(Linear) (Six-base) MAPSORT of: KIEE0744.seq Check: 6190 from: 1 to: 1272 KIEE0744.orf With 182 enzymes: SgfI * March 22, 2012 15:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 402 1227 1272 Size: 402 825 45 Fragments arranged by size: 825 402 45 AatII G_ACGT'C Cuts at: 0 146 1272 Size: 146 1126 AccI GT'mk_AC Cuts at: 0 212 1272 Size: 212 1060 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 262 292 1272 Size: 119 143 30 980 Fragments arranged by size: 980 143 119 30 AfeI AGC'GCT Cuts at: 0 457 880 1272 Size: 457 423 392 Fragments arranged by size: 457 423 392 AflIII A'CryG_T Cuts at: 0 704 1272 Size: 704 568 AleI CACnn'nnGTG Cuts at: 0 697 1272 Size: 697 575 AlwNI CAG_nnn'CTG Cuts at: 0 22 114 471 1272 Size: 22 92 357 801 Fragments arranged by size: 801 357 92 22 ApaI G_GGCC'C Cuts at: 0 1196 1272 Size: 1196 76 ApaLI G'TGCA_C Cuts at: 0 511 1272 Size: 511 761 AvaI C'yCGr_G Cuts at: 0 529 898 997 1033 1168 1272 Size: 529 369 99 36 135 104 Fragments arranged by size: 529 369 135 104 99 36 BanI G'GyrC_C Cuts at: 0 76 184 628 645 772 842 1038 1116 Size: 76 108 444 17 127 70 196 78 Cuts at: 1116 1134 1140 1158 1272 Size: 18 6 18 114 Fragments arranged by size: 444 196 127 114 108 78 76 70 18 18 17 6 BanII G_rGCy'C Cuts at: 0 437 860 899 950 1175 1196 1272 Size: 437 423 39 51 225 21 76 Fragments arranged by size: 437 423 225 76 51 39 21 BbeI G_GCGC'C Cuts at: 0 649 776 846 1042 1272 Size: 649 127 70 196 230 Fragments arranged by size: 649 230 196 127 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 107 1272 Size: 107 1165 BglI GCCn_nnn'nGGC Cuts at: 0 602 665 1272 Size: 602 63 607 Fragments arranged by size: 607 602 63 BglII A'GATC_T Cuts at: 0 1262 1272 Size: 1262 10 Bme1580I G_kGCm'C Cuts at: 0 89 223 515 1121 1151 1169 1196 1272 Size: 89 134 292 606 30 18 27 76 Fragments arranged by size: 606 292 134 89 76 30 27 18 BmgBI CAC'GTC Cuts at: 0 345 705 953 1272 Size: 345 360 248 319 Fragments arranged by size: 360 345 319 248 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 913 1272 Size: 300 613 359 Fragments arranged by size: 613 359 300 Bpu10I CC'TnA_GC Cuts at: 0 23 1272 Size: 23 1249 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 922 1036 1272 Size: 922 114 236 Fragments arranged by size: 922 236 114 BsaHI Gr'CG_yC Cuts at: 0 143 646 766 773 779 843 1039 1272 Size: 143 503 120 7 6 64 196 233 Fragments arranged by size: 503 233 196 143 120 64 7 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 295 325 751 781 1272 Size: 295 30 426 30 491 Fragments arranged by size: 491 426 295 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 303 805 1272 Size: 303 502 467 Fragments arranged by size: 502 467 303 BseYI C'CCAG_C Cuts at: 0 174 397 471 912 1272 Size: 174 223 74 441 360 Fragments arranged by size: 441 360 223 174 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1272 Size: 484 788 BsiEI CG_ry'CG Cuts at: 0 552 1203 1215 1272 Size: 552 651 12 57 Fragments arranged by size: 651 552 57 12 BsiHKAI G_wGCw'C Cuts at: 0 515 725 740 950 1272 Size: 515 210 15 210 322 Fragments arranged by size: 515 322 210 210 15 Bsp1286I G_dGCh'C Cuts at: 0 89 223 437 515 725 740 860 899 Size: 89 134 214 78 210 15 120 39 Cuts at: 899 950 1121 1151 1169 1175 1196 1272 Size: 51 171 30 18 6 21 76 Fragments arranged by size: 214 210 171 134 120 89 78 76 51 39 30 21 18 15 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 52 402 1227 1272 Size: 52 350 825 45 Fragments arranged by size: 825 350 52 45 BsrBI CCG'CTC Cuts at: 0 35 626 1050 1076 1272 Size: 35 591 424 26 196 Fragments arranged by size: 591 424 196 35 26 BsrFI r'CCGG_y Cuts at: 0 79 742 968 1131 1137 1155 1272 Size: 79 663 226 163 6 18 117 Fragments arranged by size: 663 226 163 117 79 18 6 BssHII G'CGCG_C Cuts at: 0 983 985 1272 Size: 983 2 287 Fragments arranged by size: 983 287 2 BssSI C'ACGA_G Cuts at: 0 943 1272 Size: 943 329 