MAGEF1 ORF clone pFN21AE1132

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Halotag ORF Clone pFN21AE1132

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Clone info.

Species

human

Clone Name

pFN21AE1132 Sequence (with vector) Restriction Map

Product ID

FHC10810

ORF Sequence

DNA sequence (924 bp)
Protein sequence (308 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590456]

Gene

KIEE1132

Symbol

MAGEF1

Alias : MAGE-F1

Description

melanoma antigen family F1

Original Clone ID

cs03005

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 924 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 308 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3269.1 1.2e-122 99.4 MAGEF1 CCDS10023.1 1e-56 51.0 NSMCE3 CCDS14433.1 2.7e-41 52.1 MAGEE1 CCDS14362.1 4.8e-40 41.6 MAGED2 CCDS14216.1 2.3e-36 40.6 MAGEB18 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002190 83 255 PF01454 MAGE protein ProfileScan IPR002190 76 277 PS50838 MAGE protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_071432 307 609267 99.4 100.0 NP_619649 304 608243 51.0 95.1 NP_957516 606 300470,300971 41.6 96.1 NP_055414 606 300470,300971 41.6 96.1 NP_803182 606 300470,300971 41.6 96.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1132 Download

>KIEE1132  924 bp
ATGTTGCAGACACCAGAGAGCAGGGGGCTCCCGGTCCCGCAGGCCGAGGGGGAGAAGGAT
GGCGGCCATGATGGTGAGACCCGGGCCCCGACCGCCTCGCAGGAGCGCCCCAAGGAGGAG
CTTGGCGCCGGGAGGGAGGAGGGGGCTGCGGAGCCCGCCCTCACCCGGAAAGGCGCGAGG
GCCTTGGCGGCCAAAGCCTTGGCAAGGCGCAGGGCCTACCGCCGGCTGAATCGGACGGTG
GCGGAGTTGGTGCAGTTCCTCCTGGTGAAAGACAAGAAGAAGAGTCCCATCACACGCTCG
GAGATGGTGAAATACGTTATTGGAGACTTGAAGATTCTGTTCCCGGACATCATCGCAAGG
GCCGCAGAGCATCTGCGGTATGTCTTTGGTTTTGAGCTGAAACAGTTTGACCGCAAGCAC
CACACTTACATCCTGATCAACAAACTAAAACCTCTGGAGGAGGAGGAGGAGGAGGAGGAT
CTGGGAGGAGATGGCCCCAGATTGGGTCTGTTAATGATGATCCTGGGCCTTATCTATATG
AGAGGTAATAGCGCCAGGGAGGCCCAGGTCTGGGAGATGCTGCGTCGGTTGGGGGTGCAA
CCCTCAAAGTATCATTTCCTCTTTGGGTATCCGAAGAGGCTTATTATGGAAGATTTTGTG
CAGCAGCGATATCTCAGTTACAGGCGGGTGCCTCACACCAATCCACCAGCATATGAATTC
TCTTGGGGTCCCCGAAGCAACCTGGAAATCAGCAAGATGGAAGTCCTGGGGTTCGTGGCC
AAACTGCATAAGAAGGAACCGCAGCACTGGCCAGTGCAGTACCGTGAGGCCCTAGCAGAC
GAGGCCGACAGGGCCAGAGCCAAGGCCAGAGCTGAAGCCAGTATGAGGGCCAGGGCCAGT
GCTAGGGCCGGCATCCACCTCTGG

ORF Protein

Cloned ORF protein sequence for pFN21AE1132 Download

>KIEE1132  308 aa
MLQTPESRGLPVPQAEGEKDGGHDGETRAPTASQERPKEELGAGREEGAAEPALTRKGAR
ALAAKALARRRAYRRLNRTVAELVQFLLVKDKKKSPITRSEMVKYVIGDLKILFPDIIAR
AAEHLRYVFGFELKQFDRKHHTYILINKLKPLEEEEEEEDLGGDGPRLGLLMMILGLIYM
RGNSAREAQVWEMLRRLGVQPSKYHFLFGYPKRLIMEDFVQQRYLSYRRVPHTNPPAYEF
SWGPRSNLEISKMEVLGFVAKLHKKEPQHWPVQYREALADEADRARAKARAEASMRARAS
ARAGIHLW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1132 Download

>pFN21AE1132  5629 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTGCAGACACCAGAGAGCAGGGGGCTCCCGGT
CCCGCAGGCCGAGGGGGAGAAGGATGGCGGCCATGATGGTGAGACCCGGGCCCCGACCGC
CTCGCAGGAGCGCCCCAAGGAGGAGCTTGGCGCCGGGAGGGAGGAGGGGGCTGCGGAGCC
CGCCCTCACCCGGAAAGGCGCGAGGGCCTTGGCGGCCAAAGCCTTGGCAAGGCGCAGGGC
CTACCGCCGGCTGAATCGGACGGTGGCGGAGTTGGTGCAGTTCCTCCTGGTGAAAGACAA
GAAGAAGAGTCCCATCACACGCTCGGAGATGGTGAAATACGTTATTGGAGACTTGAAGAT
TCTGTTCCCGGACATCATCGCAAGGGCCGCAGAGCATCTGCGGTATGTCTTTGGTTTTGA
GCTGAAACAGTTTGACCGCAAGCACCACACTTACATCCTGATCAACAAACTAAAACCTCT
GGAGGAGGAGGAGGAGGAGGAGGATCTGGGAGGAGATGGCCCCAGATTGGGTCTGTTAAT
GATGATCCTGGGCCTTATCTATATGAGAGGTAATAGCGCCAGGGAGGCCCAGGTCTGGGA
GATGCTGCGTCGGTTGGGGGTGCAACCCTCAAAGTATCATTTCCTCTTTGGGTATCCGAA
GAGGCTTATTATGGAAGATTTTGTGCAGCAGCGATATCTCAGTTACAGGCGGGTGCCTCA
CACCAATCCACCAGCATATGAATTCTCTTGGGGTCCCCGAAGCAACCTGGAAATCAGCAA
GATGGAAGTCCTGGGGTTCGTGGCCAAACTGCATAAGAAGGAACCGCAGCACTGGCCAGT
GCAGTACCGTGAGGCCCTAGCAGACGAGGCCGACAGGGCCAGAGCCAAGGCCAGAGCTGA
AGCCAGTATGAGGGCCAGGGCCAGTGCTAGGGCCGGCATCCACCTCTGGGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT