Restriction map for
pFN21AB4231
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspHI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BglI
BglII BlnI BsaAI BsaBI BsiHKAI
BsiWI BsmBI BspMI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FseI HaeII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I StuI SwaI Tth111I
Van91I XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccIII 5204 TCCGGA T^CCGGA
AcyI 2859 GACGCC G[GA]^CG[CT]C
AflII 3708 CTTAAG C^TTAAG
ApaI 417 GGGCCC GGGCC^C
AvaI 38 CCCGGG C^[CT]CG[GA]G
BfrI 3708 CTTAAG C^TTAAG
BlpI 4447 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4447 GCTCAGC GC^T[ACGT]AGC
BsaHI 2859 GACGCC G[GA]^CG[CT]C
BseAI 5204 TCCGGA T^CCGGA
BsiEI 2391 CGATCG CG[GA][CT]^CG
BsoBI 38 CCCGGG C^[CT]CG[GA]G
BspEI 5204 TCCGGA T^CCGGA
BssSI 715 CACGAG CACGAG(-5/-1)
BstXI 5171 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4447 GCTCAGC GC^T[ACGT]AGC
DraIII 1229 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4894 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1121 GAATTC G^AATTC
Hin1I 2859 GACGCC G[GA]^CG[CT]C
HindIII 104 AAGCTT A^AGCTT
MaeII 4109 ACGT A^CGT
MroI 5204 TCCGGA T^CCGGA
PvuI 2391 CGATCG CGAT^CG
SexAI 1230 ACCTGGT A^CC[AT]GGT
SmaI 38 CCCGGG CCC^GGG
SpeI 1743 ACTAGT A^CTAGT
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AflIII 26 ACATGT A^C[GA][CT]GT
529 ACATGT
AlwNI 839 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1648 CAGACACTG
AocI 1821 CCTGAGG CC^T[ACGT]AGG
4072 CCTCAGG
AviII 98 TGCGCA TGC^GCA
5192 TGCGCA
BalI 2753 TGGCCA TGG^CCA
4245 TGGCCA
BanI 2179 GGCACC G^G[CT][GA]CC
2799 GGCACC
BanII 41 GGGCTC G[GA]GC[CT]^C
417 GGGCCC
BciVI 2463 GGATAC GTATCC(6/5)
4686 GGATAC
BsaWI 3037 TCCGGT [AT]^CCGG[AT]
5204 TCCGGA
BseRI 158 CTCCTC GAGGAG(10/8)
5242 GAGGAG
Bsp1407I 984 TGTACA T^GTACA
5210 TGTACA
BspDI 2389 ATCGAT AT^CGAT
2393 ATCGAT
BspLU11I 26 ACATGT A^CATGT
529 ACATGT
BsrGI 984 TGTACA T^GTACA
5210 TGTACA
BstAPI 101 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4372 GCACTTTATGC
Bsu36I 1821 CCTGAGG CC^T[ACGT]AGG
4072 CCTCAGG
ClaI 2389 ATCGAT AT^CGAT
2393 ATCGAT
Eco81I 1821 CCTGAGG CC^T[ACGT]AGG
4072 CCTCAGG
FspI 98 TGCGCA TGC^GCA
5192 TGCGCA
HgaI 2859 GACGC GACGC(5/10)
4774 GCGTC
MluNI 2753 TGGCCA TGG^CCA
4245 TGGCCA
MscI 2753 TGGCCA TGG^CCA
4245 TGGCCA
ScaI 236 AGTACT AGT^ACT
4413 AGTACT
SphI 259 GCATGC GCATG^C
2959 GCATGC
SspBI 984 TGTACA T^GTACA
5210 TGTACA
SspI 1105 AATATT AAT^ATT
3381 AATATT
XbaI 297 TCTAGA T^CTAGA
2352 TCTAGA
XcmI 1908 CCAGACAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2840 CCATGTTTAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoRV EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI