Restriction map for
pFN21AE0744
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI AlwI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI DraI DraIII
Eam1105I EarI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI SalI SapI SbfI
ScaI SfcI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 142 GACGTC GACGT^C
AccI 211 GTCTAC GT^[AC][GT]AC
AfaI 387 GTAC GT^AC
AflIII 704 ACGTGT A^C[GA][CT]GT
Alw44I 511 GTGCAC G^TGCAC
ApaI 1192 GGGCCC GGGCC^C
ApaLI 511 GTGCAC G^TGCAC
Asp700I 273 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 343 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 866 TGCGCA TGC^GCA
BalI 1247 TGGCCA TGG^CCA
BbsI 100 GAAGAC GAAGAC(2/6)
BglII 1262 AGATCT A^GATCT
BpuAI 100 GAAGAC GAAGAC(2/6)
BsgI 463 GTGCAG GTGCAG(16/14)
BsmAI 355 GAGAC GTCTC(1/5)
BssSI 943 CACGAG CACGAG(-5/-1)
BstYI 1262 AGATCT [GA]^GATC[CT]
CpoI 1204 CGGACCG CG^G[AT]CCG
DpnII 1263 GATC ^GATC
EcoNI 282 CCTACGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 917 CCAAGG C^C[AT][AT]GG
FspI 866 TGCGCA TGC^GCA
HincII 199 GTTGAC GT[CT]^[GA]AC
HindII 199 GTTGAC GT[CT]^[GA]AC
HinfI 117 GACTC G^A[ACGT]TC
KspI 906 CCGCGG CCGC^GG
MaeIII 145 GTCAC ^GT[ACGT]AC
MboI 1263 GATC ^GATC
MflI 1262 AGATCT [GA]^GATC[CT]
MluNI 1247 TGGCCA TGG^CCA
MscI 1247 TGGCCA TGG^CCA
NaeI 968 GCCGGC GCC^GGC
NdeII 1263 GATC ^GATC
NgoMIV 968 GCCGGC G^CCGGC
PaeR7I 1033 CTCGAG C^TCGAG
PflFI 343 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 117 GACTC GAGTC(4/5)
PpuMI 401 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 387 GTAC GT^AC
RsrII 1204 CGGACCG CG^G[AT]CCG
SacI 946 GAGCTC GAGCT^C
SacII 906 CCGCGG CCGC^GG
Sau3AI 1263 GATC ^GATC
SexAI 203 ACCTGGT A^CC[AT]GGT
SfaNI 296 GCATC GCATC(5/9)
SmaI 997 CCCGGG CCC^GGG
SpeI 162 ACTAGT A^CTAGT
StyI 917 CCAAGG C^C[AT][AT]GG
Tsp45I 145 GTCAC ^GT[GC]AC
Tth111I 343 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1033 CTCGAG C^TCGAG
XhoII 1262 AGATCT [GA]^GATC[CT]
XmaCI 997 CCCGGG C^CCGGG
XmaI 997 CCCGGG C^CCGGG
XmnI 273 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AocI 439 CCTGAGG CC^T[ACGT]AGG
649 CCTGAGG
Aor51HI 455 AGCGCT AGC^GCT
878 AGCGCT
BfaI 163 CTAG C^TAG
1180 CTAG
BglI 596 GCCGCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
659 GCCAGGCGGGC
BpmI 315 CTCCAG CTGGAG(16/14)
892 CTGGAG
BseRI 312 CTCCTC GAGGAG(10/8)
790 GAGGAG
BssHII 983 GCGCGC G^CGCGC
985 GCGCGC
BstAPI 504 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1236 GCAGGTGGTGC
BstF5I 417 CATCC GGATG(2/0)
633 CATCC
Bsu36I 439 CCTGAGG CC^T[ACGT]AGG
649 CCTGAGG
DrdI 202 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
364 GACTGCTACGTC
EagI 1200 CGGCCG C^GGCCG
1212 CGGCCG
EclXI 1200 CGGCCG C^GGCCG
1212 CGGCCG
Eco47III 455 AGCGCT AGC^GCT
878 AGCGCT
Eco52I 1200 CGGCCG C^GGCCG
1212 CGGCCG
Eco81I 439 CCTGAGG CC^T[ACGT]AGG
649 CCTGAGG
FokI 417 CATCC GGATG(9/13)
633 CATCC
MaeI 163 CTAG C^TAG
1180 CTAG
MslI 228 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
693 CACGGGCGTG
NspI 104 ACATGC [GA]CATG^[CT]
869 GCATGT
PvuII 469 CAGCTG CAG^CTG
505 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
Sau96I ScrFI SfoI SmlI TaqI
TseI TspRI TthHB8I