SBK1 ORF clone pFN21AE0744

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0744

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Clone info.

Species

human

Clone Name

pFN21AE0744 Sequence (with vector) Restriction Map

Product ID

FHC28376

ORF Sequence

DNA sequence (1272 bp)
Protein sequence (424 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE0744

Symbol

SBK1

Alias : SBK

Description

SH3 domain binding kinase 1

Original Clone ID

cs02244

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1272 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 424 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32416.1 5.3e-76 100.0 SBK1 CCDS42631.1 1.3e-15 42.9 SBK2 CCDS74457.1 6.2e-14 39.9 SBK3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 53 310 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 53 318 SM00220 Serine/threonine-protein kinase domain IPR020635 53 313 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 53 318 PS50011 Protein kinase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 230 VDTGVDVWAFGVLIFCVLTGNFP 252 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0744 Download

>KIEE0744  1272 bp
ATGAGCGTGGGCTGCCCAGAGCCTGAGCCGCCCCGCTCCCTGACCTGCTGTGGGCCGGGG
ACTGCCCCTGGGCCTGGTGCCGGTGTGCCCCTTCTCACTGAAGACATGCAGGCCCTGACT
CTCCGCACACTGGCCGCCAGCGACGTCACCAAGCACTACGAACTAGTCCGGGAGCTGGGC
AAAGGCACCTATGGGAAGGTTGACCTGGTGGTCTACAAGGGCACAGGCACAAAAATGGCA
CTGAAGTTTGTGAACAAGAGCAAAACCAAGCTGAAGAACTTCCTACGGGAGGTGAGCATC
ACCAACAGCCTCTCCTCCAGCCCCTTCATCATCAAGGTCTTTGACGTGGTCTTTGAGACA
GAGGACTGCTACGTCTTTGCCCAGGAGTACGCACCTGCTGGGGACCTGTTTGACATCATC
CCTCCCCAGGTGGGGCTCCCTGAGGACACGGTGAAGCGCTGTGTGCAGCAGCTGGGCCTG
GCGCTGGACTTCATGCACGGGCGGCAGCTGGTGCACCGCGACATCAAGCCCGAGAACGTG
CTGCTGTTCGACCGCGAGTGCCGCCGCGTAAAGCTGGCCGACTTCGGCATGACGCGCCGC
GTGGGCTGCCGCGTCAAGCGCGTGAGCGGCACCATCCCTTACACGGCGCCTGAGGTGTGC
CAGGCGGGCCGCGCCGACGGGCTGGCGGTGGACACGGGCGTGGACGTGTGGGCCTTCGGC
GTGCTCATCTTCTGCGTGCTCACCGGCAACTTCCCGTGGGAGGCGGCGTCGGGCGCCGAC
GCCTTCTTCGAGGAGTTCGTGCGCTGGCAGCGGGGCCGCCTGCCGGGGCTGCCTTCGCAG
TGGCGCCGCTTCACCGAGCCCGCGCTGCGCATGTTCCAGCGCTTACTGGCCCTGGAGCCC
GAGCGCCGCGGCCCAGCCAAGGAGGTGTTCCGCTTCCTCAAGCACGAGCTCACGTCCGAG
CTGCGCCGCCGGCCCTCGCACCGCGCGCGCAAGCCCCCCGGGGACCGCCCGCCCGCCGCC
GGGCCACTGCGCCTCGAGGCGCCTGGGCCGCTCAAGCGGACGGTGCTGACCGAGAGCGGC
AGCGGCTCCCGGCCCGCGCCCCCCGCCGTCGGGTCGGTGCCCTTGCCCGTGCCGGTGCCG
GTGCCAGTGCCCGTGCCGGTGCCTGTGCCCGAGCCCGGCCTAGCTCCCCAGGGGCCCCCC
GGCCGGACCGACGGCCGCGCGGACAAGAGCAAAGGGCAGGTGGTGCTGGCCACGGCCATC
GAGATCTGCGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE0744 Download