BstAPI GCAn_nnn'nTGC Cuts at: 0 510 1242 1272 Size: 510 732 30 Fragments arranged by size: 732 510 30 BstYI r'GATC_y Cuts at: 0 1262 1272 Size: 1262 10 Bsu36I CC'TnA_GG Cuts at: 0 440 650 1272 Size: 440 210 622 Fragments arranged by size: 622 440 210 BtgI C'CryG_G Cuts at: 0 754 906 1250 1272 Size: 754 152 344 22 Fragments arranged by size: 754 344 152 22 BtsI GCAGTG_nn' Cuts at: 0 844 1024 1272 Size: 844 180 248 Fragments arranged by size: 844 248 180 DrdI GACnn_nn'nnGTC Cuts at: 0 208 370 1272 Size: 208 162 902 Fragments arranged by size: 902 208 162 EaeI y'GGCC_r Cuts at: 0 131 575 1200 1212 1247 1253 1272 Size: 131 444 625 12 35 6 19 Fragments arranged by size: 625 444 131 35 19 12 6 EagI C'GGCC_G Cuts at: 0 1200 1212 1272 Size: 1200 12 60 Fragments arranged by size: 1200 60 12 EcoICRI GAG'CTC Cuts at: 0 948 1272 Size: 948 324 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 262 292 300 913 1272 Size: 119 143 30 8 613 359 Fragments arranged by size: 613 359 143 119 30 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 286 1272 Size: 286 986 EcoO109I rG'GnC_Cy Cuts at: 0 111 402 1192 1193 1272 Size: 111 291 790 1 79 Fragments arranged by size: 790 291 111 79 1 FspI TGC'GCA Cuts at: 0 868 1272 Size: 868 404 HaeII r_GCGC'y Cuts at: 0 459 484 649 776 846 882 906 1042 Size: 459 25 165 127 70 36 24 136 Cuts at: 1042 1272 Size: 230 Fragments arranged by size: 459 230 165 136 127 70 36 25 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 751 783 937 969 1272 Size: 751 32 154 32 303 Fragments arranged by size: 751 303 154 32 32 HincII GTy'rAC Cuts at: 0 201 1272 Size: 201 1071 KasI G'GCGC_C Cuts at: 0 645 772 842 1038 1272 Size: 645 127 70 196 234 Fragments arranged by size: 645 234 196 127 70 MscI TGG'CCA Cuts at: 0 1249 1272 Size: 1249 23 MslI CAynn'nnrTG Cuts at: 0 232 697 1272 Size: 232 465 575 Fragments arranged by size: 575 465 232 MspA1I CmG'CkG Cuts at: 0 471 507 810 908 1082 1272 Size: 471 36 303 98 174 190 Fragments arranged by size: 471 303 190 174 98 36 NaeI GCC'GGC Cuts at: 0 970 1272 Size: 970 302 NarI GG'CG_CC Cuts at: 0 646 773 843 1039 1272 Size: 646 127 70 196 233 Fragments arranged by size: 646 233 196 127 70 NgoMIV G'CCGG_C Cuts at: 0 968 1272 Size: 968 304 NspI r_CATG'y Cuts at: 0 108 873 1272 Size: 108 765 399 Fragments arranged by size: 765 399 108 PasI CC'CwG_GG Cuts at: 0 67 1188 1272 Size: 67 1121 84 Fragments arranged by size: 1121 84 67 PfoI T'CCnGG_A Cuts at: 0 167 1272 Size: 167 1105 PpuMI rG'GwC_Cy Cuts at: 0 402 1272 Size: 402 870 PspOMI G'GGCC_C Cuts at: 0 1192 1272 Size: 1192 80 PspXI vC'TCGA_Gb Cuts at: 0 1033 1272 Size: 1033 239 PvuII CAG'CTG Cuts at: 0 471 507 1272 Size: 471 36 765 Fragments arranged by size: 765 471 36 RsrII CG'GwC_CG Cuts at: 0 1205 1272 Size: 1205 67 SacI G_AGCT'C Cuts at: 0 950 1272 Size: 950 322 SacII CC_GC'GG Cuts at: 0 909 1272 Size: 909 363 SexAI A'CCwGG_T Cuts at: 0 203 1272 Size: 203 1069 SfoI GGC'GCC Cuts at: 0 647 774 844 1040 1272 Size: 647 127 70 196 232 Fragments arranged by size: 647 232 196 127 70 SmaI CCC'GGG Cuts at: 0 999 1272 Size: 999 273 SmlI C'TyrA_G Cuts at: 0 937 1033 1051 1272 Size: 937 96 18 221 Fragments arranged by size: 937 221 96 18 SpeI A'CTAG_T Cuts at: 0 162 1272 Size: 162 1110 StyI C'CwwG_G Cuts at: 0 917 1272 Size: 917 355 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1084 1222 1272 Size: 1084 138 50 Fragments arranged by size: 1084 138 50 Tth111I GACn'n_nGTC Cuts at: 0 346 1272 Size: 346 926 XhoI C'TCGA_G Cuts at: 0 1033 1272 Size: 1033 239 XmaI C'CCGG_G Cuts at: 0 997 1272 Size: 997 275 XmnI GAAnn'nnTTC Cuts at: 0 277 1272 Size: 277 995 ZraI GAC'GTC Cuts at: 0 144 1272 Size: 144 1128 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SexAI SfoI SmaI SmlI SpeI StyI TaqII Tth111I XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EarI EciI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI