Restriction map for
pFN21AB5192
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BlnI BsaBI BsaI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 343 GTAGAC GT^[AC][GT]AC
AccIII 1091 TCCGGA T^CCGGA
AcsI 22 GAATTT [GA]^AATT[CT]
AcyI 286 GGCGTC G[GA]^CG[CT]C
ApoI 22 GAATTT [GA]^AATT[CT]
AspI 852 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 133 CTCGGG C^[CT]CG[GA]G
BamHI 1109 GGATCC G^GATCC
BcgI 1158 GCACCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 83 AGATCT A^GATCT
BpmI 126 CTGGAG CTGGAG(16/14)
BsaAI 1075 TACGTG [CT]AC^GT[GA]
BsaHI 286 GGCGTC G[GA]^CG[CT]C
BsaWI 1091 TCCGGA [AT]^CCGG[AT]
BseAI 1091 TCCGGA T^CCGGA
BsgI 208 CTGCAC GTGCAG(16/14)
BsmBI 1372 CGTCTC CGTCTC(1/5)
BsmI 957 GAATGC GAATGC(1/-1)
BsoBI 133 CTCGGG C^[CT]CG[GA]G
BspEI 1091 TCCGGA T^CCGGA
BspMI 917 ACCTGC ACCTGC(4/8)
BsrFI 560 GCCGGT [GA]^CCGG[CT]
BssSI 250 CTCGTG CACGAG(-5/-1)
BstXI 620 CCACTTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 560 GCCGGT [GA]^CCGG[CT]
EarI 640 CTCTTC CTCTTC(1/4)
EcoRV 510 GATATC GAT^ATC
HgaI 287 GCGTC GACGC(5/10)
Hin1I 286 GGCGTC G[GA]^CG[CT]C
Ksp632I 640 CTCTTC CTCTTC(1/4)
MroI 1091 TCCGGA T^CCGGA
MseI 1192 TTAA T^TAA
NcoI 710 CCATGG C^CATGG
PflFI 852 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1334 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 568 CTGCAG CTGCA^G
SfcI 568 CTGCAG C^T[GA][CT]AG
SphI 156 GCATGC GCATG^C
Tru9I 1192 TTAA T^TAA
Tsp509I 23 AATT ^AATT
Tth111I 852 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1334 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 440 GTAC GT^AC
648 GTAC
ApaI 502 GGGCCC GGGCC^C
630 GGGCCC
AspHI 1208 GAGCTC G[AT]GC[AT]^C
1321 GAGCTC
BbsI 289 GTCTTC GAAGAC(2/6)
496 GTCTTC
BcnI 426 CCGGG CC^[GC]GG
581 CCCGG
BglI 391 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
796 GCCACGTTGGC
BlpI 231 GCTCAGC GC^T[ACGT]AGC
1323 GCTCAGC
Bpu1102I 231 GCTCAGC GC^T[ACGT]AGC
1323 GCTCAGC
BpuAI 289 GTCTTC GAAGAC(2/6)
496 GTCTTC
BsiHKAI 1208 GAGCTC G[AT]GC[AT]^C
1321 GAGCTC
BstAPI 33 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
360 GCACAAGATGC
BstEII 667 GGTGACC G^GT[ACGT]ACC
993 GGTCACC
BstPI 667 GGTGACC G^GT[ACGT]ACC
993 GGTCACC
CelII 231 GCTCAGC GC^T[ACGT]AGC
1323 GCTCAGC
CfoI 1010 GCGC GCG^C
1098 GCGC
EcoO65I 667 GGTGACC G^GT[ACGT]ACC
993 GGTCACC
EcoT14I 540 CCAAGG C^C[AT][AT]GG
710 CCATGG
HaeII 1009 AGCGCC [GA]GCGC^[CT]
1097 AGCGCC
HhaI 1010 GCGC GCG^C
1098 GCGC
HinP1I 1010 GCGC G^CGC
1098 GCGC
MaeII 799 ACGT A^CGT
1076 ACGT
MslI 621 CACTTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1071 CACTTACGTG
MspA1I 205 CAGCTG C[AC]G^C[GT]G
1326 CAGCTG
NciI 426 CCGGG CC^[GC]GG
581 CCCGG
NspI 156 GCATGC [GA]CATG^[CT]
1083 GCATGT
PpuMI 845 GGGACCT [GA]G^G[AT]CC[CT]
973 GGGACCT
PvuII 205 CAGCTG CAG^CTG
1326 CAGCTG
RsaI 440 GTAC GT^AC
648 GTAC
SacI 1208 GAGCTC GAGCT^C
1321 GAGCTC
StyI 540 CCAAGG C^C[AT][AT]GG
710 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BanI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII