Restriction map for
pFN21AA1182
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbrPI BciVI
BclI BfaI BfrI BglI BlnI
BsaAI BsaBI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HinfI HpaI KpnI MaeI MamI
MfeI MluI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 369 TCCGGA T^CCGGA
AcsI 1423 GAATTT [GA]^AATT[CT]
Aor51HI 520 AGCGCT AGC^GCT
ApoI 1423 GAATTT [GA]^AATT[CT]
Asp700I 280 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1336 CCCGGG C^[CT]CG[GA]G
BalI 29 TGGCCA TGG^CCA
BbeI 584 GGCGCC GGCGC^C
BglII 443 AGATCT A^GATCT
BlpI 715 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 715 GCTGAGC GC^T[ACGT]AGC
BseAI 369 TCCGGA T^CCGGA
BsmBI 1687 GAGACG CGTCTC(1/5)
BsoBI 1336 CCCGGG C^[CT]CG[GA]G
BspEI 369 TCCGGA T^CCGGA
BspHI 347 TCATGA T^CATGA
BstYI 443 AGATCT [GA]^GATC[CT]
CelII 715 GCTGAGC GC^T[ACGT]AGC
DraIII 786 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1066 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 520 AGCGCT AGC^GCT
HindIII 1227 AAGCTT A^AGCTT
KasI 584 GGCGCC G^GCGCC
KspI 169 CCGCGG CCGC^GG
MaeIII 917 GTGAC ^GT[ACGT]AC
MflI 443 AGATCT [GA]^GATC[CT]
MluNI 29 TGGCCA TGG^CCA
MroI 369 TCCGGA T^CCGGA
MscI 29 TGGCCA TGG^CCA
NaeI 1692 GCCGGC GCC^GGC
NarI 584 GGCGCC GG^CGCC
NgoMIV 1692 GCCGGC G^CCGGC
PvuII 1648 CAGCTG CAG^CTG
RcaI 347 TCATGA T^CATGA
SacII 169 CCGCGG CCGC^GG
SbfI 963 CCTGCAGG CCTGCA^GG
SfoI 584 GGCGCC GGC^GCC
SmaI 1336 CCCGGG CCC^GGG
Sse8387I 963 CCTGCAGG CCTGCA^GG
Tsp45I 917 GTGAC ^GT[GC]AC
Tsp509I 1424 AATT ^AATT
XhoII 443 AGATCT [GA]^GATC[CT]
XmaCI 1336 CCCGGG C^CCGGG
XmaI 1336 CCCGGG C^CCGGG
XmnI 280 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 694 GTGCAC G^TGCAC
1498 GTGCAC
ApaLI 694 GTGCAC G^TGCAC
1498 GTGCAC
AviII 665 TGCGCA TGC^GCA
680 TGCGCA
BbsI 280 GAAGAC GAAGAC(2/6)
1075 GTCTTC
BbvCI 256 GCTGAGG CCTCAGC(-5/-2)
1183 GCTGAGG
BcgI 1419 CGACGAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1448 GCAGCCTCTTCG
BpuAI 280 GAAGAC GAAGAC(2/6)
1075 GTCTTC
BsaI 52 GAGACC GGTCTC(1/5)
1348 GAGACC
BsaWI 369 TCCGGA [AT]^CCGG[AT]
1658 ACCGGA
BsrFI 1502 ACCGGC [GA]^CCGG[CT]
1692 GCCGGC
BsrI 186 CCAGT ACTGG(1/-1)
1198 CCAGT
Cfr10I 1502 ACCGGC [GA]^CCGG[CT]
1692 GCCGGC
EcoT14I 362 CCAAGG C^C[AT][AT]GG
1483 CCAAGG
FspI 665 TGCGCA TGC^GCA
680 TGCGCA
SacI 538 GAGCTC GAGCT^C
1102 GAGCTC
SapI 294 GAAGAGC GCTCTTC(1/4)
672 GCTCTTC
SexAI 787 ACCTGGT A^CC[AT]GGT
1111 ACCAGGT
SmlI 79 CTCAAG C^T[CT][GA]AG
1432 CTTGAG
StyI 362 CCAAGG C^C[AT][AT]GG
1483 CCAAGG
XcmI 99 CCAGACAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
929 CCATCAACACGCTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I