ARFGEF1 ORF clone pFN21AB4231

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB4231

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Clone info.

Species

human

Clone Name

pFN21AB4231 Sequence (with vector) Restriction Map

Product ID

FHC12478

Flexi Clone PID

FXC12478

ORF Sequence

DNA sequence (5547 bp)
Protein sequence (1849 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB4231

Symbol

ARFGEF1

Alias : ARFGEP1, BIG1, P200

Description

ADP-ribosylation factor guanine nucleotide-exchange factor 1

Original Clone ID

oc01531

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5547 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1849 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6199.1 0 100.0 ARFGEF1 CCDS13411.1 0 75.0 ARFGEF2 CCDS32754.1 9.7e-32 43.9 CYTH1 CCDS42392.3 9.7e-32 43.9 CYTH1 CCDS86644.1 2.8e-31 51.6 CYTH1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000904 696 882 PF01369 SEC7-like IPR015403 1219 1303 PF09324 Domain of unknown function DUF1981 HMMSmart IPR000904 695 882 SM00222 SEC7-like ProfileScan IPR000904 691 880 PS50190 SEC7-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005251191 1849 604141 100.0 100.0 NP_006412 1849 604141 100.0 100.0 XP_005251192 1843 604141 99.7 100.0 XP_005251193 1800 604141 99.9 97.2 NP_006411 1785 605371,608097 75.0 99.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4231 Download

>KIBB4231  5547 bp
ATGTATGAGGGGAAGAAGACGAAGAACATGTTCCTGACCCGGGCTCTGGAGAAGATATTG
GCCGACAAGGAAGTGAAGAAGGCGCATCACTCCCAGCTGCGCAAAGCTTGCGAGGTGGCG
TTAGAGGAAATAAAAGCGGAAACTGAAAAACAGAGTCCTCCTCATGGAGAAGCAAAAGCT
GGATCAAGCACCCTTCCACCAGTGAAATCAAAGACAAATTTTATTGAAGCAGACAAGTAC
TTCTTGCCTTTTGAGTTGGCATGCCAGTCCAAATGTCCTCGCATAGTTAGTACATCTCTA
GATTGCTTACAGAAACTTATTGCTTATGGGCACTTGACTGGCAATGCTCCAGATAGTACA
ACACCAGGCAAAAAATTAATTGATAGAATTATTGAAACAATATGTGGCTGCTTTCAGGGC
CCTCAGACAGATGAAGGAGTTCAGCTGCAGATAATAAAGGCTTTACTTACTGCAGTAACA
TCACAACACATAGAAATTCATGAAGGGACTGTACTGCAAGCTGTGAGAACATGTTACAAT
ATCTACTTAGCAAGCAAAAATCTCATCAATCAGACAACAGCCAAAGCTACTCTCACTCAG
ATGCTAAATGTTATCTTTGCACGCATGGAAAACCAAGCATTACAGGAAGCAAAACAAATG
GAAAAAGAAAGGCATCGGCAGCATCATCATCTGTTACAGTCTCCAGTAAGCCATCACGAG
CCTGAATCACCTCAACTTAGATATTTGCCACCTCAGACTGTTGATCATATATCCCAAGAA
CACGAAGGGGACCTTGACCTCCATACAAATGATGTAGATAAAAGTCTTCAGGATGACACA
GAGCCTGAAAATGGATCTGATATTTCCAGTGCAGAAAATGAACAGACTGAAGCTGATCAG
GCAACTGCAGCTGAAACATTATCTAAAAATGAAGTGTTATATGACGGAGAAAACCATGAT
TGTGAGGAAAAGCCACAAGACATTGTACAGAACATTGTAGAAGAAATGGTGAACATTGTT
GTTGGAGATATGGGAGAAGGGACTACTATAAATGCAAGTGCAGATGGCAACATTGGAACT
ATAGAGGATGGTAGTGACAGTGAAAATATTCAAGCAAATGGAATTCCAGGAACACCAATT
TCTGTTGCATATACACCATCCTTACCTGATGATAGATTGTCAGTCTCTTCCAATGATACT
CAGGAATCTGGAAATTCTTCAGGACCTTCACCTGGTGCTAAGTTTTCCCACATTTTACAA
AAGGATGCCTTTCTAGTATTCAGGTCATTGTGTAAACTGTCAATGAAACCACTGTCAGAT
GGACCACCAGATCCAAAGTCTCATGAACTACGATCCAAGATTCTTTCATTGCAGTTACTT
CTATCCATTCTGCAGAATGCAGGACCTATTTTCAGGACAAATGAGATGTTTATTAATGCT
ATTAAGCAGTATCTTTGTGTTGCACTCTCAAAAAATGGAGTCTCATCTGTTCCAGAGGTT
TTTGAGCTTTCTCTTTCTATATTTCTTACTTTGTTGTCAAATTTCAAGACACATCTGAAG
ATGCAAATTGAGGTGTTCTTTAAAGAAATTTTCTTATACATTTTGGAAACTTCTACCAGC
TCATTTGATCACAAATGGATGGTTATTCAGACACTGACGAGGATTTGTGCAGATGCTCAG
AGTGTAGTGGATATTTATGTAAACTATGACTGTGACTTAAATGCAGCCAATATATTTGAA
AGACTAGTAAATGATCTATCAAAAATTGCTCAAGGAAGGGGCAGTCAAGAACTTGGTATG
AGTAATGTTCAGGAATTGAGCCTGAGGAAAAAAGGTTTAGAATGCTTAGTGTCGATTTTG
AAGTGTATGGTTGAATGGAGTAAGGATCAGTATGTGAATCCCAACTCCCAGACAACTCTT
GGTCAGGAAAAACCCTCAGAGCAAGAGATGAGTGAAATCAAACACCCTGAGACAATAAAC
AGATACGGAAGTTTAAATTCCCTGGAGTCAACATCATCATCAGGAATAGGCAGCTACAGT
ACACAGATGTCTGGCACTGATAATCCAGAACAATTTGAGGTCCTAAAGCAACAAAAAGAA
ATAATAGAACAAGGGATAGATTTATTTAATAAGAAACCAAAGAGAGGAATACAGTACCTC
CAAGAACAAGGGATGCTTGGCACCACACCTGAAGATATTGCCCAATTCTTACATCAAGAG
GAAAGATTAGACTCTACTCAAGTGGGTGAGTTCCTGGGAGATAATGATAAATTTAACAAA
GAAGTCATGTATGCATATGTGGACCAACATGACTTTTCAGGAAAAGACTTCGTTTCAGCC
CTTCGTATGTTTCTAGAAGGATTTCGTCTTCCAGGGGAAGCTCAGAAAATCGATCGATTA
ATGGAAAAATTTGCTGCAAGATACCTAGAATGCAACCAAGGACAAACTCTCTTTGCTAGT
GCGGATACAGCTTATGTTTTGGCTTATTCAATTATCATGTTGACCACAGACCTTCACAGT
CCACAGGTGAAAAATAAAATGACAAAGGAACAATACATTAAGATGAATAGAGGTATCAAT
GACAGTAAAGACCTTCCTGAAGAGTATCTATCAGCCATCTATAATGAAATAGCTGGGAAA
AAGATATCAATGAAAGAAACAAAAGAACTAACAATCCCTACAAAATCAAGTAAACAGAAT
GTAGCCAGTGAAAAACAAAGAAGACTTCTGTATAACTTAGAAATGGAGCAGATGGCCAAG
ACAGCTAAAGCACTCATGGAGGCCGTGAGCCATGTTCAGGCACCCTTTACAAGTGCAACA
CATTTGGAGCATGTGAGGCCCATGTTTAAGTTGGCTTGGACGCCTTTTCTGGCTGCATTC
AGTGTGGGTCTACAAGATTGTGATGATACTGAAGTAGCCTCTCTTTGCCTGGAAGGTATA
AGATGTGCAATCAGAATTGCATGCATTTTCAGCATTCAGCTGGAGAGAGATGCATATGTC
CAGGCACTAGCAAGATTTACCTTACTCACAGTGAGTTCCGGTATTACTGAAATGAAACAG
AAGAACATTGACACAATAAAAACACTTATCACAGTGGCTCATACAGATGGAAATTATTTA
GGAAATTCATGGCATGAGATTCTGAAGTGCATCAGTCAGTTGGAGCTGGCACAGCTTATA
GGAACTGGAGTGAAACCTCGATACATTTCTGGAACAGTGCGAGGCAGAGAAGGATCTCTT
ACTGGAACAAAAGATCAGGCTCCTGATGAATTTGTGGGTTTAGGGCTAGTTGGAGGAAAT
GTGGACTGGAAACAGATAGCAAGTATTCAGGAATCCATTGGAGAAACCAGTTCTCAGAGT
GTTGTTGTTGCTGTAGATAGAATATTCACAGGATCTACAAGGCTAGATGGAAATGCCATT
GTGGATTTTGTCCGTTGGCTCTGTGCTGTGTCTATGGATGAATTACTTTCCACGACACAC
CCAAGAATGTTTAGTCTACAAAAAATAGTAGAAATATCATATTACAACATGGGAAGAATA
AGATTACAGTGGTCTCGAATTTGGGAAGTTATTGGAGATCATTTTAATAAGGTTGGGTGT
AATCCTAATGAAGATGTAGCTATTTTTGCAGTAGACTCCTTGAGGCAGTTGTCAATGAAG
TTCTTAGAGAAAGGGGAGCTTGCTAACTTCAGATTCCAGAAGGATTTCTTAAGACCTTTT
GAACATATAATGAAACGGAACAGGTCTCCAACAATTCGAGATATGGTTGTACGGTGTATA
GCACAGATGGTTAATTCTCAAGCTGCTAACATTCGATCTGGATGGAAGAACATTTTCTCT
GTATTTCATCTAGCTGCATCTGATCAAGATGAAAGCATAGTGGAACTTGCATTCCAAACA
ACCGGGCACATTGTCACCCTTGTATTTGAAAAACACTTTCCAGCGACCATTGATTCTTTC
CAGGATGCAGTGAAGTGTTTGTCTGAATTTGCGTGCAATGCAGCTTTCCCAGACACAAGT
ATGGAAGCAATTCGACTTATTCGCCATTGTGCAAAATATGTGTCTGATAGACCTCAGGCT
TTCAAGGAATACACAAGCGATGATATGAACGTAGCACCTGAAGACAGGGTGTGGGTGAGA
GGATGGTTCCCAATTCTCTTTGAGTTATCCTGTATCATCAATAGATGCAAATTAGATGTA
AGAACCAGGGGTTTAACAGTAATGTTTGAAATAATGAAAACATATGGCCACACTTATGAG
AAACACTGGTGGCAGGATTTATTTAGAATTGTTTTCAGAATCTTTGACAATATGAAATTG
CCAGAACAACAGACAGAGAAAGCTGAATGGATGACAACAACTTGCAATCATGCACTTTAT
GCAATCTGTGATGTATTCACTCAGTATTTAGAAGTACTCAGTGATGTACTTTTGGATGAC
ATTTTTGCTCAGCTCTACTGGTGTGTGCAGCAAGACAATGAGCAGTTAGCGCGATCTGGT
ACAAACTGTTTAGAGAATGTTGTTATTCTGAATGGTGAAAAATTTACCCTAGAAATCTGG
GATAAAACTTGCAACTGCACACTGGATATCTTCAAAACCACAATCCCACATGCGCTGTTG
ACCTGGCGACCCAATTCTGGAGAAACTGCCCCCCCACCTCCATCTCCTGTAAGTGAAAAG
CCATTGGATACAATATCACAGAAGTCTGTAGATATTCATGATTCTATTCAACCAAGGTCT
GTGGATAACAGACCACAAGCACCACTGGTTTCTGCGTCTGCTGTTAATGAAGAAGTCAGC
AAAATTAAATCTACAGCAAAATTTCCAGAACAAAAATTGTTTGCTGCCCTGTTGATTAAA
TGTGTTGTGCAGCTGGAACTCATCCAGACTATCGACAACATTGTCTTCTTCCCAGCCACA
AGTAAGAAAGAAGATGCAGAAAACTTAGCTGCAGCACAGAGAGATGCGGTGGACTTTGAT
GTTCGCGTTGATACTCAAGACCAAGGAATGTACCGCTTTTTAACATCACAACAACTTTTT
AAGCTACTGGACTGCTTATTAGAGTCACATAGATTTGCAAAAGCGTTTAATTCCAACAAC
GAACAGAGGACTGCCCTGTGGAAAGCAGGATTCAAAGGCAAATCCAAGCCCAACCTTCTG
AAGCAGGAGACCAGCAGCCTGGCCTGTGGGCTGCGCATTCTCTTCCGGATGTACATGGAT
GAGAGCCGCGTTAGTGCCTGGGAGGAGGTCCAGCAGAGGCTTTTGAATGTCTGCAGTGAA
GCACTAAGTTACTTCCTCACTCTAACATCAGAAAGTCATCGAGAAGCCTGGACTAACTTA
CTGCTTTTGTTTCTAACTAAAGTTCTAAAGATAAGTGATAATAGGTTTAAAGCTCATGCA
TCATTCTACTACCCTCTCTTATGTGAAATTATGCAATTTGACTTGATTCCTGAACTTCGT
GCTGTTCTTAGAAGATTTTTTCTGCGAATCGGAGTAGTTTTTCAGATATCACAACCACCT
GAACAGGAACTTGGAATAAACAAGCAA

ORF Protein

Cloned ORF protein sequence for pFN21AB4231 Download

>KIBB4231  1849 aa
MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKA
GSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDST
TPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYN
IYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHE
PESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQ
ATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGT
IEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ
KDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINA
IKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTS
SFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGM
SNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETIN
RYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYL
QEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSA
LRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHS
PQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQN
VASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAF
SVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQ
KNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSL
TGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAI
VDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGC
NPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCI
AQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSF
QDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVR
GWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKL
PEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSG
TNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEK
PLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIK
CVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLF
KLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMD
ESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHA
SFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4231 Download

>pFN21AB4231  10252 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTATGAGGGGAAGAAGACGAAGAACATGTTCCT
GACCCGGGCTCTGGAGAAGATATTGGCCGACAAGGAAGTGAAGAAGGCGCATCACTCCCA
GCTGCGCAAAGCTTGCGAGGTGGCGTTAGAGGAAATAAAAGCGGAAACTGAAAAACAGAG
TCCTCCTCATGGAGAAGCAAAAGCTGGATCAAGCACCCTTCCACCAGTGAAATCAAAGAC
AAATTTTATTGAAGCAGACAAGTACTTCTTGCCTTTTGAGTTGGCATGCCAGTCCAAATG
TCCTCGCATAGTTAGTACATCTCTAGATTGCTTACAGAAACTTATTGCTTATGGGCACTT
GACTGGCAATGCTCCAGATAGTACAACACCAGGCAAAAAATTAATTGATAGAATTATTGA
AACAATATGTGGCTGCTTTCAGGGCCCTCAGACAGATGAAGGAGTTCAGCTGCAGATAAT
AAAGGCTTTACTTACTGCAGTAACATCACAACACATAGAAATTCATGAAGGGACTGTACT
GCAAGCTGTGAGAACATGTTACAATATCTACTTAGCAAGCAAAAATCTCATCAATCAGAC
AACAGCCAAAGCTACTCTCACTCAGATGCTAAATGTTATCTTTGCACGCATGGAAAACCA
AGCATTACAGGAAGCAAAACAAATGGAAAAAGAAAGGCATCGGCAGCATCATCATCTGTT
ACAGTCTCCAGTAAGCCATCACGAGCCTGAATCACCTCAACTTAGATATTTGCCACCTCA
GACTGTTGATCATATATCCCAAGAACACGAAGGGGACCTTGACCTCCATACAAATGATGT
AGATAAAAGTCTTCAGGATGACACAGAGCCTGAAAATGGATCTGATATTTCCAGTGCAGA
AAATGAACAGACTGAAGCTGATCAGGCAACTGCAGCTGAAACATTATCTAAAAATGAAGT
GTTATATGACGGAGAAAACCATGATTGTGAGGAAAAGCCACAAGACATTGTACAGAACAT
TGTAGAAGAAATGGTGAACATTGTTGTTGGAGATATGGGAGAAGGGACTACTATAAATGC
AAGTGCAGATGGCAACATTGGAACTATAGAGGATGGTAGTGACAGTGAAAATATTCAAGC
AAATGGAATTCCAGGAACACCAATTTCTGTTGCATATACACCATCCTTACCTGATGATAG
ATTGTCAGTCTCTTCCAATGATACTCAGGAATCTGGAAATTCTTCAGGACCTTCACCTGG
TGCTAAGTTTTCCCACATTTTACAAAAGGATGCCTTTCTAGTATTCAGGTCATTGTGTAA
ACTGTCAATGAAACCACTGTCAGATGGACCACCAGATCCAAAGTCTCATGAACTACGATC
CAAGATTCTTTCATTGCAGTTACTTCTATCCATTCTGCAGAATGCAGGACCTATTTTCAG
GACAAATGAGATGTTTATTAATGCTATTAAGCAGTATCTTTGTGTTGCACTCTCAAAAAA
TGGAGTCTCATCTGTTCCAGAGGTTTTTGAGCTTTCTCTTTCTATATTTCTTACTTTGTT
GTCAAATTTCAAGACACATCTGAAGATGCAAATTGAGGTGTTCTTTAAAGAAATTTTCTT
ATACATTTTGGAAACTTCTACCAGCTCATTTGATCACAAATGGATGGTTATTCAGACACT
GACGAGGATTTGTGCAGATGCTCAGAGTGTAGTGGATATTTATGTAAACTATGACTGTGA
CTTAAATGCAGCCAATATATTTGAAAGACTAGTAAATGATCTATCAAAAATTGCTCAAGG
AAGGGGCAGTCAAGAACTTGGTATGAGTAATGTTCAGGAATTGAGCCTGAGGAAAAAAGG
TTTAGAATGCTTAGTGTCGATTTTGAAGTGTATGGTTGAATGGAGTAAGGATCAGTATGT
GAATCCCAACTCCCAGACAACTCTTGGTCAGGAAAAACCCTCAGAGCAAGAGATGAGTGA
AATCAAACACCCTGAGACAATAAACAGATACGGAAGTTTAAATTCCCTGGAGTCAACATC
ATCATCAGGAATAGGCAGCTACAGTACACAGATGTCTGGCACTGATAATCCAGAACAATT
TGAGGTCCTAAAGCAACAAAAAGAAATAATAGAACAAGGGATAGATTTATTTAATAAGAA
ACCAAAGAGAGGAATACAGTACCTCCAAGAACAAGGGATGCTTGGCACCACACCTGAAGA
TATTGCCCAATTCTTACATCAAGAGGAAAGATTAGACTCTACTCAAGTGGGTGAGTTCCT
GGGAGATAATGATAAATTTAACAAAGAAGTCATGTATGCATATGTGGACCAACATGACTT
TTCAGGAAAAGACTTCGTTTCAGCCCTTCGTATGTTTCTAGAAGGATTTCGTCTTCCAGG
GGAAGCTCAGAAAATCGATCGATTAATGGAAAAATTTGCTGCAAGATACCTAGAATGCAA
CCAAGGACAAACTCTCTTTGCTAGTGCGGATACAGCTTATGTTTTGGCTTATTCAATTAT
CATGTTGACCACAGACCTTCACAGTCCACAGGTGAAAAATAAAATGACAAAGGAACAATA
CATTAAGATGAATAGAGGTATCAATGACAGTAAAGACCTTCCTGAAGAGTATCTATCAGC
CATCTATAATGAAATAGCTGGGAAAAAGATATCAATGAAAGAAACAAAAGAACTAACAAT
CCCTACAAAATCAAGTAAACAGAATGTAGCCAGTGAAAAACAAAGAAGACTTCTGTATAA
CTTAGAAATGGAGCAGATGGCCAAGACAGCTAAAGCACTCATGGAGGCCGTGAGCCATGT
TCAGGCACCCTTTACAAGTGCAACACATTTGGAGCATGTGAGGCCCATGTTTAAGTTGGC
TTGGACGCCTTTTCTGGCTGCATTCAGTGTGGGTCTACAAGATTGTGATGATACTGAAGT
AGCCTCTCTTTGCCTGGAAGGTATAAGATGTGCAATCAGAATTGCATGCATTTTCAGCAT
TCAGCTGGAGAGAGATGCATATGTCCAGGCACTAGCAAGATTTACCTTACTCACAGTGAG
TTCCGGTATTACTGAAATGAAACAGAAGAACATTGACACAATAAAAACACTTATCACAGT
GGCTCATACAGATGGAAATTATTTAGGAAATTCATGGCATGAGATTCTGAAGTGCATCAG
TCAGTTGGAGCTGGCACAGCTTATAGGAACTGGAGTGAAACCTCGATACATTTCTGGAAC
AGTGCGAGGCAGAGAAGGATCTCTTACTGGAACAAAAGATCAGGCTCCTGATGAATTTGT
GGGTTTAGGGCTAGTTGGAGGAAATGTGGACTGGAAACAGATAGCAAGTATTCAGGAATC
CATTGGAGAAACCAGTTCTCAGAGTGTTGTTGTTGCTGTAGATAGAATATTCACAGGATC
TACAAGGCTAGATGGAAATGCCATTGTGGATTTTGTCCGTTGGCTCTGTGCTGTGTCTAT
GGATGAATTACTTTCCACGACACACCCAAGAATGTTTAGTCTACAAAAAATAGTAGAAAT
ATCATATTACAACATGGGAAGAATAAGATTACAGTGGTCTCGAATTTGGGAAGTTATTGG
AGATCATTTTAATAAGGTTGGGTGTAATCCTAATGAAGATGTAGCTATTTTTGCAGTAGA
CTCCTTGAGGCAGTTGTCAATGAAGTTCTTAGAGAAAGGGGAGCTTGCTAACTTCAGATT
CCAGAAGGATTTCTTAAGACCTTTTGAACATATAATGAAACGGAACAGGTCTCCAACAAT
TCGAGATATGGTTGTACGGTGTATAGCACAGATGGTTAATTCTCAAGCTGCTAACATTCG
ATCTGGATGGAAGAACATTTTCTCTGTATTTCATCTAGCTGCATCTGATCAAGATGAAAG
CATAGTGGAACTTGCATTCCAAACAACCGGGCACATTGTCACCCTTGTATTTGAAAAACA
CTTTCCAGCGACCATTGATTCTTTCCAGGATGCAGTGAAGTGTTTGTCTGAATTTGCGTG
CAATGCAGCTTTCCCAGACACAAGTATGGAAGCAATTCGACTTATTCGCCATTGTGCAAA
ATATGTGTCTGATAGACCTCAGGCTTTCAAGGAATACACAAGCGATGATATGAACGTAGC
ACCTGAAGACAGGGTGTGGGTGAGAGGATGGTTCCCAATTCTCTTTGAGTTATCCTGTAT
CATCAATAGATGCAAATTAGATGTAAGAACCAGGGGTTTAACAGTAATGTTTGAAATAAT
GAAAACATATGGCCACACTTATGAGAAACACTGGTGGCAGGATTTATTTAGAATTGTTTT
CAGAATCTTTGACAATATGAAATTGCCAGAACAACAGACAGAGAAAGCTGAATGGATGAC
AACAACTTGCAATCATGCACTTTATGCAATCTGTGATGTATTCACTCAGTATTTAGAAGT
ACTCAGTGATGTACTTTTGGATGACATTTTTGCTCAGCTCTACTGGTGTGTGCAGCAAGA
CAATGAGCAGTTAGCGCGATCTGGTACAAACTGTTTAGAGAATGTTGTTATTCTGAATGG
TGAAAAATTTACCCTAGAAATCTGGGATAAAACTTGCAACTGCACACTGGATATCTTCAA
AACCACAATCCCACATGCGCTGTTGACCTGGCGACCCAATTCTGGAGAAACTGCCCCCCC
ACCTCCATCTCCTGTAAGTGAAAAGCCATTGGATACAATATCACAGAAGTCTGTAGATAT
TCATGATTCTATTCAACCAAGGTCTGTGGATAACAGACCACAAGCACCACTGGTTTCTGC
GTCTGCTGTTAATGAAGAAGTCAGCAAAATTAAATCTACAGCAAAATTTCCAGAACAAAA
ATTGTTTGCTGCCCTGTTGATTAAATGTGTTGTGCAGCTGGAACTCATCCAGACTATCGA
CAACATTGTCTTCTTCCCAGCCACAAGTAAGAAAGAAGATGCAGAAAACTTAGCTGCAGC
ACAGAGAGATGCGGTGGACTTTGATGTTCGCGTTGATACTCAAGACCAAGGAATGTACCG
CTTTTTAACATCACAACAACTTTTTAAGCTACTGGACTGCTTATTAGAGTCACATAGATT
TGCAAAAGCGTTTAATTCCAACAACGAACAGAGGACTGCCCTGTGGAAAGCAGGATTCAA
AGGCAAATCCAAGCCCAACCTTCTGAAGCAGGAGACCAGCAGCCTGGCCTGTGGGCTGCG
CATTCTCTTCCGGATGTACATGGATGAGAGCCGCGTTAGTGCCTGGGAGGAGGTCCAGCA
GAGGCTTTTGAATGTCTGCAGTGAAGCACTAAGTTACTTCCTCACTCTAACATCAGAAAG
TCATCGAGAAGCCTGGACTAACTTACTGCTTTTGTTTCTAACTAAAGTTCTAAAGATAAG
TGATAATAGGTTTAAAGCTCATGCATCATTCTACTACCCTCTCTTATGTGAAATTATGCA
ATTTGACTTGATTCCTGAACTTCGTGCTGTTCTTAGAAGATTTTTTCTGCGAATCGGAGT
AGTTTTTCAGATATCACAACCACCTGAACAGGAACTTGGAATAAACAAGCAAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT