Restriction map for
pFN21AE1132
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 174 CGCG CG^CG
AcsI 715 GAATTC [GA]^AATT[CT]
AcyI 124 GGCGCC G[GA]^CG[CT]C
AfaI 819 GTAC GT^AC
ApaI 83 GGGCCC GGGCC^C
ApoI 715 GAATTC [GA]^AATT[CT]
AvaI 80 CCCGGG C^[CT]CG[GA]G
BbeI 124 GGCGCC GGCGC^C
BciVI 627 GTATCC GTATCC(6/5)
BclI 434 TGATCA T^GATCA
BpmI 454 CTGGAG CTGGAG(16/14)
BsaHI 124 GGCGCC G[GA]^CG[CT]C
BsaI 76 GAGACC GGTCTC(1/5)
BsiEI 89 CGACCG CG[GA][CT]^CG
BsoBI 80 CCCGGG C^[CT]CG[GA]G
BstUI 174 CGCG CG^CG
BstXI 191 CCAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 477 GGATCT [GA]^GATC[CT]
DdeI 673 CTCAG C^T[ACGT]AG
EcoNI 197 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 715 GAATTC G^AATTC
EcoRV 668 GATATC GAT^ATC
FbaI 434 TGATCA T^GATCA
HgaI 582 GCGTC GACGC(5/10)
Hin1I 124 GGCGCC G[GA]^CG[CT]C
KasI 124 GGCGCC G^GCGCC
MaeII 314 ACGT A^CGT
MaeIII 677 GTTAC ^GT[ACGT]AC
MflI 477 GGATCT [GA]^GATC[CT]
MseI 511 TTAA T^TAA
MslI 67 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 174 CGCG CG^CG
NarI 124 GGCGCC GG^CGCC
NdeI 710 CATATG CA^TATG
NlaIII 67 CATG CATG^
PleI 282 GAGTC GAGTC(4/5)
PpuMI 726 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 819 GTAC GT^AC
SfoI 124 GGCGCC GGC^GCC
SmaI 80 CCCGGG CCC^GGG
Tru9I 511 TTAA T^TAA
Tsp509I 716 AATT ^AATT
XhoII 477 GGATCT [GA]^GATC[CT]
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 519 GATCC GGATC(4/5)
477 GGATC
AvaII 33 GGTCC G^G[AT]CC
727 GGTCC
BalI 776 TGGCCA TGG^CCA
808 TGGCCA
BanI 124 GGCGCC G^G[CT][GA]CC
687 GGTGCC
BfaI 832 CTAG C^TAG
902 CTAG
BsmAI 76 GAGAC GTCTC(1/5)
323 GAGAC
BsrFI 221 GCCGGC [GA]^CCGG[CT]
907 GCCGGC
Cfr10I 221 GCCGGC [GA]^CCGG[CT]
907 GCCGGC
EarI 279 GAAGAG CTCTTC(1/4)
633 GAAGAG
Ksp632I 279 GAAGAG CTCTTC(1/4)
633 GAAGAG
MaeI 832 CTAG C^TAG
902 CTAG
MluNI 776 TGGCCA TGG^CCA
808 TGGCCA
MscI 776 TGGCCA TGG^CCA
808 TGGCCA
NaeI 221 GCCGGC GCC^GGC
907 GCCGGC
NgoMIV 221 GCCGGC G^CCGGC
907 GCCGGC
SfiI 180 GGCCTTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
843 GGCCGACAGGGCC
TfiI 228 GAATC G^A[AT]TC
333 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BglI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TseI TspRI