(Circular) (Six-base) MAPSORT of: pFN21AB5192.seq Check: 5129 from: 1 to: 6088 pFN21AB5192 6088 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2349 3431 1937 Size: 412 1082 4594 Fragments arranged by size: 4594 1082 412 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 2177 2746 3016 4151 5535 5803 1632 Size: 541 4 569 270 1135 1384 268 1917 Fragments arranged by size: 1917 1384 1135 569 541 270 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 2630 3080 1166 Size: 1464 450 4174 Fragments arranged by size: 4174 1464 450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2044 3126 3345 3831 5403 2044 Size: 1082 219 486 1572 2729 Fragments arranged by size: 2729 1572 1082 486 219 ApaI G_GGCC'C Cuts at: 2511 2639 2511 Size: 128 5960 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2027 3397 3623 3775 5884 1493 Size: 534 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 534 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2138 3413 1870 Size: 73 195 1275 4545 Fragments arranged by size: 4545 1275 195 73 BamHI G'GATC_C Cuts at: 3114 3418 3785 3114 Size: 304 367 5417 Fragments arranged by size: 5417 367 304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 2492 2711 3144 3409 Size: 325 217 627 662 43 219 433 265 Cuts at: 3409 4864 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 662 627 433 325 265 219 217 43 BanII G_rGCy'C Cuts at: 1391 2511 2639 3217 3248 3330 3407 1391 Size: 1120 128 578 31 82 77 4072 Fragments arranged by size: 4072 1120 578 128 82 77 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2287 2494 928 Size: 971 388 207 4522 Fragments arranged by size: 4522 971 388 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3152 3186 4279 4313 1903 Size: 34 1215 34 1093 34 3678 Fragments arranged by size: 3678 1215 1093 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2402 2807 4699 Size: 107 122 71 631 605 730 405 1892 Cuts at: 4699 136 Size: 1525 Fragments arranged by size: 1892 1525 730 631 605 405 122 107 71 BglII A'GATC_T Cuts at: 2088 6083 2088 Size: 3995 2093 BlpI GC'TnA_GC Cuts at: 2237 3329 3505 2237 Size: 1092 176 4820 Fragments arranged by size: 4820 1092 176 Bme1580I G_kGCm'C Cuts at: 1790 2383 2454 2495 2511 2639 2714 3149 4149 Size: 593 71 41 16 128 75 435 1000 Cuts at: 4149 5305 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1000 593 435 128 75 71 41 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3355 3819 4777 Size: 416 53 83 399 24 1938 464 958 Cuts at: 4777 442 Size: 1753 Fragments arranged by size: 1938 1753 958 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2152 4748 1239 Size: 913 2596 2579 Fragments arranged by size: 2596 2579 913 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3082 3879 493 Size: 711 1878 797 2702 Fragments arranged by size: 2702 1878 797 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 3895 4277 4912 Size: 53 83 186 936 759 1603 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1603 1451 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3096 4521 4919 5193 5340 5751 3096 Size: 1425 398 274 147 411 3433 Fragments arranged by size: 3433 1425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3230 3260 1178 Size: 30 165 30 1827 30 4006 Fragments arranged by size: 4006 1827 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2843 3296 1186 Size: 931 726 453 3978 Fragments arranged by size: 3978 931 726 453 BseYI C'CCAG_C Cuts at: 2070 3333 5291 5787 2070 Size: 1263 1958 496 2371 Fragments arranged by size: 2371 1958 1263 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 5053 Fragments arranged by size: 5053 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3217 3330 4149 4234 5305 3217 Size: 113 819 85 1071 4000 Fragments arranged by size: 4000 1071 819 113 85 BsmI GAATG_Cn' Cuts at: 2968 3599 3692 2968 Size: 631 93 5364 Fragments arranged by size: 5364 631 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3383 3383 Size: 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2383 2454 2495 2511 2639 2714 3149 Size: 399 593 71 41 16 128 75 435 Cuts at: 3149 3217 3248 3330 3407 4149 4234 5305 1391 Size: 68 31 82 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 593 435 399 128 85 82 77 75 71 68 41 31 16 BspEI T'CCGG_A Cuts at: 3096 5751 3096 Size: 2655 3433 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2931 3443 844 Size: 2087 512 3489 Fragments arranged by size: 3489 2087 512 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2565 4732 4919 1700 Size: 110 755 2167 187 2869 Fragments arranged by size: 2869 2167 755 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2255 4148 5160 2255 Size: 1893 1012 3183 Fragments arranged by size: 3183 1893 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2044 2371 794 Size: 1250 327 4511 Fragments arranged by size: 4511 1250 327 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 2672 2998 1184 Size: 1488 326 4274 Fragments arranged by size: 4274 1488 326 BstXI CCAn_nnnn'nTGG Cuts at: 2632 2632 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2088 3114 3366 3418 3785 4170 4187 5639 Size: 116 1026 252 52 367 385 17 1452 Cuts at: 5639 5725 5737 6083 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 252 116 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2715 2796 513 Size: 552 939 168 543 81 3805 Fragments arranged by size: 3805 939 552 543 168 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3155 487 Size: 683 56 1929 3420 Fragments arranged by size: 3420 1929 683 56 BtsI GCAGTG_nn' Cuts at: 2412 3604 4398 4418 2412 Size: 1192 794 20 4082 Fragments arranged by size: 4082 1192 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2154 2189 529 Size: 35 1590 35 4428 Fragments arranged by size: 4428 1590 35 35 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2394 2799 Size: 54 1425 50 275 18 177 387 405 Cuts at: 2799 3219 3535 4424 6053 8 Size: 420 316 889 1629 43 Fragments arranged by size: 1629 1425 889 420 405 387 316 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 4018 1353 Size: 1298 1367 3423 Fragments arranged by size: 3423 1367 1298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2960 4675 5049 5195 387 Size: 1064 138 1371 1715 374 146 1280 Fragments arranged by size: 1715 1371 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3215 3328 3215 Size: 113 5975 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2173 2177 2746 3016 4151 4748 Size: 393 520 21 4 569 270 1135 597 Cuts at: 4748 5535 5803 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1135 787 597 569 520 393 270 268 21 4 EcoO109I rG'GnC_Cy Cuts at: 2077 2508 2636 2851 2979 2077 Size: 431 128 215 128 5186 Fragments arranged by size: 5186 431 215 128 128 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 EcoRV GAT'ATC Cuts at: 2517 2517 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 3018 3106 5235 1678 Size: 1340 88 2129 2531 Fragments arranged by size: 2531 2129 1340 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3351 3383 4729 4761 4803 Size: 32 135 32 1779 32 1346 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 1779 1346 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2396 3221 10 Size: 54 1425 343 177 387 825 2877 Fragments arranged by size: 2877 1425 825 387 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3080 4046 4405 4564 518 Size: 648 1464 450 966 359 159 2042 Fragments arranged by size: 2042 1464 966 648 450 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 3333 3504 4181 5329 5574 1606 Size: 606 1121 171 677 1148 245 2120 Fragments arranged by size: 2120 1148 1121 677 606 245 171 NcoI C'CATG_G Cuts at: 513 1065 2004 2715 513 Size: 552 939 711 3886 Fragments arranged by size: 3886 939 711 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2165 3092 3446 4991 1132 Size: 97 936 927 354 1545 2229 Fragments arranged by size: 2229 1545 936 927 354 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 3345 1108 Size: 2237 3851 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2851 2979 2851 Size: 128 5960 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2507 2635 2507 Size: 128 5960 PstI C_TGCA'G Cuts at: 830 2577 3440 830 Size: 1747 863 3478 Fragments arranged by size: 3478 1747 863 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 2212 3333 2212 Size: 1121 4967 SacI G_AGCT'C Cuts at: 3217 3330 3217 Size: 113 5975 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2573 3436 4571 5252 5443 826 Size: 219 1528 863 1135 681 191 1471 Fragments arranged by size: 1528 1471 1135 863 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4192 5093 5355 820 Size: 197 926 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 2165 3446 1132 Size: 1033 1281 3774 Fragments arranged by size: 3774 1281 1033 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 2545 2715 3527 513 Size: 552 939 541 170 812 3074 Fragments arranged by size: 3074 939 812 552 541 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 2880 4115 4132 4285 4470 4915 Size: 1255 741 559 1235 17 153 185 445 Cuts at: 4915 325 Size: 1498 Fragments arranged by size: 1498 1255 1235 741 559 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3106 3138 6048 6080 3106 Size: 32 2910 32 3114 Fragments arranged by size: 3114 2910 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2860 1316 Size: 1544 4544 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2713 2803 3229 2713 Size: 90 426 5572 Fragments arranged by size: 5572 426 90 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI