Restriction map for
pFN21AB3644
(Circular) (Six-base) MAPSORT of: pFN21AB3644.seq Check: 5610 from: 1 to: 5842
pFN21AB3644 5842 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2950 2950
Size: 5842
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2012 3652 278
Size: 53 83 186 936 476 1640 2468
Fragments arranged by size:
2468 1640 936 476 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2724 3185 1937
Size: 787 461 4594
Fragments arranged by size:
4594 787 461
Acc65I G'GTAC_C
Cuts at: 2492 3163 2492
Size: 671 5171
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 3905 5289 5557 1632
Size: 1010 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1010 268
AfeI AGC'GCT
Cuts at: 2147 2147
Size: 5842
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 2735 3585 5157 2735
Size: 850 1572 3420
Fragments arranged by size:
3420 1572 850
ApaI G_GGCC'C
Cuts at: 2072 2072
Size: 5842
ApaLI G'TGCA_C
Cuts at: 2339 3899 5055 2339
Size: 1560 1156 3126
Fragments arranged by size:
3126 1560 1156
ApoI r'AATT_y
Cuts at: 1493 3151 3377 3529 5638 1493
Size: 1658 226 152 2109 1697
Fragments arranged by size:
2109 1697 1658 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 3167 1870
Size: 73 1224 4545
Fragments arranged by size:
4545 1224 73
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2492 3163 4618 618
Size: 325 217 627 705 671 1455 1842
Fragments arranged by size:
1842 1455 705 671 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 3161 1391
Size: 681 1089 4072
Fragments arranged by size:
4072 1089 681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2070 3734 4944 2070
Size: 1664 1210 2968
Fragments arranged by size:
2968 1664 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4453 136
Size: 107 122 71 631 605 2781 1525
Fragments arranged by size:
2781 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2803 5837 2803
Size: 3034 2808
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2343 3118 3903 5059 1790
Size: 282 271 775 785 1156 2573
Fragments arranged by size:
2573 1156 785 775 282 271
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3573 4531 442
Size: 416 53 83 399 24 2156 958 1753
Fragments arranged by size:
2156 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2378 2378
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2373 4502 1239
Size: 1076 58 2129 2579
Fragments arranged by size:
2579 2129 1076 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 3053 3967 4832 5130 2144
Size: 909 914 865 298 2856
Fragments arranged by size:
2856 914 909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 2780 3048 4505 882
Size: 617 1029 252 268 1457 2219
Fragments arranged by size:
2219 1457 1029 617 268 252
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3649 4031 4666
Size: 53 83 186 936 476 1640 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1640 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4275 4673 4947 5094 5505 4275
Size: 398 274 147 411 4612
Fragments arranged by size:
4612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5617
Fragments arranged by size:
5617 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 2927 3092 1186
Size: 1429 312 165 3936
Fragments arranged by size:
3936 1429 312 165
BseYI C'CCAG_C
Cuts at: 2451 5045 5541 2451
Size: 2594 496 2752
Fragments arranged by size:
2752 2594 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2343 2463 3903 3988 5059 2133
Size: 210 120 1440 85 1071 2916
Fragments arranged by size:
2916 1440 1071 210 120 85
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2133 2343 2463 3118 3161 3903
Size: 399 282 61 210 120 655 43 742
Cuts at: 3903 3988 5059 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 655 399 282 210 120
85 61 43
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 2592 3731 5461 2592
Size: 1139 1730 2973
Fragments arranged by size:
2973 1730 1139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2913 2950 3197 844
Size: 1454 615 37 247 3489
Fragments arranged by size:
3489 1454 615 247 37
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2133 3142 4337 4511 65
Size: 1183 885 1009 1195 174 1396
Fragments arranged by size:
1396 1195 1183 1009 885 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 4486 4673 1700
Size: 110 245 2431 187 2869
Fragments arranged by size:
2869 2431 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2586 794
Size: 1792 4050
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2803 2916 3172 3539 3924 3941 5393 5479
Size: 831 113 256 367 385 17 1452 86
Cuts at: 5479 5491 5837 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 831 385 367 346 256 113
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 2198 2824 3118 3358 4152 4172 2198
Size: 626 294 240 794 20 3868
Fragments arranged by size:
3868 794 626 294 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2866 4849 809
Size: 507 1550 1983 1802
Fragments arranged by size:
1983 1802 1550 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2430 3289
Size: 54 1425 50 275 18 480 120 859
Cuts at: 3289 4178 5807 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 859 480 275 120 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 2369 2817 3772 1353
Size: 866 150 448 955 3423
Fragments arranged by size:
3423 955 866 448 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2101 4429 4803 4949 387
Size: 1064 138 512 2328 374 146 1280
Fragments arranged by size:
2328 1280 1064 512 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2373 2642 3905 4502 5289 5557
Size: 393 683 58 269 1263 597 787 268
Cuts at: 5557 1239
Size: 1524
Fragments arranged by size:
1524 1263 787 683 597 393 269 268
58
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 2909
Size: 5842
EcoO109I rG'GnC_Cy
Cuts at: 2164 2287 2164
Size: 123 5719
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FspI TGC'GCA
Cuts at: 2615 2894 3128 4348 2615
Size: 279 234 1220 4109
Fragments arranged by size:
4109 1220 279 234
FspAI rTGC'GCAy
Cuts at: 2894 3128 2894
Size: 234 5608
HaeII r_GCGC'y
Cuts at: 1678 2149 2200 4989 1678
Size: 471 51 2789 2531
Fragments arranged by size:
2789 2531 471 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2717 2749 4483 4515 4557
Size: 32 135 32 1145 32 1734 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 1734 1145 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2204 748
Size: 1456 4386
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 2496 3167 2496
Size: 671 5171
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3054 4956 5140 1305
Size: 1749 1902 184 2007
Fragments arranged by size:
2007 1902 1749 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 3420
Fragments arranged by size:
3420 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 3800 4159 4318 518
Size: 648 1020 1614 359 159 2042
Fragments arranged by size:
2042 1614 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2455 3258 3935 5083 5328 1606
Size: 849 803 677 1148 245 2120
Fragments arranged by size:
2120 1148 849 803 677 245
NaeI GCC'GGC
Cuts at: 2057 2057
Size: 5842
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5290
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NgoMIV G'CCGG_C
Cuts at: 2055 2055
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2191 2558 3200 4745 1132
Size: 97 962 367 642 1545 2229
Fragments arranged by size:
2229 1545 962 642 367 97
PasI CC'CwG_GG
Cuts at: 1348 2072 1348
Size: 724 5118
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5842
PfoI T'CCnGG_A
Cuts at: 2472 2472
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2287 2164
Size: 123 5719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PspOMI G'GGCC_C
Cuts at: 2068 2068
Size: 5842
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
PvuII CAG'CTG
Cuts at: 2455 2455
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SapI GCTCTTCn'nnn_
Cuts at: 2219 2219
Size: 5842
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 5842
SfcI C'TryA_G
Cuts at: 826 1045 3190 4325 5006 5197 826
Size: 219 2145 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 3068 3946 4847 5109 820
Size: 197 926 216 909 878 901 262 1553
Fragments arranged by size:
1553 926 909 901 878 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 2558 3200 1132
Size: 1426 642 3774
Fragments arranged by size:
3774 1426 642
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2019 2673 3281 513
Size: 552 954 654 608 3074
Fragments arranged by size:
3074 954 654 608 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3869 3886 4039 4224 4669 325
Size: 1255 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4088 5800 96
Size: 274 80 33 51 1443 2111 1712 138
Fragments arranged by size:
2111 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5802 5834 5802
Size: 32 5810
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2387 2387
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 3971 3971
Size: 5842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2010 3650 276
Size: 53 83 186 936 476 1640 2468
Fragments arranged by size:
2468 1640 936 476 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI