Restriction map for
pFN21AE0327
(Linear) (Six-base) MAPSORT of: KIEE0327.seq Check: 2910 from: 1 to: 1854
KIEE0327.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1783 1854
Size: 1783 71
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 188 1153 1465 1854
Size: 49 139 965 312 389
Fragments arranged by size:
965 389 312 139 49
AlwNI CAG_nnn'CTG
Cuts at: 0 27 1854
Size: 27 1827
ApaI G_GGCC'C
Cuts at: 0 1337 1854
Size: 1337 517
ApoI r'AATT_y
Cuts at: 0 386 936 1023 1354 1854
Size: 386 550 87 331 500
Fragments arranged by size:
550 500 386 331 87
AseI AT'TA_AT
Cuts at: 0 923 1854
Size: 923 931
AvaI C'yCGr_G
Cuts at: 0 1658 1854
Size: 1658 196
BanI G'GyrC_C
Cuts at: 0 162 789 1854
Size: 162 627 1065
Fragments arranged by size:
1065 627 162
BanII G_rGCy'C
Cuts at: 0 18 1289 1337 1854
Size: 18 1271 48 517
Fragments arranged by size:
1271 517 48 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 424 1266 1854
Size: 424 842 588
Fragments arranged by size:
842 588 424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 524 1854
Size: 490 34 1330
Fragments arranged by size:
1330 490 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 48 1136 1358 1854
Size: 48 1088 222 496
Fragments arranged by size:
1088 496 222 48
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1854
Size: 32 1822
BglII A'GATC_T
Cuts at: 0 1732 1854
Size: 1732 122
Bme1580I G_kGCm'C
Cuts at: 0 165 1337 1854
Size: 165 1172 517
Fragments arranged by size:
1172 517 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 1854
Size: 270 1584
BmtI G_CTAG'C
Cuts at: 0 523 1854
Size: 523 1331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1854
Size: 1249 605
Bpu10I CC'TnA_GC
Cuts at: 0 223 1854
Size: 223 1631
BsaAI yAC'GTr
Cuts at: 0 504 1794 1854
Size: 504 1290 60
Fragments arranged by size:
1290 504 60
BsaBI GATnn'nnATC
Cuts at: 0 1346 1854
Size: 1346 508
BsaWI w'CCGG_w
Cuts at: 0 122 1854
Size: 122 1732
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1038 1068 1854
Size: 1038 30 786
Fragments arranged by size:
1038 786 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 863 1117 1854
Size: 863 254 737
Fragments arranged by size:
863 737 254
BseYI C'CCAG_C
Cuts at: 0 90 318 648 661 1447 1854
Size: 90 228 330 13 786 407
Fragments arranged by size:
786 407 330 228 90 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1148 1854
Size: 710 438 706
Fragments arranged by size:
710 706 438
BsiHKAI G_wGCw'C
Cuts at: 0 122 1235 1854
Size: 122 1113 619
Fragments arranged by size:
1113 619 122
BsmI GAATG_Cn'
Cuts at: 0 565 1753 1854
Size: 565 1188 101
Fragments arranged by size:
1188 565 101
Bsp1286I G_dGCh'C
Cuts at: 0 18 122 165 1235 1289 1337 1854
Size: 18 104 43 1070 54 48 517
Fragments arranged by size:
1070 517 104 54 48 43 18
BspHI T'CATG_A
Cuts at: 0 728 1854
Size: 728 1126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1007 1854
Size: 1007 847
BsrDI GCAATG_nn'
Cuts at: 0 638 707 1854
Size: 638 69 1147
Fragments arranged by size:
1147 638 69
BsrGI T'GTAC_A
Cuts at: 0 590 1854
Size: 590 1264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 379 551 1854
Size: 379 172 1303
Fragments arranged by size:
1303 379 172
BstYI r'GATC_y
Cuts at: 0 172 1732 1854
Size: 172 1560 122
Fragments arranged by size:
1560 172 122
BstZ17I GTA'TAC
Cuts at: 0 1784 1854
Size: 1784 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 441 1854
Size: 441 1413
BtsI GCAGTG_nn'
Cuts at: 0 54 1854
Size: 54 1800
ClaI AT'CG_AT
Cuts at: 0 1440 1854
Size: 1440 414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1021 1056 1533 1568 1854
Size: 1021 35 477 35 286
Fragments arranged by size:
1021 477 286 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1209 1854
Size: 1209 645
EaeI y'GGCC_r
Cuts at: 0 364 679 1541 1854
Size: 364 315 862 313
Fragments arranged by size:
862 364 315 313
EarI CTCTTCn'nnn_
Cuts at: 0 739 1580 1854
Size: 739 841 274
Fragments arranged by size:
841 739 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 188 1153 1249 1465 1854
Size: 49 139 965 96 216 389
Fragments arranged by size:
965 389 216 139 96 49
EcoO109I rG'GnC_Cy
Cuts at: 0 1334 1534 1854
Size: 1334 200 320
Fragments arranged by size:
1334 320 200
EcoRI G'AATT_C
Cuts at: 0 386 1854
Size: 386 1468
EcoRV GAT'ATC
Cuts at: 0 1222 1327 1608 1854
Size: 1222 105 281 246
Fragments arranged by size:
1222 281 246 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 768 1854
Size: 768 1086
HaeII r_GCGC'y
Cuts at: 0 27 1151 1854
Size: 27 1124 703
Fragments arranged by size:
1124 703 27
MfeI C'AATT_G
Cuts at: 0 1470 1854
Size: 1470 384
MscI TGG'CCA
Cuts at: 0 366 681 1543 1854
Size: 366 315 862 311
Fragments arranged by size:
862 366 315 311
MslI CAynn'nnrTG
Cuts at: 0 544 643 1134 1854
Size: 544 99 491 720
Fragments arranged by size:
720 544 491 99
MspA1I CmG'CkG
Cuts at: 0 38 370 661 1854
Size: 38 332 291 1193
Fragments arranged by size:
1193 332 291 38
NdeI CA'TA_TG
Cuts at: 0 324 1854
Size: 324 1530
NheI G'CTAG_C
Cuts at: 0 519 1854
Size: 519 1335
NspI r_CATG'y
Cuts at: 0 549 1854
Size: 549 1305
PacI TTA_AT'TAA
Cuts at: 0 923 1854
Size: 923 931
PmlI CAC'GTG
Cuts at: 0 504 1854
Size: 504 1350
PpuMI rG'GwC_Cy
Cuts at: 0 1534 1854
Size: 1534 320
PsiI TTA'TAA
Cuts at: 0 1075 1854
Size: 1075 779
PspOMI G'GGCC_C
Cuts at: 0 1333 1854
Size: 1333 521
PstI C_TGCA'G
Cuts at: 0 1007 1854
Size: 1007 847
PvuII CAG'CTG
Cuts at: 0 370 661 1854
Size: 370 291 1193
Fragments arranged by size:
1193 370 291
RsrII CG'GwC_CG
Cuts at: 0 125 1854
Size: 125 1729
SapI GCTCTTCn'nnn_
Cuts at: 0 1580 1854
Size: 1580 274
SfcI C'TryA_G
Cuts at: 0 1003 1370 1707 1854
Size: 1003 367 337 147
Fragments arranged by size:
1003 367 337 147
SmaI CCC'GGG
Cuts at: 0 1660 1854
Size: 1660 194
SnaBI TAC'GTA
Cuts at: 0 1794 1854
Size: 1794 60
SspI AAT'ATT
Cuts at: 0 313 1672 1854
Size: 313 1359 182
Fragments arranged by size:
1359 313 182
StyI C'CwwG_G
Cuts at: 0 1049 1854
Size: 1049 805
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 1854
Size: 142 1712
TatI w'GTAC_w
Cuts at: 0 590 1184 1854
Size: 590 594 670
Fragments arranged by size:
670 594 590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1830 1854
Size: 1830 24
XbaI T'CTAG_A
Cuts at: 0 1581 1729 1854
Size: 1581 148 125
Fragments arranged by size:
1581 148 125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 645 1064 1854
Size: 645 419 790
Fragments arranged by size:
790 645 419
XmaI C'CCGG_G
Cuts at: 0 1658 1854
Size: 1658 196
XmnI GAAnn'nnTTC
Cuts at: 0 296 748 1854
Size: 296 452 1106
Fragments arranged by size:
1106 452 296
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AseI AvaI BanI
BanII BbsI BcgI BciVI BglI BglII Bme1580I BmrI
BmtI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII MfeI MscI MslI MspA1I NdeI NheI NspI
PacI PmlI PpuMI PsiI PspOMI PstI PvuII RsrII
SapI SfcI SmaI SnaBI SspI StyI TaqII TatI
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AsiSI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BlpI BmgBI
BplI BpuEI BsaI BsaHI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI Bsu36I
BtgI DraI DrdI EagI EciI EcoICRI EcoNI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NcoI NgoMIV NotI NruI
NsiI PasI PciI PflMI PfoI PmeI PpiI PshAI
PspXI PsrI PvuI SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SpeI
SphI SrfI StuI SwaI Tth111I XhoI ZraI