>KIEE0744  424 aa
MSVGCPEPEPPRSLTCCGPGTAPGPGAGVPLLTEDMQALTLRTLAASDVTKHYELVRELG
KGTYGKVDLVVYKGTGTKMALKFVNKSKTKLKNFLREVSITNSLSSSPFIIKVFDVVFET
EDCYVFAQEYAPAGDLFDIIPPQVGLPEDTVKRCVQQLGLALDFMHGRQLVHRDIKPENV
LLFDRECRRVKLADFGMTRRVGCRVKRVSGTIPYTAPEVCQAGRADGLAVDTGVDVWAFG
VLIFCVLTGNFPWEAASGADAFFEEFVRWQRGRLPGLPSQWRRFTEPALRMFQRLLALEP
ERRGPAKEVFRFLKHELTSELRRRPSHRARKPPGDRPPAAGPLRLEAPGPLKRTVLTESG
SGSRPAPPAVGSVPLPVPVPVPVPVPVPVPEPGLAPQGPPGRTDGRADKSKGQVVLATAI
EICV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0744 Download

>pFN21AE0744  5977 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCGTGGGCTGCCCAGAGCCTGAGCCGCCCCG
CTCCCTGACCTGCTGTGGGCCGGGGACTGCCCCTGGGCCTGGTGCCGGTGTGCCCCTTCT
CACTGAAGACATGCAGGCCCTGACTCTCCGCACACTGGCCGCCAGCGACGTCACCAAGCA
CTACGAACTAGTCCGGGAGCTGGGCAAAGGCACCTATGGGAAGGTTGACCTGGTGGTCTA
CAAGGGCACAGGCACAAAAATGGCACTGAAGTTTGTGAACAAGAGCAAAACCAAGCTGAA
GAACTTCCTACGGGAGGTGAGCATCACCAACAGCCTCTCCTCCAGCCCCTTCATCATCAA
GGTCTTTGACGTGGTCTTTGAGACAGAGGACTGCTACGTCTTTGCCCAGGAGTACGCACC
TGCTGGGGACCTGTTTGACATCATCCCTCCCCAGGTGGGGCTCCCTGAGGACACGGTGAA
GCGCTGTGTGCAGCAGCTGGGCCTGGCGCTGGACTTCATGCACGGGCGGCAGCTGGTGCA
CCGCGACATCAAGCCCGAGAACGTGCTGCTGTTCGACCGCGAGTGCCGCCGCGTAAAGCT
GGCCGACTTCGGCATGACGCGCCGCGTGGGCTGCCGCGTCAAGCGCGTGAGCGGCACCAT
CCCTTACACGGCGCCTGAGGTGTGCCAGGCGGGCCGCGCCGACGGGCTGGCGGTGGACAC
GGGCGTGGACGTGTGGGCCTTCGGCGTGCTCATCTTCTGCGTGCTCACCGGCAACTTCCC
GTGGGAGGCGGCGTCGGGCGCCGACGCCTTCTTCGAGGAGTTCGTGCGCTGGCAGCGGGG
CCGCCTGCCGGGGCTGCCTTCGCAGTGGCGCCGCTTCACCGAGCCCGCGCTGCGCATGTT
CCAGCGCTTACTGGCCCTGGAGCCCGAGCGCCGCGGCCCAGCCAAGGAGGTGTTCCGCTT
CCTCAAGCACGAGCTCACGTCCGAGCTGCGCCGCCGGCCCTCGCACCGCGCGCGCAAGCC
CCCCGGGGACCGCCCGCCCGCCGCCGGGCCACTGCGCCTCGAGGCGCCTGGGCCGCTCAA
GCGGACGGTGCTGACCGAGAGCGGCAGCGGCTCCCGGCCCGCGCCCCCCGCCGTCGGGTC
GGTGCCCTTGCCCGTGCCGGTGCCGGTGCCAGTGCCCGTGCCGGTGCCTGTGCCCGAGCC
CGGCCTAGCTCCCCAGGGGCCCCCCGGCCGGACCGACGGCCGCGCGGACAAGAGCAAAGG
GCAGGTGGTGCTGGCCACGGCCATCGAGATCTGCGTCGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT