Restriction map for
pFN21AB5192
(Linear) (Six-base) MAPSORT of: KIBB5192.seq Check: 2457 from: 1 to: 1383
KIBB5192.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 344 1383
Size: 344 1039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 172 741 1011 1383
Size: 168 4 569 270 372
Fragments arranged by size:
569 372 270 168 4
AleI CACnn'nnGTG
Cuts at: 0 625 1075 1383
Size: 625 450 308
Fragments arranged by size:
625 450 308
AlwNI CAG_nnn'CTG
Cuts at: 0 39 1121 1340 1383
Size: 39 1082 219 43
Fragments arranged by size:
1082 219 43 39
ApaI G_GGCC'C
Cuts at: 0 506 634 1383
Size: 506 128 749
Fragments arranged by size:
749 506 128
ApoI r'AATT_y
Cuts at: 0 22 1383
Size: 22 1361
AvaI C'yCGr_G
Cuts at: 0 133 1383
Size: 133 1250
BamHI G'GATC_C
Cuts at: 0 1109 1383
Size: 1109 274
BanI G'GyrC_C
Cuts at: 0 444 487 706 1139 1383
Size: 444 43 219 433 244
Fragments arranged by size:
444 433 244 219 43
BanII G_rGCy'C
Cuts at: 0 506 634 1212 1243 1325 1383
Size: 506 128 578 31 82 58
Fragments arranged by size:
578 506 128 82 58 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 282 489 1383
Size: 282 207 894
Fragments arranged by size:
894 282 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1147 1181 1383
Size: 1147 34 202
Fragments arranged by size:
1147 202 34
BglI GCCn_nnn'nGGC
Cuts at: 0 397 802 1383
Size: 397 405 581
Fragments arranged by size:
581 405 397
BglII A'GATC_T
Cuts at: 0 83 1383
Size: 83 1300
BlpI GC'TnA_GC
Cuts at: 0 232 1324 1383
Size: 232 1092 59
Fragments arranged by size:
1092 232 59
Bme1580I G_kGCm'C
Cuts at: 0 378 449 490 506 634 709 1144 1383
Size: 378 71 41 16 128 75 435 239
Fragments arranged by size:
435 378 239 128 75 71 41 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1350 1383
Size: 1350 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1383
Size: 147 1236
BsaAI yAC'GTr
Cuts at: 0 1077 1383
Size: 1077 306
BsaHI Gr'CG_yC
Cuts at: 0 287 1383
Size: 287 1096
BsaWI w'CCGG_w
Cuts at: 0 1091 1383
Size: 1091 292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1225 1255 1383
Size: 1225 30 128
Fragments arranged by size:
1225 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 838 1291 1383
Size: 112 726 453 92
Fragments arranged by size:
726 453 112 92
BseYI C'CCAG_C
Cuts at: 0 65 1328 1383
Size: 65 1263 55
Fragments arranged by size:
1263 65 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1383
Size: 193 1190
BsiHKAI G_wGCw'C
Cuts at: 0 1212 1325 1383
Size: 1212 113 58
Fragments arranged by size:
1212 113 58
BsmI GAATG_Cn'
Cuts at: 0 963 1383
Size: 963 420
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1378 1383
Size: 1378 5
Bsp1286I G_dGCh'C
Cuts at: 0 378 449 490 506 634 709 1144 1212
Size: 378 71 41 16 128 75 435 68
Cuts at: 1212 1243 1325 1383
Size: 31 82 58
Fragments arranged by size:
435 378 128 82 75 71 68 58
41 31 16
BspEI T'CCGG_A
Cuts at: 0 1091 1383
Size: 1091 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 926 1383
Size: 926 457
BsrFI r'CCGG_y
Cuts at: 0 560 1383
Size: 560 823
BssSI C'ACGA_G
Cuts at: 0 250 1383
Size: 250 1133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 39 366 1383
Size: 39 327 1017
Fragments arranged by size:
1017 327 39
BstEII G'GTnAC_C
Cuts at: 0 667 993 1383
Size: 667 326 390
Fragments arranged by size:
667 390 326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1383
Size: 627 756
BstYI r'GATC_y
Cuts at: 0 83 1109 1361 1383
Size: 83 1026 252 22
Fragments arranged by size:
1026 252 83 22
BtgI C'CryG_G
Cuts at: 0 167 710 791 1383
Size: 167 543 81 592
Fragments arranged by size:
592 543 167 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1150 1383
Size: 1150 233
BtsI GCAGTG_nn'
Cuts at: 0 407 1383
Size: 407 976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 149 184 1383
Size: 149 35 1199
Fragments arranged by size:
1199 149 35
EaeI y'GGCC_r
Cuts at: 0 2 389 794 1214 1383
Size: 2 387 405 420 169
Fragments arranged by size:
420 405 387 169 2
EarI CTCTTCn'nnn_
Cuts at: 0 646 1383
Size: 646 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 955 1383
Size: 955 428
EcoICRI GAG'CTC
Cuts at: 0 1210 1323 1383
Size: 1210 113 60
Fragments arranged by size:
1210 113 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 168 172 741 1011 1383
Size: 147 21 4 569 270 372
Fragments arranged by size:
569 372 270 147 21 4
EcoO109I rG'GnC_Cy
Cuts at: 0 72 503 631 846 974 1383
Size: 72 431 128 215 128 409
Fragments arranged by size:
431 409 215 128 128 72
EcoRV GAT'ATC
Cuts at: 0 512 1383
Size: 512 871
HaeII r_GCGC'y
Cuts at: 0 1013 1101 1383
Size: 1013 88 282
Fragments arranged by size:
1013 282 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1346 1378 1383
Size: 1346 32 5
Fragments arranged by size:
1346 32 5
MscI TGG'CCA
Cuts at: 0 4 391 1216 1383
Size: 4 387 825 167
Fragments arranged by size:
825 387 167 4
MslI CAynn'nnrTG
Cuts at: 0 625 1075 1383
Size: 625 450 308
Fragments arranged by size:
625 450 308
MspA1I CmG'CkG
Cuts at: 0 207 1328 1383
Size: 207 1121 55
Fragments arranged by size:
1121 207 55
NcoI C'CATG_G
Cuts at: 0 710 1383
Size: 710 673
NspI r_CATG'y
Cuts at: 0 160 1087 1383
Size: 160 927 296
Fragments arranged by size:
927 296 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 1340 1383
Size: 1340 43
PpuMI rG'GwC_Cy
Cuts at: 0 846 974 1383
Size: 846 128 409
Fragments arranged by size:
846 409 128
PspOMI G'GGCC_C
Cuts at: 0 502 630 1383
Size: 502 128 753
Fragments arranged by size:
753 502 128
PstI C_TGCA'G
Cuts at: 0 572 1383
Size: 572 811
PvuII CAG'CTG
Cuts at: 0 207 1328 1383
Size: 207 1121 55
Fragments arranged by size:
1121 207 55
SacI G_AGCT'C
Cuts at: 0 1212 1325 1383
Size: 1212 113 58
Fragments arranged by size:
1212 113 58
SfcI C'TryA_G
Cuts at: 0 568 1383
Size: 568 815
SphI G_CATG'C
Cuts at: 0 160 1383
Size: 160 1223
StyI C'CwwG_G
Cuts at: 0 540 710 1383
Size: 540 170 673
Fragments arranged by size:
673 540 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 875 1383
Size: 316 559 508
Fragments arranged by size:
559 508 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1101 1133 1383
Size: 1101 32 250
Fragments arranged by size:
1101 250 32
Tth111I GACn'n_nGTC
Cuts at: 0 855 1383
Size: 855 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 798 1224 1383
Size: 708 90 426 159
Fragments arranged by size:
708 426 159 90
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I
BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
HaeII Hin4I MscI MslI MspA1I NcoI NspI PflMI
PpuMI PspOMI PstI PvuII SacI SfcI SphI StyI
TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BspHI
BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI
DraI DraIII DrdI EagI EcoNI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TatI XbaI XhoI XmaI XmnI ZraI