Restriction map for
pFN21AB3644
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AccII AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI HincII
HindII HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 3 GACGTC GACGT^C
Acc65I 487 GGTACC G^GTACC
AccI 718 GTAGAC GT^[AC][GT]AC
AcyI 3 GACGTC G[GA]^CG[CT]C
Alw44I 334 GTGCAC G^TGCAC
AlwNI 725 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 140 AGCGCT AGC^GCT
ApaI 63 GGGCCC GGGCC^C
ApaLI 334 GTGCAC G^TGCAC
Asp718I 487 GGTACC G^GTACC
BalI 425 TGGCCA TGG^CCA
BanI 487 GGTACC G^G[CT][GA]CC
BanII 63 GGGCCC G[GA]GC[CT]^C
BciVI 71 GGATAC GTATCC(6/5)
BfaI 1016 CTAG C^TAG
BglII 798 AGATCT A^GATCT
BsaHI 3 GACGTC G[GA]^CG[CT]C
BspHI 587 TCATGA T^CATGA
BsrFI 50 GCCGGC [GA]^CCGG[CT]
BstAPI 575 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 461 CCACCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 50 GCCGGC [GA]^CCGG[CT]
DrdI 855 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 140 AGCGCT AGC^GCT
EcoNI 900 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 522 GCGGG CCCGC(4/6)
Hin1I 3 GACGTC G[GA]^CG[CT]C
HindIII 199 AAGCTT A^AGCTT
KpnI 487 GGTACC GGTAC^C
MaeI 1016 CTAG C^TAG
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MseI 1081 TTAA T^TAA
MslI 177 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 448 CAGCTG C[AC]G^C[GT]G
NaeI 50 GCCGGC GCC^GGC
NgoMIV 50 GCCGGC G^CCGGC
PvuII 448 CAGCTG CAG^CTG
RcaI 587 TCATGA T^CATGA
SapI 207 GCTCTTC GCTCTTC(1/4)
SexAI 695 ACCAGGT A^CC[AT]GGT
SfaNI 1124 GCATC GCATC(5/9)
SphI 549 GCATGC GCATG^C
TfiI 370 GAATC G^A[AT]TC
Tru9I 1081 TTAA T^TAA
XcmI 375 CCAAACCACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BbvI 107 GCAGC GCAGC(8/12)
629 GCTGC
BcnI 303 CCCGG CC^[GC]GG
509 CCCGG
BpmI 289 CTGGAG CTGGAG(16/14)
347 CTGGAG
BsrDI 129 CATTGC GCAATG(2/0)
1130 GCAATG
BstF5I 465 CATCC GGATG(2/0)
684 CATCC
BstYI 798 AGATCT [GA]^GATC[CT]
911 AGATCC
DraII 158 AGGACCT [GA]G^G[ACGT]CC[CT]
281 GGGACCT
EaeI 305 CGGCCA [CT]^GGCC[GA]
425 TGGCCA
EcoO109I 158 AGGACCT [GA]G^G[ACGT]CC[CT]
281 GGGACCT
EcoT14I 14 CCAAGG C^C[AT][AT]GG
668 CCAAGG
Fnu4HI 107 GCAGC GC^[ACGT]GC
629 GCTGC
FokI 465 CATCC GGATG(9/13)
684 CATCC
HaeII 140 AGCGCT [GA]GCGC^[CT]
191 GGCGCT
ItaI 107 GCAGC GC^[ACGT]GC
629 GCTGC
MaeII 4 ACGT A^CGT
113 ACGT
MflI 798 AGATCT [GA]^GATC[CT]
911 AGATCC
NciI 303 CCCGG CC^[GC]GG
509 CCCGG
NspI 182 ACATGC [GA]CATG^[CT]
549 GCATGC
PpuMI 158 AGGACCT [GA]G^G[AT]CC[CT]
281 GGGACCT
SmlI 154 CTCAAG C^T[CT][GA]AG
1063 CTCAAG
StyI 14 CCAAGG C^C[AT][AT]GG
668 CCAAGG
TseI 107 GCAGC G^C[AT]GC
629 GCTGC
XhoII 798 AGATCT [GA]^GATC[CT]
911 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII AviII BmyI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII FspI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI Ksp632I MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI Tsp45I TspRI