ALG9 ORF clone pFN21AE0327

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Halotag ORF Clone pFN21AE0327

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Clone info.

Species

human

Clone Name

pFN21AE0327 Sequence (with vector) Restriction Map

Product ID

FHC04085

Flexi Clone PID

FXC04085

ORF Sequence

DNA sequence (1854 bp)
Protein sequence (618 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527104]

Gene

KIEE0327

Symbol

ALG9

Alias : CDG1L, DIBD1, GIKANIS, LOH11CR1J

Description

ALG9, alpha-1,2-mannosyltransferase, transcript variant 1

Original Clone ID

oc01027

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1854 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 618 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS73380.1 0 100.0 ALG9 CCDS73379.1 0 98.9 ALG9 CCDS41714.1 2.7e-202 100.0 ALG9 CCDS53709.1 3.3e-197 98.4 ALG9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005599 61 489 PF03901 GPI mannosyltransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 132 QTNKILVFYFLRCLLAFVSCICE 154 PRIMARY 23 2 172 MMLAFLVLSTGMFCSSSAFLPS 193 SECONDARY 22 3 218 GVAAGAILGWPFSAALGLPIAFD 240 PRIMARY 23 4 260 ILFLVPVVVIDSYYYGKLVIAPL 282 PRIMARY 23 5 307 LINGFLNFNVAFALALLVLPLTS 329 PRIMARY 23 6 343 LGHPYWLTLAPMYIWFIIFFIQ 364 SECONDARY 22 7 372 LFPVYPLICLCGAVALSALQHSF 394 PRIMARY 23 8 416 TSNWLALGTVFLFGLLSFSRSVA 438 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_079016 618 263210,606941,608776 100.0 100.0 XP_005277780 629 263210,606941,608776 100.0 99.8 NP_001071158 611 263210,606941,608776 98.9 100.0 XP_005277781 622 263210,606941,608776 98.9 99.8 XP_011541294 618 263210,606941,608776 99.1 94.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0327 Download

>KIEE0327  1854 bp
ATGGCTAGTCGAGGGGCTCGGCAGCGCCTGAAGGGCAGCGGGGCCAGCAGTGGGGATACG
GCCCCGGCTGCGGACAAGCTGCGGGAGCTGCTGGGCAGCCGAGAGGCGGGCGGCGCGGAG
CACCGGACCGAGTTATCTGGGAACAAAGCAGGACAAGTCTGGGCACCTGAAGGATCTACT
GCTTTCAAGTGTCTGCTTTCAGCAAGGTTATGTGCTGCTCTCCTGAGCAACATCTCTGAC
TGTGATGAAACATTCAACTACTGGGAGCCAACACACTACCTCATCTATGGGGAAGGGTTT
CAGACTTGGGAATATTCCCCAGCATATGCCATTCGCTCCTATGCTTACCTGTTGCTTCAT
GCCTGGCCAGCTGCATTTCATGCAAGAATTCTACAAACTAATAAGATTCTTGTGTTTTAC
TTTTTGCGATGTCTTCTGGCTTTTGTGAGCTGTATTTGTGAACTTTACTTTTACAAGGCT
GTGTGCAAGAAGTTTGGGTTGCACGTGAGTCGAATGATGCTAGCCTTCTTGGTTCTCAGC
ACTGGCATGTTTTGCTCATCATCAGCATTCCTTCCTAGTAGCTTCTGTATGTACACTACG
TTGATAGCCATGACTGGATGGTATATGGACAAGACTTCCATTGCTGTGCTGGGAGTAGCA
GCTGGGGCTATCTTAGGCTGGCCATTCAGTGCAGCTCTTGGTTTACCCATTGCCTTTGAT
TTGCTGGTCATGAAACACAGGTGGAAGAGTTTCTTTCATTGGTCGCTGATGGCCCTCATA
CTATTTCTGGTGCCTGTGGTGGTCATTGACAGCTACTATTATGGGAAGTTGGTGATTGCA
CCACTCAACATTGTTTTGTATAATGTCTTTACTCCTCATGGACCTGATCTTTATGGTACA
GAACCCTGGTATTTCTATTTAATTAATGGATTTCTGAATTTCAATGTAGCCTTTGCTTTG
GCTCTCCTAGTCCTACCACTGACTTCTCTTATGGAATACCTGCTGCAGAGATTTCATGTT
CAGAATTTAGGCCACCCGTATTGGCTTACCTTGGCTCCAATGTATATTTGGTTTATAATT
TTCTTCATCCAGCCTCACAAAGAGGAGAGATTTCTTTTCCCTGTGTATCCACTTATATGT
CTCTGTGGCGCTGTGGCTCTCTCTGCACTTCAGCACAGTTTTCTGTACTTCCAGAAATGT
TACCACTTTGTGTTTCAACGATATCGCCTGGAGCACTATACTGTGACATCGAATTGGCTG
GCATTAGGAACTGTCTTCCTGTTTGGGCTCTTGTCATTTTCTCGCTCTGTGGCACTGTTC
AGAGGATATCACGGGCCCCTTGATTTGTATCCAGAATTTTACCGAATTGCTACAGACCCA
ACCATCCACACTGTCCCAGAAGGCAGACCTGTGAATGTCTGTGTGGGAAAAGAGTGGTAT
CGATTTCCCAGCAGCTTCCTTCTTCCTGACAATTGGCAGCTTCAGTTCATTCCATCAGAG
TTCAGAGGTCAGTTACCAAAACCTTTTGCAGAAGGACCTCTGGCCACCCGGATTGTTCCT
ACTGACATGAATGACCAGAATCTAGAAGAGCCATCCAGATATATTGATATCAGTAAATGC
CATTATTTAGTGGATTTGGACACCATGAGAGAAACACCCCGGGAGCCAAAATATTCATCC
AATAAAGAAGAATGGATCAGCTTGGCCTATAGACCATTCCTTGATGCTTCTAGATCTTCA
AAGCTGCTGCGGGCATTCTATGTCCCCTTCCTGTCAGATCAGTATACAGTGTACGTAAAC
TACACCATCCTCAAACCCCGGAAAGCAAAGCAAATCAGGAAGAAAAGTGGAGGT

ORF Protein

Cloned ORF protein sequence for pFN21AE0327 Download

>KIEE0327  618 aa
MASRGARQRLKGSGASSGDTAPAADKLRELLGSREAGGAEHRTELSGNKAGQVWAPEGST
AFKCLLSARLCAALLSNISDCDETFNYWEPTHYLIYGEGFQTWEYSPAYAIRSYAYLLLH
AWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKAVCKKFGLHVSRMMLAFLVLS
TGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLGVAAGAILGWPFSAALGLPIAFD
LLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIAPLNIVLYNVFTPHGPDLYGT
EPWYFYLINGFLNFNVAFALALLVLPLTSLMEYLLQRFHVQNLGHPYWLTLAPMYIWFII
FFIQPHKEERFLFPVYPLICLCGAVALSALQHSFLYFQKCYHFVFQRYRLEHYTVTSNWL
ALGTVFLFGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPEGRPVNVCVGKEWY
RFPSSFLLPDNWQLQFIPSEFRGQLPKPFAEGPLATRIVPTDMNDQNLEEPSRYIDISKC
HYLVDLDTMRETPREPKYSSNKEEWISLAYRPFLDASRSSKLLRAFYVPFLSDQYTVYVN
YTILKPRKAKQIRKKSGG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0327 Download

>pFN21AE0327  6559 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTAGTCGAGGGGCTCGGCAGCGCCTGAAGGG
CAGCGGGGCCAGCAGTGGGGATACGGCCCCGGCTGCGGACAAGCTGCGGGAGCTGCTGGG
CAGCCGAGAGGCGGGCGGCGCGGAGCACCGGACCGAGTTATCTGGGAACAAAGCAGGACA
AGTCTGGGCACCTGAAGGATCTACTGCTTTCAAGTGTCTGCTTTCAGCAAGGTTATGTGC
TGCTCTCCTGAGCAACATCTCTGACTGTGATGAAACATTCAACTACTGGGAGCCAACACA
CTACCTCATCTATGGGGAAGGGTTTCAGACTTGGGAATATTCCCCAGCATATGCCATTCG
CTCCTATGCTTACCTGTTGCTTCATGCCTGGCCAGCTGCATTTCATGCAAGAATTCTACA
AACTAATAAGATTCTTGTGTTTTACTTTTTGCGATGTCTTCTGGCTTTTGTGAGCTGTAT
TTGTGAACTTTACTTTTACAAGGCTGTGTGCAAGAAGTTTGGGTTGCACGTGAGTCGAAT
GATGCTAGCCTTCTTGGTTCTCAGCACTGGCATGTTTTGCTCATCATCAGCATTCCTTCC
TAGTAGCTTCTGTATGTACACTACGTTGATAGCCATGACTGGATGGTATATGGACAAGAC
TTCCATTGCTGTGCTGGGAGTAGCAGCTGGGGCTATCTTAGGCTGGCCATTCAGTGCAGC
TCTTGGTTTACCCATTGCCTTTGATTTGCTGGTCATGAAACACAGGTGGAAGAGTTTCTT
TCATTGGTCGCTGATGGCCCTCATACTATTTCTGGTGCCTGTGGTGGTCATTGACAGCTA
CTATTATGGGAAGTTGGTGATTGCACCACTCAACATTGTTTTGTATAATGTCTTTACTCC
TCATGGACCTGATCTTTATGGTACAGAACCCTGGTATTTCTATTTAATTAATGGATTTCT
GAATTTCAATGTAGCCTTTGCTTTGGCTCTCCTAGTCCTACCACTGACTTCTCTTATGGA
ATACCTGCTGCAGAGATTTCATGTTCAGAATTTAGGCCACCCGTATTGGCTTACCTTGGC
TCCAATGTATATTTGGTTTATAATTTTCTTCATCCAGCCTCACAAAGAGGAGAGATTTCT
TTTCCCTGTGTATCCACTTATATGTCTCTGTGGCGCTGTGGCTCTCTCTGCACTTCAGCA
CAGTTTTCTGTACTTCCAGAAATGTTACCACTTTGTGTTTCAACGATATCGCCTGGAGCA
CTATACTGTGACATCGAATTGGCTGGCATTAGGAACTGTCTTCCTGTTTGGGCTCTTGTC
ATTTTCTCGCTCTGTGGCACTGTTCAGAGGATATCACGGGCCCCTTGATTTGTATCCAGA
ATTTTACCGAATTGCTACAGACCCAACCATCCACACTGTCCCAGAAGGCAGACCTGTGAA
TGTCTGTGTGGGAAAAGAGTGGTATCGATTTCCCAGCAGCTTCCTTCTTCCTGACAATTG
GCAGCTTCAGTTCATTCCATCAGAGTTCAGAGGTCAGTTACCAAAACCTTTTGCAGAAGG
ACCTCTGGCCACCCGGATTGTTCCTACTGACATGAATGACCAGAATCTAGAAGAGCCATC
CAGATATATTGATATCAGTAAATGCCATTATTTAGTGGATTTGGACACCATGAGAGAAAC
ACCCCGGGAGCCAAAATATTCATCCAATAAAGAAGAATGGATCAGCTTGGCCTATAGACC
ATTCCTTGATGCTTCTAGATCTTCAAAGCTGCTGCGGGCATTCTATGTCCCCTTCCTGTC
AGATCAGTATACAGTGTACGTAAACTACACCATCCTCAAACCCCGGAAAGCAAAGCAAAT
CAGGAAGAAAAGTGGAGGTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT