185 445 1498
Fragments arranged by size:
2328 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2114 2895 4127
Size: 274 80 33 51 1443 137 781 1232
Cuts at: 4127 5839 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1232 781 274 138 137 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 3149 1316
Size: 1833 4048
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2668 2668
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 3038 1943
Size: 1095 4786
XmaI C'CCGG_G
Cuts at: 1870 3060 3206 1870
Size: 1190 146 4545
Fragments arranged by size:
4545 1190 146
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI