Restriction map for
pFN21AE0327
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstXI Bsu36I CelII
Cfr10I DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MluI MroI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PaeR7I PflFI PflMI PinAI PmeI
PshAI Psp1406I PvuI SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1782 GTATAC GT^[AC][GT]AC
AccII 114 CGCG CG^CG
AlwNI 22 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1333 GGGCCC GGGCC^C
AseI 922 ATTAAT AT^TAAT
AsnI 922 ATTAAT AT^TAAT
AvaI 1658 CCCGGG C^[CT]CG[GA]G
BbrPI 502 CACGTG CAC^GTG
BcgI 501 GCACGTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 26 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1732 AGATCT A^GATCT
BpmI 1228 CTGGAG CTGGAG(16/14)
BsaBI 1342 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 122 ACCGGA [AT]^CCGG[AT]
BsmAI 1139 GTCTC GTCTC(1/5)
BsoBI 1658 CCCGGG C^[CT]CG[GA]G
Bsp1407I 590 TGTACA T^GTACA
BspDI 1439 ATCGAT AT^CGAT
BspHI 728 TCATGA T^CATGA
BspMI 998 ACCTGC ACCTGC(4/8)
BsrGI 590 TGTACA T^GTACA
Bst1107I 1782 GTATAC GTA^TAC
BstUI 114 CGCG CG^CG
BstZ17I 1782 GTATAC GTA^TAC
ClaI 1439 ATCGAT AT^CGAT
CpoI 124 CGGACCG CG^G[AT]CCG
DraIII 1204 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 386 GAATTC G^AATTC
EcoT14I 1049 CCTTGG C^C[AT][AT]GG
HphI 831 GGTGA GGTGA(8/7)
MamI 1342 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1470 CAATTG C^AATTG
MunI 1470 CAATTG C^AATTG
MvnI 114 CGCG CG^CG
NdeI 323 CATATG CA^TATG
NheI 519 GCTAGC G^CTAGC
NspI 545 GCATGT [GA]CATG^[CT]
PacI 919 TTAATTAA TTAAT^TAA
PleI 507 GAGTC GAGTC(4/5)
PmaCI 502 CACGTG CAC^GTG
PmlI 502 CACGTG CAC^GTG
PpuMI 1533 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 922 ATTAAT AT^TAAT
PstI 1003 CTGCAG CTGCA^G
RcaI 728 TCATGA T^CATGA
RsrII 124 CGGACCG CG^G[AT]CCG
SapI 1585 GAAGAGC GCTCTTC(1/4)
SmaI 1658 CCCGGG CCC^GGG
SnaBI 1792 TACGTA TAC^GTA
SspBI 590 TGTACA T^GTACA
StyI 1049 CCTTGG C^C[AT][AT]GG
Tsp45I 1243 GTGAC ^GT[GC]AC
XmaCI 1658 CCCGGG C^CCGGG
XmaI 1658 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 172 GGATC GGATC(4/5)
1694 GGATC
Asp700I 292 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
744 GAAGAGTTTC
AspHI 118 GAGCAC G[AT]GC[AT]^C
1231 GAGCAC
BanI 162 GGCACC G^G[CT][GA]CC
789 GGTGCC
BbsI 431 GTCTTC GAAGAC(2/6)
1273 GTCTTC
BpuAI 431 GTCTTC GAAGAC(2/6)
1273 GTCTTC
BsaAI 502 CACGTG [CT]AC^GT[GA]
1792 TACGTA
BseRI 872 CTCCTC GAGGAG(10/8)
1102 GAGGAG
BsgI 1163 CTGCAC GTGCAG(16/14)
689 GTGCAG
BsiHKAI 118 GAGCAC G[AT]GC[AT]^C
1231 GAGCAC
BsmFI 1393 GTCCC GGGAC(10/14)
1762 GTCCC
BsmI 565 GCATTC GAATGC(1/-1)
1753 GCATTC
BsrDI 639 CATTGC GCAATG(2/0)
708 CATTGC
BstAPI 373 GCATTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
545 GCATGTTTTGC
BstYI 172 GGATCT [GA]^GATC[CT]
1732 AGATCT
DraII 1333 GGGCCCC [GA]G^G[ACGT]CC[CT]
1533 AGGACCT
EarI 744 GAAGAG CTCTTC(1/4)
1585 GAAGAG
EcoO109I 1333 GGGCCCC [GA]G^G[ACGT]CC[CT]
1533 AGGACCT
HaeII 23 AGCGCC [GA]GCGC^[CT]
1147 GGCGCT
Ksp632I 744 GAAGAG CTCTTC(1/4)
1585 GAAGAG
MflI 172 GGATCT [GA]^GATC[CT]
1732 AGATCT
MseI 919 TTAA T^TAA
923 TTAA
PvuII 368 CAGCTG CAG^CTG
659 CAGCTG
SfaNI 516 GATGC GCATC(5/9)
1723 GATGC
SspI 311 AATATT AAT^ATT
1670 AATATT
TfiI 405 GATTC G^A[AT]TC
1578 GAATC
Tru9I 919 TTAA T^TAA
923 TTAA
XbaI 1581 TCTAGA T^CTAGA
1729 TCTAGA
XcmI 638 CCATTGCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1057 CCAATGTATATTTGG
XhoII 172 GGATCT [GA]^GATC[CT]
1732 AGATCT
XmnI 292 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
744 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BalI BanII BbvI BciVI
BcnI BfaI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoRV FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI TaqI TseI Tsp509I TspRI
TthHB8I