Restriction map for
pFN21AB9953
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BsaBI BsaI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BsrDI BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I ClaI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SapI
ScaI SfiI SfuI SgrAI SpeI
SphI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoII
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 323 ACGCGT A^C[GA][CT]GT
AocI 1521 CCTCAGG CC^T[ACGT]AGG
Aor51HI 527 AGCGCT AGC^GCT
BalI 554 TGGCCA TGG^CCA
BbsI 304 GTCTTC GAAGAC(2/6)
BclI 314 TGATCA T^GATCA
BpuAI 304 GTCTTC GAAGAC(2/6)
BsaWI 947 TCCGGT [AT]^CCGG[AT]
BsmAI 1255 GAGAC GTCTC(1/5)
BsmBI 1255 GAGACG CGTCTC(1/5)
BssHII 1515 GCGCGC G^CGCGC
BstXI 557 CCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1521 CCTCAGG CC^T[ACGT]AGG
CpoI 208 CGGACCG CG^G[AT]CCG
DpnII 315 GATC ^GATC
Eco47III 527 AGCGCT AGC^GCT
Eco81I 1521 CCTCAGG CC^T[ACGT]AGG
EcoT14I 710 CCATGG C^C[AT][AT]GG
FbaI 314 TGATCA T^GATCA
MboI 315 GATC ^GATC
MluI 323 ACGCGT A^CGCGT
MluNI 554 TGGCCA TGG^CCA
MscI 554 TGGCCA TGG^CCA
MslI 571 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 710 CCATGG C^CATGG
NdeII 315 GATC ^GATC
PaeR7I 238 CTCGAG C^TCGAG
RsrII 208 CGGACCG CG^G[AT]CCG
Sau3AI 315 GATC ^GATC
SbfI 1122 CCTGCAGG CCTGCA^GG
SexAI 158 ACCTGGT A^CC[AT]GGT
SnaBI 995 TACGTA TAC^GTA
Sse8387I 1122 CCTGCAGG CCTGCA^GG
StyI 710 CCATGG C^C[AT][AT]GG
XhoI 238 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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BanI 589 GGCGCC G^G[CT][GA]CC
1478 GGCGCC
BbeI 589 GGCGCC GGCGC^C
1478 GGCGCC
BglI 215 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1361 GCCTTGCCGGC
BlpI 144 GCTCAGC GC^T[ACGT]AGC
408 GCTGAGC
Bpu1102I 144 GCTCAGC GC^T[ACGT]AGC
408 GCTGAGC
BsaAI 226 TACGTG [CT]AC^GT[GA]
995 TACGTA
Bsp1407I 269 TGTACA T^GTACA
1013 TGTACA
BspHI 368 TCATGA T^CATGA
650 TCATGA
BsrBI 1066 GAGCGG CCGCTC(-3/-3)
1471 GAGCGG
BsrGI 269 TGTACA T^GTACA
1013 TGTACA
BstAPI 864 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1413 GCAGGTTTTGC
CelII 144 GCTCAGC GC^T[ACGT]AGC
408 GCTGAGC
KasI 589 GGCGCC G^GCGCC
1478 GGCGCC
NarI 589 GGCGCC GG^CGCC
1478 GGCGCC
PflMI 840 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1323 CCAGGACCTGG
PshAI 360 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1257 GACGCTCGTC
RcaI 368 TCATGA T^CATGA
650 TCATGA
SfoI 589 GGCGCC GGC^GCC
1478 GGCGCC
SmaI 776 CCCGGG CCC^GGG
1115 CCCGGG
SspBI 269 TGTACA T^GTACA
1013 TGTACA
Van91I 840 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1323 CCAGGACCTGG
XmaCI 776 CCCGGG C^CCGGG
1115 CCCGGG
XmaI 776 CCCGGG C^CCGGG
1115 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII AviII
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau96I ScrFI
SfaNI SfcI SmlI StuI TaqI
TseI Tsp45I TspRI TthHB8I