Restriction map for
pFN21AB9953
(Linear) (Six-base) MAPSORT of: KIBB9953.seq Check: 9567 from: 1 to: 1608
KIBB9953.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 1140 1182 1608
Size: 129 1011 42 426
Fragments arranged by size:
1011 426 129 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1608
Size: 715 893
AfeI AGC'GCT
Cuts at: 0 529 1608
Size: 529 1079
AflIII A'CryG_T
Cuts at: 0 323 1608
Size: 323 1285
AleI CACnn'nnGTG
Cuts at: 0 575 1608
Size: 575 1033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1168 1608
Size: 1168 440
AlwNI CAG_nnn'CTG
Cuts at: 0 354 396 735 789 846 870 987 1218
Size: 354 42 339 54 57 24 117 231
Cuts at: 1218 1251 1329 1608
Size: 33 78 279
Fragments arranged by size:
354 339 279 231 117 78 57 54
42 33 24
AvaI C'yCGr_G
Cuts at: 0 238 776 1115 1462 1608
Size: 238 538 339 347 146
Fragments arranged by size:
538 347 339 238 146
BanI G'GyrC_C
Cuts at: 0 589 1478 1608
Size: 589 889 130
Fragments arranged by size:
889 589 130
BanII G_rGCy'C
Cuts at: 0 254 415 524 1273 1313 1381 1606 1608
Size: 254 161 109 749 40 68 225 2
Fragments arranged by size:
749 254 225 161 109 68 40 2
BbeI G_GCGC'C
Cuts at: 0 593 1482 1608
Size: 593 889 126
Fragments arranged by size:
889 593 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 1608
Size: 297 1311
BclI T'GATC_A
Cuts at: 0 314 1608
Size: 314 1294
BglI GCCn_nnn'nGGC
Cuts at: 0 221 1367 1608
Size: 221 1146 241
Fragments arranged by size:
1146 241 221
BlpI GC'TnA_GC
Cuts at: 0 145 409 1608
Size: 145 264 1199
Fragments arranged by size:
1199 264 145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 268 643 754 811 868 1273 1351
Size: 61 207 375 111 57 57 405 78
Cuts at: 1351 1608
Size: 257
Fragments arranged by size:
405 375 257 207 111 78 61 57
57
Bpu10I CC'TnA_GC
Cuts at: 0 700 1237 1267 1444 1608
Size: 700 537 30 177 164
Fragments arranged by size:
700 537 177 164 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 508 622 1608
Size: 475 33 114 986
Fragments arranged by size:
986 475 114 33
BsaAI yAC'GTr
Cuts at: 0 228 997 1608
Size: 228 769 611
Fragments arranged by size:
769 611 228
BsaHI Gr'CG_yC
Cuts at: 0 590 942 1479 1608
Size: 590 352 537 129
Fragments arranged by size:
590 537 352 129
BsaWI w'CCGG_w
Cuts at: 0 947 1608
Size: 947 661
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 724 754 1608
Size: 724 30 854
Fragments arranged by size:
854 724 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 511 553 595 865 973 1066 1108
Size: 148 363 42 42 270 108 93 42
Cuts at: 1108 1570 1608
Size: 462 38
Fragments arranged by size:
462 363 270 148 108 93 42 42
42 38
BseYI C'CCAG_C
Cuts at: 0 724 1161 1340 1608
Size: 724 437 179 268
Fragments arranged by size:
724 437 268 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 502 802 1084 1387 1608
Size: 409 93 300 282 303 221
Fragments arranged by size:
409 303 300 282 221 93
BsiHKAI G_wGCw'C
Cuts at: 0 146 191 254 415 524 1273 1313 1608
Size: 146 45 63 161 109 749 40 295
Fragments arranged by size:
749 295 161 146 109 63 45 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1249 1608
Size: 1249 359
Bsp1286I G_dGCh'C
Cuts at: 0 146 191 254 415 524 1273 1313 1381
Size: 146 45 63 161 109 749 40 68
Cuts at: 1381 1606 1608
Size: 225 2
Fragments arranged by size:
749 225 161 146 109 68 63 45
40 2
BspHI T'CATG_A
Cuts at: 0 368 650 1608
Size: 368 282 958
Fragments arranged by size:
958 368 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 129 623 1140 1182 1404 1608
Size: 107 22 494 517 42 222 204
Fragments arranged by size:
517 494 222 204 107 42 22
BsrBI CCG'CTC
Cuts at: 0 1068 1473 1608
Size: 1068 405 135
Fragments arranged by size:
1068 405 135
BsrFI r'CCGG_y
Cuts at: 0 211 294 508 1366 1608
Size: 211 83 214 858 242
Fragments arranged by size:
858 242 214 211 83
BsrGI T'GTAC_A
Cuts at: 0 269 1013 1608
Size: 269 744 595
Fragments arranged by size:
744 595 269
BssHII G'CGCG_C
Cuts at: 0 1515 1608
Size: 1515 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 870 1419 1608
Size: 870 549 189
Fragments arranged by size:
870 549 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 564 1608
Size: 564 1044
Bsu36I CC'TnA_GG
Cuts at: 0 1522 1608
Size: 1522 86
BtgI C'CryG_G
Cuts at: 0 710 1223 1574 1608
Size: 710 513 351 34
Fragments arranged by size:
710 513 351 34
BtsI GCAGTG_nn'
Cuts at: 0 112 988 1608
Size: 112 876 620
Fragments arranged by size:
876 620 112
EaeI y'GGCC_r
Cuts at: 0 213 554 1220 1608
Size: 213 341 666 388
Fragments arranged by size:
666 388 341 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 913 1189 1608
Size: 913 276 419
Fragments arranged by size:
913 419 276
EcoICRI GAG'CTC
Cuts at: 0 252 413 522 1271 1311 1608
Size: 252 161 109 749 40 297
Fragments arranged by size:
749 297 252 161 109 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 268 643 715 754 811 868 1273
Size: 61 207 375 72 39 57 57 405
Cuts at: 1273 1351 1608
Size: 78 257
Fragments arranged by size:
405 375 257 207 78 72 61 57
57 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 769 913 925 1608
Size: 652 117 144 12 683
Fragments arranged by size:
683 652 144 117 12
EcoO109I rG'GnC_Cy
Cuts at: 0 114 393 762 918 1119 1301 1326 1608
Size: 114 279 369 156 201 182 25 282
Fragments arranged by size:
369 282 279 201 182 156 114 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1378 1608
Size: 1378 230
FspI TGC'GCA
Cuts at: 0 463 913 1144 1608
Size: 463 450 231 464
Fragments arranged by size:
464 463 450 231
HaeII r_GCGC'y
Cuts at: 0 531 593 871 1482 1608
Size: 531 62 278 611 126
Fragments arranged by size:
611 531 278 126 62
KasI G'GCGC_C
Cuts at: 0 589 1478 1608
Size: 589 889 130
Fragments arranged by size:
889 589 130
MluI A'CGCG_T
Cuts at: 0 323 1608
Size: 323 1285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 1608
Size: 1298 310
MscI TGG'CCA
Cuts at: 0 556 1608
Size: 556 1052
MslI CAynn'nnrTG
Cuts at: 0 575 1608
Size: 575 1033
MspA1I CmG'CkG
Cuts at: 0 207 267 351 621 720 1188 1251 1451
Size: 207 60 84 270 99 468 63 200
Cuts at: 1451 1608
Size: 157
Fragments arranged by size:
468 270 207 200 157 99 84 63
60
NaeI GCC'GGC
Cuts at: 0 296 510 1368 1608
Size: 296 214 858 240
Fragments arranged by size:
858 296 240 214
NarI GG'CG_CC
Cuts at: 0 590 1479 1608
Size: 590 889 129
Fragments arranged by size:
889 590 129
NcoI C'CATG_G
Cuts at: 0 710 1608
Size: 710 898
NgoMIV G'CCGG_C
Cuts at: 0 294 508 1366 1608
Size: 294 214 858 242
Fragments arranged by size:
858 294 242 214
PflMI CCAn_nnn'nTGG
Cuts at: 0 846 1329 1608
Size: 846 483 279
Fragments arranged by size:
846 483 279
PfoI T'CCnGG_A
Cuts at: 0 38 191 803 1608
Size: 38 153 612 805
Fragments arranged by size:
805 612 153 38
PpuMI rG'GwC_Cy
Cuts at: 0 114 393 918 1301 1326 1608
Size: 114 279 525 383 25 282
Fragments arranged by size:
525 383 282 279 114 25
PshAI GACnn'nnGTC
Cuts at: 0 364 1261 1608
Size: 364 897 347
Fragments arranged by size:
897 364 347
PstI C_TGCA'G
Cuts at: 0 206 380 620 752 983 1046 1127 1187
Size: 206 174 240 132 231 63 81 60
Cuts at: 1187 1193 1608
Size: 6 415
Fragments arranged by size:
415 240 231 206 174 132 81 63
60 6
PvuII CAG'CTG
Cuts at: 0 267 351 621 1188 1251 1608
Size: 267 84 270 567 63 357
Fragments arranged by size:
567 357 270 267 84 63
RsrII CG'GwC_CG
Cuts at: 0 209 1608
Size: 209 1399
SacI G_AGCT'C
Cuts at: 0 254 415 524 1273 1313 1608
Size: 254 161 109 749 40 295
Fragments arranged by size:
749 295 254 161 109 40
SbfI CC_TGCA'GG
Cuts at: 0 1127 1608
Size: 1127 481
SexAI A'CCwGG_T
Cuts at: 0 158 1608
Size: 158 1450
SfcI C'TryA_G
Cuts at: 0 202 376 616 748 979 1042 1123 1183
Size: 202 174 240 132 231 63 81 60
Cuts at: 1183 1189 1608
Size: 6 419
Fragments arranged by size:
419 240 231 202 174 132 81 63
60 6
SfoI GGC'GCC
Cuts at: 0 591 1480 1608
Size: 591 889 128
Fragments arranged by size:
889 591 128
SmaI CCC'GGG
Cuts at: 0 778 1117 1608
Size: 778 339 491
Fragments arranged by size:
778 491 339
SmlI C'TyrA_G
Cuts at: 0 238 490 523 637 1608
Size: 238 252 33 114 971
Fragments arranged by size:
971 252 238 114 33
SnaBI TAC'GTA
Cuts at: 0 997 1608
Size: 997 611
StuI AGG'CCT
Cuts at: 0 341 796 1442 1608
Size: 341 455 646 166
Fragments arranged by size:
646 455 341 166
StyI C'CwwG_G
Cuts at: 0 710 1608
Size: 710 898
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 172 1308 1354 1608
Size: 57 115 1136 46 254
Fragments arranged by size:
1136 254 115 57 46
TatI w'GTAC_w
Cuts at: 0 269 1013 1608
Size: 269 744 595
Fragments arranged by size:
744 595 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 168 200 724 756 1608
Size: 168 32 524 32 852
Fragments arranged by size:
852 524 168 32 32
XhoI C'TCGA_G
Cuts at: 0 238 1608
Size: 238 1370
XmaI C'CCGG_G
Cuts at: 0 776 1115 1608
Size: 776 339 493
Fragments arranged by size:
776 493 339
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI AvaI
BanI BanII BbeI BbsI BclI BglI BlpI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstXI Bsu36I BtgI BtsI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PflMI PfoI PpuMI PshAI PstI PvuII
RsrII SacI SbfI SexAI SfcI SfoI SmaI SmlI
SnaBI StuI StyI TaqII TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglII Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaBI BsiEI BsiWI BsmI
BspEI BsrDI BssSI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI
EcoRV FseI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI SacII SalI SanDI SapI ScaI SfiI
SgfI SgrAI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XcmI XmnI ZraI