Restriction map for
pFN21AE1132
(Linear) (Six-base) MAPSORT of: KIEE1132.seq Check: 1736 from: 1 to: 924
KIEE1132.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 924
Size: 893 31
ApaI G_GGCC'C
Cuts at: 0 87 924
Size: 87 837
ApoI r'AATT_y
Cuts at: 0 715 924
Size: 715 209
AvaI C'yCGr_G
Cuts at: 0 80 924
Size: 80 844
BanI G'GyrC_C
Cuts at: 0 124 687 924
Size: 124 563 237
Fragments arranged by size:
563 237 124
BanII G_rGCy'C
Cuts at: 0 29 87 155 924
Size: 29 58 68 769
Fragments arranged by size:
769 68 58 29
BbeI G_GCGC'C
Cuts at: 0 128 924
Size: 128 796
BciVI GTATCCnnnnn_n'
Cuts at: 0 638 924
Size: 638 286
BclI T'GATC_A
Cuts at: 0 434 924
Size: 434 490
BglI GCCn_nnn'nGGC
Cuts at: 0 187 559 850 924
Size: 187 372 291 74
Fragments arranged by size:
372 291 187 74
Bme1580I G_kGCm'C
Cuts at: 0 87 924
Size: 87 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 924
Size: 475 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 924
Size: 70 854
BsaHI Gr'CG_yC
Cuts at: 0 125 924
Size: 125 799
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 151 472 475 478 481 484 487
Size: 130 21 321 3 3 3 3 3
Cuts at: 487 500 924
Size: 13 424
Fragments arranged by size:
424 321 130 21 13 3 3 3
3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 679 835 924
Size: 271 408 156 89
Fragments arranged by size:
408 271 156 89
BsiEI CG_ry'CG
Cuts at: 0 92 924
Size: 92 832
Bsp1286I G_dGCh'C
Cuts at: 0 29 87 155 924
Size: 29 58 68 769
Fragments arranged by size:
769 68 58 29
BsrFI r'CCGG_y
Cuts at: 0 221 907 924
Size: 221 686 17
Fragments arranged by size:
686 221 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 198 924
Size: 198 726
BstYI r'GATC_y
Cuts at: 0 477 924
Size: 477 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 336 924
Size: 336 588
EaeI y'GGCC_r
Cuts at: 0 63 188 776 808 924
Size: 63 125 588 32 116
Fragments arranged by size:
588 125 116 63 32
EarI CTCTTCn'nnn_
Cuts at: 0 274 628 924
Size: 274 354 296
Fragments arranged by size:
354 296 274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 924
Size: 256 668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 893 924
Size: 475 418 31
Fragments arranged by size:
475 418 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 201 924
Size: 201 723
EcoO109I rG'GnC_Cy
Cuts at: 0 84 179 212 727 828 924
Size: 84 95 33 515 101 96
Fragments arranged by size:
515 101 96 95 84 33
EcoRI G'AATT_C
Cuts at: 0 715 924
Size: 715 209
EcoRV GAT'ATC
Cuts at: 0 670 924
Size: 670 254
HaeII r_GCGC'y
Cuts at: 0 108 128 554 924
Size: 108 20 426 370
Fragments arranged by size:
426 370 108 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 567 599 924
Size: 567 32 325
Fragments arranged by size:
567 325 32
KasI G'GCGC_C
Cuts at: 0 124 924
Size: 124 800
MscI TGG'CCA
Cuts at: 0 778 810 924
Size: 778 32 114
Fragments arranged by size:
778 114 32
MslI CAynn'nnrTG
Cuts at: 0 71 924
Size: 71 853
NaeI GCC'GGC
Cuts at: 0 223 909 924
Size: 223 686 15
Fragments arranged by size:
686 223 15
NarI GG'CG_CC
Cuts at: 0 125 924
Size: 125 799
NdeI CA'TA_TG
Cuts at: 0 711 924
Size: 711 213
NgoMIV G'CCGG_C
Cuts at: 0 221 907 924
Size: 221 686 17
Fragments arranged by size:
686 221 17
PfoI T'CCnGG_A
Cuts at: 0 341 924
Size: 341 583
PpuMI rG'GwC_Cy
Cuts at: 0 727 924
Size: 727 197
PspOMI G'GGCC_C
Cuts at: 0 83 924
Size: 83 841
SanDI GG'GwC_CC
Cuts at: 0 727 924
Size: 727 197
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 187 850 924
Size: 187 663 74
Fragments arranged by size:
663 187 74
SfoI GGC'GCC
Cuts at: 0 126 924
Size: 126 798
SmaI CCC'GGG
Cuts at: 0 82 924
Size: 82 842
StyI C'CwwG_G
Cuts at: 0 110 182 197 860 924
Size: 110 72 15 663 64
Fragments arranged by size:
663 110 72 64 15
XmaI C'CCGG_G
Cuts at: 0 80 924
Size: 80 844
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanI BanII BbeI BciVI
BclI BglI Bme1580I BpmI BsaI BsaHI BseRI BsgI
BsiEI Bsp1286I BsrFI BstXI BstYI BtgZI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
KasI MscI MslI NaeI NarI NdeI NgoMIV PfoI
PpuMI PspOMI SanDI SfiI SfoI SmaI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MspA1I NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI