KCNJ10 ORF clone pFN21AB3644

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Halotag ORF Clone pFN21AB3644

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Clone info.

Species

human

Clone Name

pFN21AB3644 Sequence (with vector) Restriction Map

Product ID

FHC01307

Flexi Clone PID

FXC01307

ORF Sequence

DNA sequence (1137 bp)
Protein sequence (379 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384828]

Gene

KIBB3644

Symbol

KCNJ10

Alias : BIRK-10, KCNJ13-PEN, KIR1.2, KIR4.1, SESAME

Description

potassium channel, inwardly rectifying subfamily J, member 10

Original Clone ID

hk00137

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1137 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 379 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1193.1 4e-173 100.0 KCNJ10 CCDS13656.1 2.9e-102 62.9 KCNJ15 CCDS8477.1 4.4e-80 53.4 KCNJ1 CCDS8476.1 4.7e-80 53.4 KCNJ1 CCDS11219.1 8.2e-63 45.0 KCNJ12 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003269 1 14 PR01322 Potassium channel IPR003269 15 25 PR01322 Potassium channel IPR003269 41 50 PR01322 Potassium channel IPR013521 64 90 PR01320 Potassium channel IPR013521 118 140 PR01320 Potassium channel IPR003269 186 194 PR01322 Potassium channel IPR003269 227 234 PR01322 Potassium channel IPR013521 247 265 PR01320 Potassium channel IPR013521 277 297 PR01320 Potassium channel IPR013521 298 315 PR01320 Potassium channel IPR003269 312 320 PR01322 Potassium channel IPR013521 323 337 PR01320 Potassium channel IPR003269 331 339 PR01322 Potassium channel IPR003269 341 350 PR01322 Potassium channel IPR003269 364 372 PR01322 Potassium channel HMMPfam IPR013521 32 367 PF01007 Potassium channel
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 71 FSATFAGTWFLFGVVWYLVAVAH 93 PRIMARY 23 2 141 PLAIVLLIAQLVLTTILEIFITG 163 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002232 379 274600,600791,602208,612780 100.0 100.0 XP_011527863 375 602106 62.9 95.8 XP_011527862 375 602106 62.9 95.8 NP_733933 375 602106 62.9 95.8 NP_001263366 375 602106 62.9 95.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3644 Download

>KIBB3644  1137 bp
ATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGAAAGCCGGCCCCTA
ATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCGCAGCAACGTGAGA
ATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTGGACAACCTTCATT
GACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGCAGGCACATGGTTC
CTCTTTGGCGTGGTGTGGTATCTGGTAGCTGTGGCACATGGGGACCTGCTGGAGCTGGAC
CCCCCGGCCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCACTGGAGCCTTCCTC
TTCTCCCTTGAATCCCAAACCACCATTGGCTATGGCTTCCGCTACATCAGTGAGGAATGT
CCACTGGCCATTGTGCTTCTTATTGCCCAGCTGGTGCTCACCACCATCCTGGAAATCTTC
ATCACAGGTACCTTCCTGGCGAAGATTGCCCGGCCCAAGAAGCGGGCTGAGACCATTCGT
TTCAGCCAGCATGCAGTTGTGGCCTCCCACAATGGCAAGCCCTGCCTCATGATCCGAGTT
GCCAATATGCGCAAAAGCCTCCTCATTGGCTGCCAGGTGACAGGAAAACTGCTTCAGACC
CACCAAACCAAGGAAGGGGAGAACATCCGGCTCAACCAGGTCAATGTGACTTTCCAAGTA
GACACAGCCTCTGACAGCCCCTTCCTTATTCTACCCCTTACCTTCTATCATGTGGTAGAT
GAGACCAGTCCCTTGAAAGATCTCCCTCTTCGCAGTGGTGAGGGTGACTTTGAGCTGGTG
CTGATCCTAAGTGGGACAGTGGAGTCCACCAGTGCCACCTGTCAGGTGCGCACTTCCTAC
CTGCCAGAGGAGATCCTTTGGGGCTACGAGTTCACACCTGCCATCTCACTGTCAGCCAGT
GGTAAATACATAGCTGACTTTAGCCTTTTTGACCAAGTTGTGAAAGTGGCCTCTCCTAGT
GGCCTCCGTGACAGCACTGTACGCTACGGAGACCCTGAAAAGCTCAAGTTGGAGGAGTCA
TTAAGGGAGCAAGCTGAGAAGGAGGGCAGTGCCCTTAGTGTGCGCATCAGCAATGTC

ORF Protein

Cloned ORF protein sequence for pFN21AB3644 Download

>KIBB3644  379 aa
MTSVAKVYYSQTTQTESRPLMGPGIRRRRVLTKDGRSNVRMEHIADKRFLYLKDLWTTFI
DMQWRYKLLLFSATFAGTWFLFGVVWYLVAVAHGDLLELDPPANHTPCVVQVHTLTGAFL
FSLESQTTIGYGFRYISEECPLAIVLLIAQLVLTTILEIFITGTFLAKIARPKKRAETIR
FSQHAVVASHNGKPCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTFQV
DTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELVLILSGTVESTSATCQVRTSY
LPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEES
LREQAEKEGSALSVRISNV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3644 Download

>pFN21AB3644  5842 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACGTCAGTTGCCAAGGTGTATTACAGTCAGAC
CACTCAGACAGAAAGCCGGCCCCTAATGGGCCCAGGGATACGACGGCGGAGAGTCCTGAC
AAAAGATGGTCGCAGCAACGTGAGAATGGAGCACATTGCCGACAAGCGCTTCCTCTACCT
CAAGGACCTGTGGACAACCTTCATTGACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTC
TGCGACCTTTGCAGGCACATGGTTCCTCTTTGGCGTGGTGTGGTATCTGGTAGCTGTGGC
ACATGGGGACCTGCTGGAGCTGGACCCCCCGGCCAACCACACCCCCTGTGTGGTACAGGT
GCACACACTCACTGGAGCCTTCCTCTTCTCCCTTGAATCCCAAACCACCATTGGCTATGG
CTTCCGCTACATCAGTGAGGAATGTCCACTGGCCATTGTGCTTCTTATTGCCCAGCTGGT
GCTCACCACCATCCTGGAAATCTTCATCACAGGTACCTTCCTGGCGAAGATTGCCCGGCC
CAAGAAGCGGGCTGAGACCATTCGTTTCAGCCAGCATGCAGTTGTGGCCTCCCACAATGG
CAAGCCCTGCCTCATGATCCGAGTTGCCAATATGCGCAAAAGCCTCCTCATTGGCTGCCA
GGTGACAGGAAAACTGCTTCAGACCCACCAAACCAAGGAAGGGGAGAACATCCGGCTCAA
CCAGGTCAATGTGACTTTCCAAGTAGACACAGCCTCTGACAGCCCCTTCCTTATTCTACC
CCTTACCTTCTATCATGTGGTAGATGAGACCAGTCCCTTGAAAGATCTCCCTCTTCGCAG
TGGTGAGGGTGACTTTGAGCTGGTGCTGATCCTAAGTGGGACAGTGGAGTCCACCAGTGC
CACCTGTCAGGTGCGCACTTCCTACCTGCCAGAGGAGATCCTTTGGGGCTACGAGTTCAC
ACCTGCCATCTCACTGTCAGCCAGTGGTAAATACATAGCTGACTTTAGCCTTTTTGACCA
AGTTGTGAAAGTGGCCTCTCCTAGTGGCCTCCGTGACAGCACTGTACGCTACGGAGACCC
TGAAAAGCTCAAGTTGGAGGAGTCATTAAGGGAGCAAGCTGAGAAGGAGGGCAGTGCCCT
TAGTGTGCGCATCAGCAATGTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT