CARD9 ORF clone pFN21AB9953

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Halotag ORF Clone pFN21AB9953

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Clone info.

Species

human

Clone Name

pFN21AB9953 Sequence (with vector) Restriction Map

Product ID

FHC03531

Flexi Clone PID

FXC03531

ORF Sequence

DNA sequence (1608 bp)
Protein sequence (536 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527615]

Gene

KIBB9953

Symbol

CARD9

Alias : CANDF2, hCARD9

Description

caspase recruitment domain family, member 9, transcript variant 1

Original Clone ID

cs01697

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1608 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 536 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6997.1 3.9e-110 99.8 CARD9 CCDS48057.1 4.2e-98 99.0 CARD9 CCDS5336.2 2.6e-24 39.9 CARD11 CCDS13948.1 4.1e-17 35.0 CARD10 CCDS58605.1 1.9e-14 31.9 CARD14 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001315 11 96 PF00619 Caspase Recruitment ProfileScan IPR001315 6 98 PS50209 Caspase Recruitment
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_434700 536 212050,607212 99.8 100.0 NP_434701 492 212050,607212 99.0 90.9 XP_011513889 1153 606445,607210,615206,616452 39.9 99.1 NP_001311210 1154 606445,607210,615206,616452 39.9 99.1 XP_011513888 1154 606445,607210,615206,616452 39.9 99.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9953 Download

>KIBB9953  1608 bp
ATGTCGGACTACGAGAACGATGACGAGTGCTGGAACGTCCTGGAGGGCTTCCGGGTGACG
CTCACCTCGGTCATCGACCCCTCACGCATCACACCTTACCTGCGGCAGTGCAAGGTCCTG
AACCCTGATGATGAGGAGCAGGTGCTCAGCGACCCCAACCTGGTCATCCGCAAACGGAAA
GTGGGTGTGCTCCTGGACATCCTGCAGCGGACCGGCCACAAGGGCTACGTGGCCTTCCTC
GAGAGCCTGGAGCTCTACTACCCGCAGCTGTACAAGAAGGTCACAGGCAAGGAGCCGGCC
CGCGTCTTCTCCATGATCATCGACGCGTCCGGGGAGTCAGGCCTGACTCAGCTGCTGATG
ACTGAGGTCATGAAGCTGCAGAAGAAGGTGCAGGACCTGACCGCGCTGCTGAGCTCCAAA
GATGACTTCATCAAGGAGCTGCGGGTGAAGGACAGCCTGCTGCGCAAGCACCAGGAGCGT
GTGCAGAGGCTCAAGGAGGAGTGCGAGGCCGGCAGCCGCGAGCTCAAGCGCTGCAAGGAG
GAGAACTACGACCTGGCCATGCGCCTGGCGCACCAGAGTGAGGAGAAGGGCGCCGCGCTC
ATGCGGAACCGTGACCTGCAGCTGGAGATTGACCAGCTCAAGCACAGCCTCATGAAGGCC
GAGGACGACTGCAAGGTGGAGCGCAAGCACACGCTGAAGCTCAGGCACGCCATGGAGCAG
CGGCCCAGCCAGGAGCTGCTGTGGGAGCTGCAGCAGGAGAAGGCCCTGCTCCAGGCCCGG
GTGCAGGAGCTGGAGGCCTCCGTCCAGGAGGGGAAGCTGGACAGGAGCAGCCCCTACATC
CAGGTACTGGAGGAGGACTGGCGGCAGGCGCTGCGGGACCACCAGGAGCAGGCCAACACC
ATCTTCTCCCTGCGCAAGGACCTCCGCCAGGGCGAGGCCCGACGCCTCCGGTGCATGGAG
GAGAAGGAGATGTTCGAGCTGCAGTGCCTGGCACTACGTAAGGACTCCAAGATGTACAAG
GACCGCATCGAGGCCATCCTGCTGCAGATGGAGGAGGTCGCCATTGAGCGGGACCAGGCC
ATAGCCACGCGGGAGGAGCTGCACGCACAGCACGCCCGGGGCCTGCAGGAGAAGGACGCG
CTGCGCAAGCAGGTGCGGGAGCTGGGCGAGAAGGCGGATGAGCTGCAGCTGCAGGTGTTC
CAGTGTGAGGCGCAGCTACTGGCCGTGGAGGGCAGGCTCAGGCGGCAGCAGCTGGAGACG
CTCGTCCTGAGCTCCGACCTGGAAGATGGCTCACCCAGGAGGTCCCAGGAGCTCTCACTC
CCCCAGGACCTGGAGGACACCCAGCTCTCAGACAAAGGCTGCCTTGCCGGCGGGGGGAGC
CCGAAACAGCCCTTTGCAGCTCTGCACCAGGAGCAGGTTTTGCGGAACCCCCATGACGCA
GGCCTGAGCAGCGGGGAGCCGCCCGAGAAGGAGCGGCGGCGCCTCAAAGAGAGTTTTGAG
AACTACCGCAGGAAGCGCGCCCTCAGGAAGATGCAGAAAGGATGGCGGCAGGGGGAGGAG
GACCGGGAGAACACCACGGGCAGCGACAACACCGACACTGAGGGCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AB9953 Download

>KIBB9953  536 aa
MSDYENDDECWNVLEGFRVTLTSVIDPSRITPYLRQCKVLNPDDEEQVLSDPNLVIRKRK
VGVLLDILQRTGHKGYVAFLESLELYYPQLYKKVTGKEPARVFSMIIDASGESGLTQLLM
TEVMKLQKKVQDLTALLSSKDDFIKELRVKDSLLRKHQERVQRLKEECEAGSRELKRCKE
ENYDLAMRLAHQSEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQ
RPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANT
IFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQA
IATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLET
LVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDA
GLSSGEPPEKERRRLKESFENYRRKRALRKMQKGWRQGEEDRENTTGSDNTDTEGS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9953 Download

>pFN21AB9953  6313 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGACTACGAGAACGATGACGAGTGCTGGAA
CGTCCTGGAGGGCTTCCGGGTGACGCTCACCTCGGTCATCGACCCCTCACGCATCACACC
TTACCTGCGGCAGTGCAAGGTCCTGAACCCTGATGATGAGGAGCAGGTGCTCAGCGACCC
CAACCTGGTCATCCGCAAACGGAAAGTGGGTGTGCTCCTGGACATCCTGCAGCGGACCGG
CCACAAGGGCTACGTGGCCTTCCTCGAGAGCCTGGAGCTCTACTACCCGCAGCTGTACAA
GAAGGTCACAGGCAAGGAGCCGGCCCGCGTCTTCTCCATGATCATCGACGCGTCCGGGGA
GTCAGGCCTGACTCAGCTGCTGATGACTGAGGTCATGAAGCTGCAGAAGAAGGTGCAGGA
CCTGACCGCGCTGCTGAGCTCCAAAGATGACTTCATCAAGGAGCTGCGGGTGAAGGACAG
CCTGCTGCGCAAGCACCAGGAGCGTGTGCAGAGGCTCAAGGAGGAGTGCGAGGCCGGCAG
CCGCGAGCTCAAGCGCTGCAAGGAGGAGAACTACGACCTGGCCATGCGCCTGGCGCACCA
GAGTGAGGAGAAGGGCGCCGCGCTCATGCGGAACCGTGACCTGCAGCTGGAGATTGACCA
GCTCAAGCACAGCCTCATGAAGGCCGAGGACGACTGCAAGGTGGAGCGCAAGCACACGCT
GAAGCTCAGGCACGCCATGGAGCAGCGGCCCAGCCAGGAGCTGCTGTGGGAGCTGCAGCA
GGAGAAGGCCCTGCTCCAGGCCCGGGTGCAGGAGCTGGAGGCCTCCGTCCAGGAGGGGAA
GCTGGACAGGAGCAGCCCCTACATCCAGGTACTGGAGGAGGACTGGCGGCAGGCGCTGCG
GGACCACCAGGAGCAGGCCAACACCATCTTCTCCCTGCGCAAGGACCTCCGCCAGGGCGA
GGCCCGACGCCTCCGGTGCATGGAGGAGAAGGAGATGTTCGAGCTGCAGTGCCTGGCACT
ACGTAAGGACTCCAAGATGTACAAGGACCGCATCGAGGCCATCCTGCTGCAGATGGAGGA
GGTCGCCATTGAGCGGGACCAGGCCATAGCCACGCGGGAGGAGCTGCACGCACAGCACGC
CCGGGGCCTGCAGGAGAAGGACGCGCTGCGCAAGCAGGTGCGGGAGCTGGGCGAGAAGGC
GGATGAGCTGCAGCTGCAGGTGTTCCAGTGTGAGGCGCAGCTACTGGCCGTGGAGGGCAG
GCTCAGGCGGCAGCAGCTGGAGACGCTCGTCCTGAGCTCCGACCTGGAAGATGGCTCACC
CAGGAGGTCCCAGGAGCTCTCACTCCCCCAGGACCTGGAGGACACCCAGCTCTCAGACAA
AGGCTGCCTTGCCGGCGGGGGGAGCCCGAAACAGCCCTTTGCAGCTCTGCACCAGGAGCA
GGTTTTGCGGAACCCCCATGACGCAGGCCTGAGCAGCGGGGAGCCGCCCGAGAAGGAGCG
GCGGCGCCTCAAAGAGAGTTTTGAGAACTACCGCAGGAAGCGCGCCCTCAGGAAGATGCA
GAAAGGATGGCGGCAGGGGGAGGAGGACCGGGAGAACACCACGGGCAGCGACAACACCGA
CACTGAGGGCTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT