Restriction map for
pFN21AA1182
(Linear) (Six-base) MAPSORT of: KIAA1182.seq Check: 1822 from: 1 to: 1740
KIAA1182.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 365 876 971 1397 1538 1740
Size: 365 511 95 426 141 202
Fragments arranged by size:
511 426 365 202 141 95
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 115 403 513 514 1740
Size: 67 48 288 110 1 1226
Fragments arranged by size:
1226 288 110 67 48 1
AfeI AGC'GCT
Cuts at: 0 522 1740
Size: 522 1218
AlwNI CAG_nnn'CTG
Cuts at: 0 21 609 663 891 972 1740
Size: 21 588 54 228 81 768
Fragments arranged by size:
768 588 228 81 54 21
ApaLI G'TGCA_C
Cuts at: 0 694 1498 1740
Size: 694 804 242
Fragments arranged by size:
804 694 242
ApoI r'AATT_y
Cuts at: 0 1423 1740
Size: 1423 317
AvaI C'yCGr_G
Cuts at: 0 1336 1740
Size: 1336 404
BanI G'GyrC_C
Cuts at: 0 584 791 959 1740
Size: 584 207 168 781
Fragments arranged by size:
781 584 207 168
BanII G_rGCy'C
Cuts at: 0 542 1088 1106 1740
Size: 542 546 18 634
Fragments arranged by size:
634 546 542 18
BbeI G_GCGC'C
Cuts at: 0 588 1740
Size: 588 1152
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 1068 1740
Size: 287 781 672
Fragments arranged by size:
781 672 287
BbvCI CC'TCA_GC
Cuts at: 0 257 1184 1740
Size: 257 927 556
Fragments arranged by size:
927 556 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1408 1437 1442 1471 1740
Size: 1408 29 5 29 269
Fragments arranged by size:
1408 269 29 29 5
BglII A'GATC_T
Cuts at: 0 443 1740
Size: 443 1297
BlpI GC'TnA_GC
Cuts at: 0 716 1740
Size: 716 1024
Bme1580I G_kGCm'C
Cuts at: 0 698 1502 1724 1740
Size: 698 804 222 16
Fragments arranged by size:
804 698 222 16
BmgBI CAC'GTC
Cuts at: 0 747 1740
Size: 747 993
BmrI ACTGGGnnnn_n'
Cuts at: 0 1192 1740
Size: 1192 548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 320 1638 1740
Size: 320 1318 102
Fragments arranged by size:
1318 320 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 241 310 529 1579 1600 1720 1740
Size: 196 45 69 219 1050 21 120 20
Fragments arranged by size:
1050 219 196 120 69 45 21 20
Bpu10I CC'TnA_GC
Cuts at: 0 257 1184 1740
Size: 257 927 556
Fragments arranged by size:
927 556 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1453 1740
Size: 64 1389 287
Fragments arranged by size:
1389 287 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 1342 1740
Size: 46 1296 398
Fragments arranged by size:
1296 398 46
BsaHI Gr'CG_yC
Cuts at: 0 166 585 800 866 1690 1740
Size: 166 419 215 66 824 50
Fragments arranged by size:
824 419 215 166 66 50
BsaWI w'CCGG_w
Cuts at: 0 369 1658 1740
Size: 369 1289 82
Fragments arranged by size:
1289 369 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 1464 1494 1592 1622 1740
Size: 214 30 1220 30 98 30 118
Fragments arranged by size:
1220 214 118 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1201 1312 1510 1740
Size: 1201 111 198 230
Fragments arranged by size:
1201 230 198 111
BseYI C'CCAG_C
Cuts at: 0 891 1038 1625 1666 1714 1740
Size: 891 147 587 41 48 26
Fragments arranged by size:
891 587 147 48 41 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 142 535 622 687 718 744 1273
Size: 51 91 393 87 65 31 26 529
Cuts at: 1273 1516 1740
Size: 243 224
Fragments arranged by size:
529 393 243 224 91 87 65 51
31 26
BsiHKAI G_wGCw'C
Cuts at: 0 542 698 952 1106 1502 1740
Size: 542 156 254 154 396 238
Fragments arranged by size:
542 396 254 238 156 154
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1681 1740
Size: 1681 59
Bsp1286I G_dGCh'C
Cuts at: 0 542 698 952 1088 1106 1502 1724 1740
Size: 542 156 254 136 18 396 222 16
Fragments arranged by size:
542 396 254 222 156 136 18 16
BspEI T'CCGG_A
Cuts at: 0 369 1740
Size: 369 1371
BspHI T'CATG_A
Cuts at: 0 347 1740
Size: 347 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 365 876 971 1397 1538 1740
Size: 164 201 511 95 426 141 202
Fragments arranged by size:
511 426 202 201 164 141 95
BsrBI CCG'CTC
Cuts at: 0 78 816 1598 1737 1740
Size: 78 738 782 139 3
Fragments arranged by size:
782 738 139 78 3
BsrFI r'CCGG_y
Cuts at: 0 1502 1692 1740
Size: 1502 190 48
Fragments arranged by size:
1502 190 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 663 1262 1512 1681 1740
Size: 663 599 250 169 59
Fragments arranged by size:
663 599 250 169 59
BstYI r'GATC_y
Cuts at: 0 443 1740
Size: 443 1297
BtgI C'CryG_G
Cuts at: 0 169 332 425 764 1740
Size: 169 163 93 339 976
Fragments arranged by size:
976 339 169 163 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 222 1740
Size: 222 1518
BtsI GCAGTG_nn'
Cuts at: 0 988 1613 1740
Size: 988 625 127
Fragments arranged by size:
988 625 127
DraIII CAC_nnn'GTG
Cuts at: 0 791 1740
Size: 791 949
DrdI GACnn_nn'nnGTC
Cuts at: 0 1072 1740
Size: 1072 668
EaeI y'GGCC_r
Cuts at: 0 29 978 1415 1740
Size: 29 949 437 325
Fragments arranged by size:
949 437 325 29
EarI CTCTTCn'nnn_
Cuts at: 0 134 289 472 532 679 1459 1740
Size: 134 155 183 60 147 780 281
Fragments arranged by size:
780 281 183 155 147 134 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1315 1740
Size: 1315 425
EcoICRI GAG'CTC
Cuts at: 0 540 1104 1740
Size: 540 564 636
Fragments arranged by size:
636 564 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 115 196 241 310 403 513 514
Size: 67 48 81 45 69 93 110 1
Cuts at: 514 529 1579 1600 1720 1740
Size: 15 1050 21 120 20
Fragments arranged by size:
1050 120 110 93 81 69 67 48
45 21 20 15 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 511 1482 1740
Size: 361 150 971 258
Fragments arranged by size:
971 361 258 150
EcoO109I rG'GnC_Cy
Cuts at: 0 18 42 462 897 1575 1740
Size: 18 24 420 435 678 165
Fragments arranged by size:
678 435 420 165 24 18
FspI TGC'GCA
Cuts at: 0 667 682 1740
Size: 667 15 1058
Fragments arranged by size:
1058 667 15
HaeII r_GCGC'y
Cuts at: 0 524 588 1039 1740
Size: 524 64 451 701
Fragments arranged by size:
701 524 451 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 1740
Size: 214 32 1494
Fragments arranged by size:
1494 214 32
HindIII A'AGCT_T
Cuts at: 0 1227 1740
Size: 1227 513
KasI G'GCGC_C
Cuts at: 0 584 1740
Size: 584 1156
MscI TGG'CCA
Cuts at: 0 31 1740
Size: 31 1709
MspA1I CmG'CkG
Cuts at: 0 171 606 1650 1740
Size: 171 435 1044 90
Fragments arranged by size:
1044 435 171 90
NaeI GCC'GGC
Cuts at: 0 1694 1740
Size: 1694 46
NarI GG'CG_CC
Cuts at: 0 585 1740
Size: 585 1155
NgoMIV G'CCGG_C
Cuts at: 0 1692 1740
Size: 1692 48
PasI CC'CwG_GG
Cuts at: 0 995 1538 1740
Size: 995 543 202
Fragments arranged by size:
995 543 202
PfoI T'CCnGG_A
Cuts at: 0 506 1740
Size: 506 1234
PpuMI rG'GwC_Cy
Cuts at: 0 18 42 897 1740
Size: 18 24 855 843
Fragments arranged by size:
855 843 24 18
PstI C_TGCA'G
Cuts at: 0 17 329 659 851 968 1247 1447 1740
Size: 17 312 330 192 117 279 200 293
Fragments arranged by size:
330 312 293 279 200 192 117 17
PvuII CAG'CTG
Cuts at: 0 1650 1740
Size: 1650 90
SacI G_AGCT'C
Cuts at: 0 542 1106 1740
Size: 542 564 634
Fragments arranged by size:
634 564 542
SacII CC_GC'GG
Cuts at: 0 172 1740
Size: 172 1568
SapI GCTCTTCn'nnn_
Cuts at: 0 289 679 1740
Size: 289 390 1061
Fragments arranged by size:
1061 390 289
SbfI CC_TGCA'GG
Cuts at: 0 968 1740
Size: 968 772
SexAI A'CCwGG_T
Cuts at: 0 787 1111 1740
Size: 787 324 629
Fragments arranged by size:
787 629 324
SfcI C'TryA_G
Cuts at: 0 13 325 655 847 964 1243 1443 1586
Size: 13 312 330 192 117 279 200 143
Cuts at: 1586 1740
Size: 154
Fragments arranged by size:
330 312 279 200 192 154 143 117
13
SfoI GGC'GCC
Cuts at: 0 586 1740
Size: 586 1154
SmaI CCC'GGG
Cuts at: 0 1338 1740
Size: 1338 402
SmlI C'TyrA_G
Cuts at: 0 79 1432 1740
Size: 79 1353 308
Fragments arranged by size:
1353 308 79
SrfI GCCC'GGGC
Cuts at: 0 1338 1740
Size: 1338 402
StyI C'CwwG_G
Cuts at: 0 362 1483 1740
Size: 362 1121 257
Fragments arranged by size:
1121 362 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 739 1740
Size: 739 1001
TatI w'GTAC_w
Cuts at: 0 500 532 1277 1740
Size: 500 32 745 463
Fragments arranged by size:
745 500 463 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1464 1496 1592 1624 1740
Size: 1464 32 96 32 116
Fragments arranged by size:
1464 116 96 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 936 1740
Size: 106 830 804
Fragments arranged by size:
830 804 106
XmaI C'CCGG_G
Cuts at: 0 1336 1740
Size: 1336 404
XmnI GAAnn'nnTTC
Cuts at: 0 284 1740
Size: 284 1456
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BstAPI BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII KasI
MscI MspA1I NaeI NarI NgoMIV PasI PfoI PpuMI
PstI PvuII SacI SacII SapI SbfI SexAI SfcI
SfoI SmaI SmlI SrfI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglI BmtI BsaAI
BsaBI BsiEI BsiWI BsmI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
EagI EcoRI EcoRV FalI FseI FspAI HincII HpaI
KpnI MfeI MluI MmeI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI Tth111I XbaI XhoI
ZraI