NELFB ORF clone pFN21AA1182

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AA1182

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Clone info.

Species

human

Clone Name

pFN21AA1182 Sequence (with vector) Restriction Map

Product ID

FHC00195

Flexi Clone PID

FXC00195

Original Clone PID

ORK00195

ORF Sequence

DNA sequence (1740 bp)
Protein sequence (580 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384577]

Gene

KIAA1182

Symbol

NELFB

Alias : COBRA1, NELF-B

Description

negative elongation factor complex member B

Original Clone ID

ha03446

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1740 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 580 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7040.2 0 100.0 NELFB The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR010405 106 579 PF06209 Cofactor of BRCA1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056271 628 611180 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1182 Download

>KIAA1182  1740 bp
ATGTTCGCGGGGCTGCAGGACCTGGGCGTGGCCAACGGCGAGGACCTGAAGGAGACCCTG
ACCAACTGCACGGAGCCGCTCAAGGCCATCGAGCAGTTCCAGACAGAGAATGGTGTGCTG
CTGCCATCTCTTCAGTCAGCCCTCCCCTTCTTGGACCTGCACGGGACGCCGCGGCTGGAG
TTCCACCAGTCGGTATTCGATGAGCTGCGGGACAAGCTGCTGGAGCGAGTGTCAGCCATC
GCTTCGGAGGGGAAGGCTGAGGAAAGGTACAAGAAGCTGGAAGACCTTCTGGAGAAGAGC
TTTTCTCTGGTGAAGATGCCGTCCCTGCAGCCCGTGGTGATGTGCGTCATGAAGCACCTG
CCCAAGGTTCCGGAGAAAAAACTGAAGCTGGTTATGGCTGACAAGGAGCTGTATCGAGCC
TGCGCCGTGGAGGTGAAGCGGCAGATCTGGCAAGACAACCAGGCCCTCTTCGGGGACGAG
GTTTCCCCACTCCTGAAGCAGTACATCCTGGAGAAGGAGAGCGCTCTCTTCAGTACAGAG
CTCTCTGTCCTGCACAACTTTTTCAGTCCTTCCCCCAAGACCAGGCGCCAGGGCGAGGTG
GTGCAGCGGCTGACGCGGATGGTGGGGAAGAACGTGAAGCTGTACGACATGGTGCTGCAG
TTTCTGCGCACGCTCTTCCTGCGCACGCGGAATGTGCACTACTGCACGCTGCGGGCTGAG
CTGCTCATGTCCCTGCACGACCTGGACGTGGGTGAAATCTGCACCGTGGACCCGTGCCAC
AAGTTCACCTGGTGCCTGGACGCCTGCATCCGAGAGCGGTTCGTGGACAGCAAGAGGGCG
CGGGAGCTGCAGGGGTTTCTCGATGGCGTCAAGAAGGGCCAGGAGCAGGTGCTGGGGGAC
CTGTCCATGATCCTGTGTGACCCCTTCGCCATCAACACGCTGGCACTGAGCACAGTCAGG
CACCTGCAGGAGCTGGTCGGCCAGGAGACACTGCCCAGGGACAGCCCCGACCTCCTGCTG
CTGCTCCGGCTGCTGGCGCTGGGCCAGGGAGCCTGGGACATGATCGACAGCCAGGTCTTC
AAGGAGCCCAAGATGGAGGTAGAGCTCATCACCAGGTTCCTCCCGATGCTCATGTCCTTC
CTGGTGGATGACTACACTTTCAATGTGGATCAGAAACTTCCGGCTGAGGAGAAAGCCCCA
GTCTCATATCCAAACACACTTCCCGAAAGCTTCACTAAGTTTCTGCAGGAGCAGCGCATG
GCCTGCGAGGTGGGGCTGTACTACGTCCTGCACATCACCAAGCAGAGGAACAAGAACGCG
CTCCTCCGCCTGCTGCCCGGGCTGGTGGAGACCTTTGGCGACTTGGCCTTTGGCGACATC
TTCCTCCACCTGCTCACGGGCAACCTTGCGCTGCTGGCCGACGAATTTGCCCTTGAGGAC
TTCTGCAGCAGCCTCTTCGATGGCTTCTTCCTCACCGCCTCTCCAAGGAAGGAGAACGTG
CACCGGCACGCGCTGCGGCTCCTCATTCACCTGCACCCCAGGGTGGCCCCATCTAAGCTG
GAGGCGTTGCAGAAGGCCCTGGAGCCTACAGGCCAGAGCGGAGAGGCAGTGAAGGAGCTT
TACTCCCAGCTCGGCGAGAAGCTGGAACAGCTGGATCACCGGAAGCCCAGCCCGGCACAG
GCTGCGGAGACGCCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCCGCTC

ORF Protein

Cloned ORF protein sequence for pFN21AA1182 Download

>KIAA1182  580 aa
MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLE
FHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHL
PKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTE
LSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAE
LLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGD
LSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVF
KEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRM
ACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALED
FCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKEL
YSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1182 Download

>pFN21AA1182  6445 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCGCGGGGCTGCAGGACCTGGGCGTGGCCAA
CGGCGAGGACCTGAAGGAGACCCTGACCAACTGCACGGAGCCGCTCAAGGCCATCGAGCA
GTTCCAGACAGAGAATGGTGTGCTGCTGCCATCTCTTCAGTCAGCCCTCCCCTTCTTGGA
CCTGCACGGGACGCCGCGGCTGGAGTTCCACCAGTCGGTATTCGATGAGCTGCGGGACAA
GCTGCTGGAGCGAGTGTCAGCCATCGCTTCGGAGGGGAAGGCTGAGGAAAGGTACAAGAA
GCTGGAAGACCTTCTGGAGAAGAGCTTTTCTCTGGTGAAGATGCCGTCCCTGCAGCCCGT
GGTGATGTGCGTCATGAAGCACCTGCCCAAGGTTCCGGAGAAAAAACTGAAGCTGGTTAT
GGCTGACAAGGAGCTGTATCGAGCCTGCGCCGTGGAGGTGAAGCGGCAGATCTGGCAAGA
CAACCAGGCCCTCTTCGGGGACGAGGTTTCCCCACTCCTGAAGCAGTACATCCTGGAGAA
GGAGAGCGCTCTCTTCAGTACAGAGCTCTCTGTCCTGCACAACTTTTTCAGTCCTTCCCC
CAAGACCAGGCGCCAGGGCGAGGTGGTGCAGCGGCTGACGCGGATGGTGGGGAAGAACGT
GAAGCTGTACGACATGGTGCTGCAGTTTCTGCGCACGCTCTTCCTGCGCACGCGGAATGT
GCACTACTGCACGCTGCGGGCTGAGCTGCTCATGTCCCTGCACGACCTGGACGTGGGTGA
AATCTGCACCGTGGACCCGTGCCACAAGTTCACCTGGTGCCTGGACGCCTGCATCCGAGA
GCGGTTCGTGGACAGCAAGAGGGCGCGGGAGCTGCAGGGGTTTCTCGATGGCGTCAAGAA
GGGCCAGGAGCAGGTGCTGGGGGACCTGTCCATGATCCTGTGTGACCCCTTCGCCATCAA
CACGCTGGCACTGAGCACAGTCAGGCACCTGCAGGAGCTGGTCGGCCAGGAGACACTGCC
CAGGGACAGCCCCGACCTCCTGCTGCTGCTCCGGCTGCTGGCGCTGGGCCAGGGAGCCTG
GGACATGATCGACAGCCAGGTCTTCAAGGAGCCCAAGATGGAGGTAGAGCTCATCACCAG
GTTCCTCCCGATGCTCATGTCCTTCCTGGTGGATGACTACACTTTCAATGTGGATCAGAA
ACTTCCGGCTGAGGAGAAAGCCCCAGTCTCATATCCAAACACACTTCCCGAAAGCTTCAC
TAAGTTTCTGCAGGAGCAGCGCATGGCCTGCGAGGTGGGGCTGTACTACGTCCTGCACAT
CACCAAGCAGAGGAACAAGAACGCGCTCCTCCGCCTGCTGCCCGGGCTGGTGGAGACCTT
TGGCGACTTGGCCTTTGGCGACATCTTCCTCCACCTGCTCACGGGCAACCTTGCGCTGCT
GGCCGACGAATTTGCCCTTGAGGACTTCTGCAGCAGCCTCTTCGATGGCTTCTTCCTCAC
CGCCTCTCCAAGGAAGGAGAACGTGCACCGGCACGCGCTGCGGCTCCTCATTCACCTGCA
CCCCAGGGTGGCCCCATCTAAGCTGGAGGCGTTGCAGAAGGCCCTGGAGCCTACAGGCCA
GAGCGGAGAGGCAGTGAAGGAGCTTTACTCCCAGCTCGGCGAGAAGCTGGAACAGCTGGA
TCACCGGAAGCCCAGCCCGGCACAGGCTGCGGAGACGCCGGCCCTGGAGCTGCCCCTCCC
CAGCGTGCCCGCCCCTGCCCCGCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT