(Circular) (Six-base) MAPSORT of: pFN21AE1132.seq Check: 7115 from: 1 to: 5629 pFN21AE1132 5629 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2898 3692 5076 5344 1632 Size: 1266 794 1384 268 1917 Fragments arranged by size: 1917 1384 1266 794 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaI G_GGCC'C Cuts at: 2092 2092 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2720 2938 3164 3316 5425 1493 Size: 1227 218 226 152 2109 1697 Fragments arranged by size: 2109 1697 1227 226 218 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2954 1870 Size: 73 142 869 4545 Fragments arranged by size: 4545 869 142 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2692 2950 4405 618 Size: 325 217 627 342 563 258 1455 1842 Fragments arranged by size: 1842 1455 627 563 342 325 258 217 BanII G_rGCy'C Cuts at: 1391 2034 2092 2160 2948 1391 Size: 643 58 68 788 4072 Fragments arranged by size: 4072 788 643 68 58 BbeI G_GCGC'C Cuts at: 2133 2133 Size: 5629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2643 3521 4731 2643 Size: 878 1210 3541 Fragments arranged by size: 3541 1210 878 BclI T'GATC_A Cuts at: 1548 2439 1548 Size: 891 4738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 2564 2855 Size: 107 122 71 631 605 520 372 291 Cuts at: 2855 4240 136 Size: 1385 1525 Fragments arranged by size: 1525 1385 631 605 520 372 291 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2092 3690 4846 1790 Size: 302 1598 1156 2573 Fragments arranged by size: 2573 1598 1156 302 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 4289 1239 Size: 1241 1809 2579 Fragments arranged by size: 2579 1809 1241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 4292 882 Size: 617 576 2217 2219 Fragments arranged by size: 2219 2217 617 576 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 3436 3818 4453 Size: 53 83 186 936 597 1306 382 635 Cuts at: 4453 275 Size: 1451 Fragments arranged by size: 1451 1306 936 635 597 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2156 2477 2480 2483 2486 2489 2492 Size: 949 21 321 3 3 3 3 3 Cuts at: 2492 2505 1186 Size: 13 4310 Fragments arranged by size: 4310 949 321 21 13 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 4832 5328 4832 Size: 496 5133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 2684 2840 1163 Size: 573 540 408 156 3952 Fragments arranged by size: 3952 573 540 408 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2097 3079 3840 4868 1540 Size: 275 186 96 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 96 BsiHKAI G_wGCw'C Cuts at: 3690 3775 4846 3690 Size: 85 1071 4473 Fragments arranged by size: 4473 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2092 2160 2948 3690 3775 4846 Size: 399 244 58 68 788 742 85 1071 Cuts at: 4846 1391 Size: 2174 Fragments arranged by size: 2174 1071 788 742 399 244 85 68 58 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 2912 4273 4460 1700 Size: 110 416 686 1361 187 2869 Fragments arranged by size: 2869 1361 686 416 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2203 2203 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2482 2959 3326 3711 3728 5180 5266 5278 Size: 510 477 367 385 17 1452 86 12 Cuts at: 5278 5624 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 510 477 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5077 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2341 487 Size: 683 56 1115 3775 Fragments arranged by size: 3775 1115 683 56 BtsI GCAGTG_nn' Cuts at: 3145 3939 3959 3145 Size: 794 20 4815 Fragments arranged by size: 4815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2193 2781 Size: 54 1425 50 275 18 238 125 588 Cuts at: 2781 2813 3076 3965 5594 8 Size: 32 263 889 1629 43 Fragments arranged by size: 1629 1425 889 588 275 263 238 125 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 2633 3559 1353 Size: 926 354 926 3423 Fragments arranged by size: 3423 926 926 354 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2261 4216 4590 4736 387 Size: 1064 138 672 1955 374 146 1280 Fragments arranged by size: 1955 1280 1064 672 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 2898 3692 4289 5076 5344 1239 Size: 393 848 418 794 597 787 268 1524 Fragments arranged by size: 1524 848 794 787 597 418 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2206 2206 Size: 5629 EcoO109I rG'GnC_Cy Cuts at: 2089 2184 2217 2732 2833 2089 Size: 95 33 515 101 4885 Fragments arranged by size: 4885 515 101 95 33 EcoRI G'AATT_C Cuts at: 2720 2938 3316 2720 Size: 218 378 5033 Fragments arranged by size: 5033 378 218 EcoRV GAT'ATC Cuts at: 2675 2675 Size: 5629 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 2113 2133 2559 4776 1678 Size: 435 20 426 2217 2531 Fragments arranged by size: 2531 2217 435 426 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2572 2604 4270 4302 4344 Size: 32 135 32 1000 32 1666 32 42 Cuts at: 4344 4376 1373 Size: 32 2626 Fragments arranged by size: 2626 1666 1000 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KasI G'GCGC_C Cuts at: 2129 2129 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2783 2815 10 Size: 54 1425 343 951 32 2824 Fragments arranged by size: 2824 1425 951 343 54 32 MslI CAynn'nnrTG Cuts at: 518 1166 2076 3587 3946 4105 518 Size: 648 910 1511 359 159 2042 Fragments arranged by size: 2042 1511 910 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3045 3722 4870 5115 1606 Size: 1439 677 1148 245 2120 Fragments arranged by size: 2120 1439 1148 677 245 NaeI GCC'GGC Cuts at: 2228 2914 2228 Size: 686 4943 NarI GG'CG_CC Cuts at: 2130 2130 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5077 NdeI CA'TA_TG Cuts at: 387 2716 387 Size: 2329 3300 NgoMIV G'CCGG_C Cuts at: 2226 2912 2226 Size: 686 4943 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2987 4532 1132 Size: 97 1758 1545 2229 Fragments arranged by size: 2229 1758 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PfoI T'CCnGG_A Cuts at: 2346 2346 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2732 2732 Size: 5629 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2088 2088 Size: 5629 PstI C_TGCA'G Cuts at: 830 2981 830 Size: 2151 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SanDI GG'GwC_CC Cuts at: 2732 2732 Size: 5629 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2977 4112 4793 4984 826 Size: 219 1932 1135 681 191 1471 Fragments arranged by size: 1932 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2192 2855 2192 Size: 663 4966 SfoI GGC'GCC Cuts at: 2131 2131 Size: 5629 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2087 2956 1872 Size: 215 869 4545 Fragments arranged by size: 4545 869 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 2115 2187 2202 2865 3068 513 Size: 552 1050 72 15 663 203 3074 Fragments arranged by size: 3074 1050 663 552 203 72 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 3673 3826 4011 4456 325 Size: 1255 2076 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2085 2954 1870 Size: 215 869 4545 Fragments arranged by size: 4545 869 215 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SrfI SwaI XcmI