PORCN ORF clone pFN21AB5192

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5192

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Clone info.

Species

human

Clone Name

pFN21AB5192 Sequence (with vector) Restriction Map

Product ID

FHC11582

Flexi Clone PID

FXC11582

ORF Sequence

DNA sequence (1383 bp)
Protein sequence (461 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529146]

Gene

KIBB5192

Symbol

PORCN

Alias : DHOF, FODH, MG61, PORC, PPN

Description

porcupine homolog (Drosophila), transcript variant D

Original Clone ID

bm01545

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1383 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 461 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14299.1 0 100.0 PORCN CCDS14297.1 0 98.9 PORCN CCDS14298.1 8.3e-219 98.5 PORCN CCDS14296.1 1.3e-108 97.4 PORCN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004299 117 401 PF03062 Membrane bound O-acyl transferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 16 GCLLPTAQQGLDQIWLLLAICLA 38 SECONDARY 23 2 61 FFSLYHFFQLHMVWVVLLSLLCY 83 PRIMARY 23 3 93 SHRGVFLSVTILIYLLMGEMHMV 115 SECONDARY 23 4 153 VEFMGYLYFVGTIVFGPWISFHS 175 SECONDARY 23 5 193 VARSLALALLCLVLSTCVGPYLF 215 SECONDARY 23 6 332 VTYAASALLHGFSFHLAAVLLSL 354 PRIMARY 23 7 396 RALNLLFGALAIFHLAYLGSLFD 418 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_006724609 461 300651,305600 100.0 100.0 NP_982301 461 300651,305600 100.0 100.0 XP_005272694 545 300651,305600 100.0 100.0 XP_016885224 574 300651,305600 100.0 100.0 NP_982299 456 300651,305600 98.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5192 Download

>KIBB5192  1383 bp
ATGGCCACCTTTAGCCGCCAGGAATTTTTCCAGCAGCTACTGCAAGGCTGTCTCCTGCCT
ACTGCCCAGCAGGGCCTTGACCAGATCTGGCTGCTCCTTGCCATCTGCCTCGCCTGCCGC
CTCCTCTGGAGGCTCGGGTTGCCATCCTACCTGAAGCATGCAAGCACCGTGGCAGGCGGG
TTCTTCAGCCTCTACCACTTCTTCCAGCTGCACATGGTTTGGGTCGTGCTGCTCAGCCTC
CTGTGCTACCTCGTGCTGTTCCTCTGCCGACATTCCTCCCATCGAGGCGTCTTCCTATCC
GTCACCATCCTCATCTACCTACTCATGGGTGAGATGCACATGGTAGACACCGTGACATGG
CACAAGATGCGAGGGGCACAGATGATTGTGGCCATGAAGGCAGTGTCTCTGGGCTTCGAC
CTGGACCGGGGCGAGGTGGGTACGGTGCCCTCGCCAGTGGAGTTCATGGGCTACCTCTAC
TTCGTGGGCACCATCGTCTTCGGGCCCTGGATATCCTTCCACAGCTACCTACAAGCTGTC
CAAGGCCGCCCACTGAGCTGCCGGTGGCTGCAGAAGGTGGCCCGGAGCCTGGCACTGGCC
CTGCTGTGCCTTGTGCTGTCCACTTGCGTGGGCCCCTACCTCTTCCCGTACTTCATCCCC
CTCAACGGTGACCGCCTCCTTCGCAACAAGAAACGCAAAGCCAGGGGCACCATGGTAAGG
TGGCTGCGAGCCTACGAGAGTGCTGTCTCCTTCCACTTCAGCAACTATTTTGTGGGCTTT
CTTTCCGAGGCCACGGCCACGTTGGCGGGGGCTGGCTTTACCGAGGAGAAGGATCACCTG
GAATGGGACCTGACGGTGTCCAAGCCACTGAATGTGGAGCTGCCTCGGTCAATGGTGGAA
GTTGTCACAAGCTGGAACCTGCCCATGTCTTATTGGCTAAATAACTATGTTTTCAAGAAT
GCTCTCCGCCTGGGGACCTTCTCGGCTGTGCTGGTCACCTATGCAGCCAGCGCCCTCCTA
CATGGCTTCAGTTTCCACCTGGCTGCGGTCCTGCTGTCCCTGGCTTTTATCACTTACGTG
GAGCATGTCCTCCGGAAGCGCCTGGCTCGGATCCTCAGTGCCTGTGTCTTGTCAAAGCGG
TGCCCGCCAGACTGTTCGCACCAGCATCGCTTGGGCCTGGGGGTGCGAGCCTTAAACTTG
CTCTTTGGAGCTCTGGCCATCTTCCACCTGGCCTACCTGGGCTCCCTGTTTGATGTCGAT
GTGGATGACACCACAGAGGAGCAGGGCTACGGCATGGCATACACTGTCCACAAGTGGTCA
GAGCTCAGCTGGGCCAGTCACTGGGTCACTTTTGGATGCTGGATCTTCTACCGTCTCATA
GGC

ORF Protein

Cloned ORF protein sequence for pFN21AB5192 Download

>KIBB5192  461 aa
MATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGG
FFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTW
HKMRGAQMIVAMKAVSLGFDLDRGEVGTVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAV
QGRPLSCRWLQKVARSLALALLCLVLSTCVGPYLFPYFIPLNGDRLLRNKKRKARGTMVR
WLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSKPLNVELPRSMVE
VVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYV
EHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVD
VDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5192 Download

>pFN21AB5192  6088 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCACCTTTAGCCGCCAGGAATTTTTCCAGCA
GCTACTGCAAGGCTGTCTCCTGCCTACTGCCCAGCAGGGCCTTGACCAGATCTGGCTGCT
CCTTGCCATCTGCCTCGCCTGCCGCCTCCTCTGGAGGCTCGGGTTGCCATCCTACCTGAA
GCATGCAAGCACCGTGGCAGGCGGGTTCTTCAGCCTCTACCACTTCTTCCAGCTGCACAT
GGTTTGGGTCGTGCTGCTCAGCCTCCTGTGCTACCTCGTGCTGTTCCTCTGCCGACATTC
CTCCCATCGAGGCGTCTTCCTATCCGTCACCATCCTCATCTACCTACTCATGGGTGAGAT
GCACATGGTAGACACCGTGACATGGCACAAGATGCGAGGGGCACAGATGATTGTGGCCAT
GAAGGCAGTGTCTCTGGGCTTCGACCTGGACCGGGGCGAGGTGGGTACGGTGCCCTCGCC
AGTGGAGTTCATGGGCTACCTCTACTTCGTGGGCACCATCGTCTTCGGGCCCTGGATATC
CTTCCACAGCTACCTACAAGCTGTCCAAGGCCGCCCACTGAGCTGCCGGTGGCTGCAGAA
GGTGGCCCGGAGCCTGGCACTGGCCCTGCTGTGCCTTGTGCTGTCCACTTGCGTGGGCCC
CTACCTCTTCCCGTACTTCATCCCCCTCAACGGTGACCGCCTCCTTCGCAACAAGAAACG
CAAAGCCAGGGGCACCATGGTAAGGTGGCTGCGAGCCTACGAGAGTGCTGTCTCCTTCCA
CTTCAGCAACTATTTTGTGGGCTTTCTTTCCGAGGCCACGGCCACGTTGGCGGGGGCTGG
CTTTACCGAGGAGAAGGATCACCTGGAATGGGACCTGACGGTGTCCAAGCCACTGAATGT
GGAGCTGCCTCGGTCAATGGTGGAAGTTGTCACAAGCTGGAACCTGCCCATGTCTTATTG
GCTAAATAACTATGTTTTCAAGAATGCTCTCCGCCTGGGGACCTTCTCGGCTGTGCTGGT
CACCTATGCAGCCAGCGCCCTCCTACATGGCTTCAGTTTCCACCTGGCTGCGGTCCTGCT
GTCCCTGGCTTTTATCACTTACGTGGAGCATGTCCTCCGGAAGCGCCTGGCTCGGATCCT
CAGTGCCTGTGTCTTGTCAAAGCGGTGCCCGCCAGACTGTTCGCACCAGCATCGCTTGGG
CCTGGGGGTGCGAGCCTTAAACTTGCTCTTTGGAGCTCTGGCCATCTTCCACCTGGCCTA
CCTGGGCTCCCTGTTTGATGTCGATGTGGATGACACCACAGAGGAGCAGGGCTACGGCAT
GGCATACACTGTCCACAAGTGGTCAGAGCTCAGCTGGGCCAGTCACTGGGTCACTTTTGG
ATGCTGGATCTTCTACCGTCTCATAGGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT