(Circular) (Six-base) MAPSORT of: pFN21AB4231.seq Check: 7756 from: 1 to: 10252 pFN21AB4231 10252 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8062 278 Size: 53 83 186 936 6526 2468 Fragments arranged by size: 6526 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4894 5500 5637 7595 1937 Size: 2957 606 137 1958 4594 Fragments arranged by size: 4594 2957 1958 606 137 Acc65I G'GTAC_C Cuts at: 7573 7573 Size: 10252 AclI AA'CG_TT Cuts at: 1563 8379 8752 1563 Size: 6816 373 3063 Fragments arranged by size: 6816 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2816 2913 3207 3581 4215 4623 4935 5168 Size: 1184 97 294 374 634 408 312 233 Cuts at: 5168 5677 6144 7184 8315 9699 9967 1632 Size: 509 467 1040 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1184 1131 1040 634 509 467 408 374 312 294 268 233 97 AflII C'TTAA_G Cuts at: 820 1017 5713 820 Size: 197 4696 5359 Fragments arranged by size: 5359 4696 197 AflIII A'CryG_T Cuts at: 1203 2031 2534 9151 10128 1203 Size: 828 503 6617 977 1327 Fragments arranged by size: 6617 1327 977 828 503 AgeI A'CCGG_T Cuts at: 9083 9083 Size: 10252 AhdI GACnn_n'nnGTC Cuts at: 8981 8981 Size: 10252 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10252 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4662 4694 5880 5912 4662 Size: 32 1186 32 9002 Fragments arranged by size: 9002 1186 32 32 AlwNI CAG_nnn'CTG Cuts at: 2849 3658 7995 9567 2849 Size: 809 4337 1572 3534 Fragments arranged by size: 4337 3534 1572 809 ApaI G_GGCC'C Cuts at: 2426 2426 Size: 10252 ApaLI G'TGCA_C Cuts at: 8309 9465 8309 Size: 1156 9096 ApoI r'AATT_y Cuts at: 1493 2221 2499 3126 3217 3544 3591 4000 4274 Size: 728 278 627 91 327 47 409 274 Cuts at: 4274 4412 5128 5273 5562 5990 6545 6824 7561 Size: 138 716 145 289 428 555 279 737 Cuts at: 7561 7787 7939 10048 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 737 728 716 627 555 428 409 327 289 279 278 274 226 152 145 138 91 47 AseI AT'TA_AT Cuts at: 160 1032 2381 3438 4403 8806 160 Size: 872 1349 1057 965 4403 1606 Fragments arranged by size: 4403 1606 1349 1057 965 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10252 AvaI C'yCGr_G Cuts at: 1870 1943 2043 7577 1870 Size: 73 100 5534 4545 Fragments arranged by size: 5534 4545 100 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3979 4012 4902 4935 3979 Size: 33 890 33 9296 Fragments arranged by size: 9296 890 33 33 BamHI G'GATC_C Cuts at: 7582 7949 7582 Size: 367 9885 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4184 4804 7573 9028 618 Size: 325 217 627 2397 620 2769 1455 1842 Fragments arranged by size: 2769 2397 1842 1455 627 620 325 217 BanII G_rGCy'C Cuts at: 1391 2050 2426 7571 1391 Size: 659 376 5145 4072 Fragments arranged by size: 5145 4072 659 376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2027 2822 4364 4732 6132 6901 928 Size: 971 128 795 1542 368 1400 769 4279 Fragments arranged by size: 4279 1542 1400 971 795 769 368 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8443 8477 1903 Size: 34 6506 34 3678 Fragments arranged by size: 6506 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4462 6685 8144 9354 4462 Size: 2223 1459 1210 5360 Fragments arranged by size: 5360 2223 1459 1210 BclI T'GATC_A Cuts at: 1548 2767 2899 3631 5866 1548 Size: 1219 132 732 2235 5934 Fragments arranged by size: 5934 2235 1219 732 132 BfrBI ATG'CAT Cuts at: 4298 4968 4997 7403 4298 Size: 670 29 2406 7147 Fragments arranged by size: 7147 2406 670 29 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8863 136 Size: 107 122 71 631 605 7191 1525 Fragments arranged by size: 7191 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 10247 10247 Size: 10252 BlpI GC'TnA_GC Cuts at: 6453 7669 6453 Size: 1216 9036 Bme1580I G_kGCm'C Cuts at: 1790 2337 2426 5913 8313 9469 1790 Size: 547 89 3487 2400 1156 2573 Fragments arranged by size: 3487 2573 2400 1156 547 89 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10252 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7983 8941 442 Size: 416 53 83 399 24 6566 958 1753 Fragments arranged by size: 6566 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10252 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2181 2181 Size: 10252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2337 2691 4028 5006 5211 6663 8912 Size: 833 265 354 1337 978 205 1452 2249 Cuts at: 8912 1239 Size: 2579 Fragments arranged by size: 2579 2249 1452 1337 978 833 354 265 205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3759 4227 5665 5787 6984 8377 9242 9540 3759 Size: 468 1438 122 1197 1393 865 298 4471 Fragments arranged by size: 4471 1438 1393 1197 865 468 298 122 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5562 5754 7166 8915 882 Size: 617 4063 192 1412 1749 2219 Fragments arranged by size: 4063 2219 1749 1412 617 192 BsaAI yAC'GTr Cuts at: 493 1204 8043 493 Size: 711 6839 2702 Fragments arranged by size: 6839 2702 711 BsaBI GATnn'nnATC Cuts at: 10246 10246 Size: 10252 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4865 8059 8441 9076 Size: 53 83 186 936 3332 3194 382 635 Cuts at: 9076 275 Size: 1451 Fragments arranged by size: 3332 3194 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5042 7209 8685 9083 9357 9504 9915 5042 Size: 2167 1476 398 274 147 411 5379 Fragments arranged by size: 5379 2167 1476 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4146 4176 5289 5319 1178 Size: 30 165 30 2743 30 1113 30 6111 Fragments arranged by size: 6111 2743 1113 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2154 7262 1186 Size: 968 5108 4176 Fragments arranged by size: 5108 4176 968 BseYI C'CCAG_C Cuts at: 2097 4637 6916 9455 9951 2097 Size: 2540 2279 2539 496 2398 Fragments arranged by size: 2540 2539 2398 2279 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2895 3084 3693 6491 6565 6893 1163 Size: 573 1159 189 609 2798 74 328 4522 Fragments arranged by size: 4522 2798 1159 609 573 328 189 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4399 7702 8463 9491 1540 Size: 275 186 2398 3303 761 1028 2301 Fragments arranged by size: 3303 2398 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 8313 8398 9469 8313 Size: 85 1071 9096 Fragments arranged by size: 9096 1071 85 BsmI GAATG_Cn' Cuts at: 3406 3851 4439 4880 4977 5894 7200 7763 7856 Size: 445 588 441 97 917 1306 563 93 Cuts at: 7856 3406 Size: 5802 Fragments arranged by size: 5802 1306 917 588 563 445 441 97 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2337 2426 5913 7571 8313 8398 Size: 399 260 287 89 3487 1658 742 85 Cuts at: 8398 9469 1391 Size: 1071 2174 Fragments arranged by size: 3487 2174 1658 1071 742 399 287 260 89 85 BspEI T'CCGG_A Cuts at: 7209 9915 7209 Size: 2706 7546 BspHI T'CATG_A Cuts at: 2503 3346 6721 8141 9871 2503 Size: 843 3375 1420 1730 2884 Fragments arranged by size: 3375 2884 1730 1420 843 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 7607 844 Size: 6763 3489 BsrBI CCG'CTC Cuts at: 1394 1823 8139 1394 Size: 429 6316 3507 Fragments arranged by size: 6316 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2353 3371 6007 8747 8921 65 Size: 1183 1105 1018 2636 2740 174 1396 Fragments arranged by size: 2740 2636 1396 1183 1105 1018 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8896 9083 1700 Size: 110 7086 187 2869 Fragments arranged by size: 7086 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2989 7215 96 Size: 2893 4226 3133 Fragments arranged by size: 4226 3133 2893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10252 BssSI C'ACGA_G Cuts at: 2720 8312 9324 2720 Size: 5592 1012 3648 Fragments arranged by size: 5592 3648 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2112 6383 794 Size: 1318 4271 4663 Fragments arranged by size: 4663 4271 1318 BstBI TT'CG_AA Cuts at: 9056 9056 Size: 10252 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10252 BstXI CCAn_nnnn'nTGG Cuts at: 7183 7183 Size: 10252 BstYI r'GATC_y Cuts at: 1972 2858 3334 5237 5396 7582 7949 8334 8351 Size: 886 476 1903 159 2186 367 385 17 Cuts at: 8351 9803 9889 9901 10247 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2186 1977 1903 1452 886 476 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3827 6078 3827 Size: 2251 8001 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 9700 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6117 487 Size: 683 56 4891 4622 Fragments arranged by size: 4891 4622 683 56 BtsI GCAGTG_nn' Cuts at: 5979 7285 7768 8562 8582 5979 Size: 1306 483 794 20 7649 Fragments arranged by size: 7649 1306 794 483 20 ClaI AT'CG_AT Cuts at: 1554 4395 4399 1554 Size: 2841 4 7407 Fragments arranged by size: 7407 2841 4 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6726 6761 529 Size: 35 6162 35 4020 Fragments arranged by size: 6162 4020 35 35 DraI TTT'AAA Cuts at: 3586 3999 7393 7556 3586 Size: 413 3394 163 6282 Fragments arranged by size: 6282 3394 413 163 DraIII CAC_nnn'GTG Cuts at: 3239 3239 Size: 10252 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6905 9259 809 Size: 507 5589 2354 1802 Fragments arranged by size: 5589 2354 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 4758 6250 Size: 54 1425 50 275 18 234 2694 1492 Cuts at: 6250 7699 8588 10217 8 Size: 1449 889 1629 43 Fragments arranged by size: 2694 1629 1492 1449 1425 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7699 1537 Size: 275 5887 4090 Fragments arranged by size: 5887 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3196 4599 7211 8182 1353 Size: 1843 1403 2612 971 3423 Fragments arranged by size: 3423 2612 1843 1403 971 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8839 9213 9359 387 Size: 1064 138 7250 374 146 1280 Fragments arranged by size: 7250 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2337 2691 2816 2913 3207 3581 Size: 393 440 265 354 125 97 294 374 Cuts at: 3581 4028 4215 4623 4935 5006 5168 5211 5677 Size: 447 187 408 312 71 162 43 466 Cuts at: 5677 6144 6663 7184 8315 8912 9699 9967 1239 Size: 467 519 521 1131 597 787 268 1524 Fragments arranged by size: 1524 1131 787 597 521 519 467 466 447 440 408 393 374 354 312 294 268 265 187 162 125 97 71 43 EcoO109I rG'GnC_Cy Cuts at: 2422 2423 2794 3227 3407 4084 2422 Size: 1 371 433 180 677 8590 Fragments arranged by size: 8590 677 433 371 180 1 EcoRI G'AATT_C Cuts at: 3126 7561 7939 3126 Size: 4435 378 5439 Fragments arranged by size: 5439 4435 378 EcoRV GAT'ATC Cuts at: 4650 6592 7512 4650 Size: 1942 920 7390 Fragments arranged by size: 7390 1942 920 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2813 3289 4196 5258 5699 5728 2263 Size: 550 476 907 1062 441 29 6787 Fragments arranged by size: 6787 1062 907 550 476 441 29 FspI TGC'GCA Cuts at: 2105 7199 8758 2105 Size: 5094 1559 3599 Fragments arranged by size: 5094 3599 1559 HaeII r_GCGC'y Cuts at: 1678 9399 1678 Size: 7721 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 2714 2746 2752 Size: 32 135 32 460 32 650 32 6 Cuts at: 2752 2759 2784 2791 3167 3171 3199 3203 3326 Size: 7 25 7 376 4 28 4 123 Cuts at: 3326 3358 3874 3906 4001 4033 4437 4469 4598 Size: 32 516 32 95 32 404 32 129 Cuts at: 4598 4630 4938 4970 6683 6715 8893 8925 8967 Size: 32 308 32 1713 32 2178 32 42 Cuts at: 8967 8999 1373 Size: 32 2626 Fragments arranged by size: 2626 2178 1713 650 516 460 404 376 308 135 129 123 95 42 32 32 32 32 32 32 32 32 32 32 32 32 32 28 25 7 7 6 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 4014 4506 6624 7596 7842 669 Size: 988 281 2076 492 2118 972 246 3079 Fragments arranged by size: 3079 2118 2076 988 972 492 281 246 HindIII A'AGCT_T Cuts at: 748 2109 748 Size: 1361 8891 HpaI GTT'AAC Cuts at: 7842 7842 Size: 10252 KpnI G_GTAC'C Cuts at: 7577 7577 Size: 10252 MfeI C'AATT_G Cuts at: 7851 7851 Size: 10252 MluI A'CGCG_T Cuts at: 10128 10128 Size: 10252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3007 5145 5275 5777 7122 9366 9550 1305 Size: 1702 2138 130 502 1345 2244 184 2007 Fragments arranged by size: 2244 2138 2007 1702 1345 502 184 130 MscI TGG'CCA Cuts at: 10 64 1489 1832 4760 6252 10 Size: 54 1425 343 2928 1492 4010 Fragments arranged by size: 4010 2928 1492 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 2811 2964 4565 5109 6023 6258 Size: 648 1437 208 153 1601 544 914 235 Cuts at: 6258 8210 8569 8728 518 Size: 1952 359 159 2042 Fragments arranged by size: 2042 1952 1601 1437 914 648 544 359 235 208 159 153 MspA1I CmG'CkG Cuts at: 1606 2101 2449 2915 4984 6877 7668 8345 9493 Size: 495 348 466 2069 1893 791 677 1148 Cuts at: 9493 9738 1606 Size: 245 2120 Fragments arranged by size: 2120 2069 1893 1148 791 677 495 466 348 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 9700 NdeI CA'TA_TG Cuts at: 387 4300 4999 6247 387 Size: 3913 699 1248 4392 Fragments arranged by size: 4392 3913 1248 699 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10252 NotI GC'GGCC_GC Cuts at: 7699 7699 Size: 10252 NsiI A_TGCA'T Cuts at: 4300 4970 4999 7405 4300 Size: 670 29 2406 7147 Fragments arranged by size: 7147 2406 670 29 NspI r_CATG'y Cuts at: 1132 1229 2035 2268 2538 4838 4968 6617 7610 Size: 97 806 233 270 2300 130 1649 993 Cuts at: 7610 9155 1132 Size: 1545 2229 Fragments arranged by size: 2300 2229 1649 1545 993 806 270 233 130 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10252 PciI A'CATG_T Cuts at: 2031 2534 9151 2031 Size: 503 6617 3132 Fragments arranged by size: 6617 3132 503 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10252 PfoI T'CCnGG_A Cuts at: 3130 5964 3130 Size: 2834 7418 PmeI GTTT'AAAC Cuts at: 7556 7556 Size: 10252 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5825 5857 8321 8353 9834 9866 5825 Size: 32 2464 32 1481 32 6211 Fragments arranged by size: 6211 2464 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 3227 3407 4084 2794 Size: 433 180 677 8962 Fragments arranged by size: 8962 677 433 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10252 PsiI TTA'TAA Cuts at: 7822 7822 Size: 10252 PspOMI G'GGCC_C Cuts at: 2422 2422 Size: 10252 PstI C_TGCA'G Cuts at: 830 2454 2479 2914 3399 6958 7280 7604 830 Size: 1624 25 435 485 3559 322 324 3478 Fragments arranged by size: 3559 3478 1624 485 435 324 322 25 PvuI CG_AT'CG Cuts at: 2001 4399 2001 Size: 2398 7854 PvuII CAG'CTG Cuts at: 2101 2449 2915 4984 6877 2101 Size: 348 466 2069 1893 5476 Fragments arranged by size: 5476 2069 1893 466 348 SalI G'TCGA_C Cuts at: 1936 7594 1936 Size: 5658 4594 SbfI CC_TGCA'GG Cuts at: 7604 7604 Size: 10252 ScaI AGT'ACT Cuts at: 2243 6420 8500 10212 2243 Size: 4177 2080 1712 2283 Fragments arranged by size: 4177 2283 2080 1712 SexAI A'CCwGG_T Cuts at: 3235 3235 Size: 10252 SfcI C'TryA_G Cuts at: 826 1045 2450 2475 2910 3084 3395 4039 5376 Size: 219 1405 25 435 174 311 644 1337 Cuts at: 5376 6711 6816 6954 7276 7600 8735 9416 9607 Size: 1335 105 138 322 324 1135 681 191 Cuts at: 9607 826 Size: 1471 Fragments arranged by size: 1471 1405 1337 1335 1135 681 644 435 324 322 311 219 191 174 138 105 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10252 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10252 SmaI CCC'GGG Cuts at: 1872 2045 7579 1872 Size: 173 5534 4545 Fragments arranged by size: 5534 4545 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 3774 4242 5644 5713 5802 6999 Size: 197 926 1831 468 1402 69 89 1197 Cuts at: 6999 8356 9257 9519 820 Size: 1357 901 262 1553 Fragments arranged by size: 1831 1553 1402 1357 1197 926 901 468 262 197 89 69 SnaBI TAC'GTA Cuts at: 493 8043 493 Size: 7550 2702 SpeI A'CTAG_T Cuts at: 152 3748 152 Size: 3596 6656 SphI G_CATG'C Cuts at: 1132 2268 4968 7610 1132 Size: 1136 2700 2642 3774 Fragments arranged by size: 3774 2700 2642 1136 SspI AAT'ATT Cuts at: 5 52 3112 5388 8176 5 Size: 47 3060 2276 2788 2081 Fragments arranged by size: 3060 2788 2276 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10252 StyI C'CwwG_G Cuts at: 513 1065 4441 6737 7006 7691 513 Size: 552 3376 2296 269 685 3074 Fragments arranged by size: 3376 3074 2296 685 552 269 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4238 5499 5589 6127 8279 8296 8449 Size: 1255 2658 1261 90 538 2152 17 153 Cuts at: 8449 8634 9079 325 Size: 185 445 1498 Fragments arranged by size: 2658 2152 1498 1261 1255 538 445 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2294 2360 Size: 274 80 33 51 1443 264 53 66 Cuts at: 2360 2515 2989 4043 6418 6430 7215 8498 10210 Size: 155 474 1054 2375 12 785 1283 1712 Cuts at: 10210 96 Size: 138 Fragments arranged by size: 2375 1712 1443 1283 1054 785 474 274 264 155 138 80 66 53 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4051 4083 5178 5210 5289 5321 10212 10244 4051 Size: 32 1095 32 79 32 4891 32 4059 Fragments arranged by size: 4891 4059 1095 79 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10252 XbaI T'CTAG_A Cuts at: 2302 4357 7588 2302 Size: 2055 3231 4966 Fragments arranged by size: 4966 3231 2055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3920 4852 3920 Size: 932 9320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10252 XmaI C'CCGG_G Cuts at: 1870 2043 7577 1870 Size: 173 5534 4545 Fragments arranged by size: 5534 4545 173 XmnI GAAnn'nnTTC Cuts at: 2033 2442 3111 3220 4365 5708 5837 6033 8381 Size: 409 669 109 1145 1343 129 196 2348 Cuts at: 8381 2033 Size: 3904 Fragments arranged by size: 3904 2348 1343 1145 669 409 196 129 109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8060 276 Size: 53 83 186 936 6526 2468 Fragments arranged by size: 6526 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI