(Circular) (Six-base) MAPSORT of: pFN21AA1182.seq Check: 496 from: 1 to: 6445 pFN21AA1182 6445 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2370 2881 2976 3402 3543 2370 Size: 511 95 426 141 5272 Fragments arranged by size: 5272 511 426 141 95 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 3766 3766 Size: 6445 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2120 2408 2518 2519 4508 5892 6160 Size: 440 48 288 110 1 1989 1384 268 Cuts at: 6160 1632 Size: 1917 Fragments arranged by size: 1989 1917 1384 440 288 268 110 48 1 AfeI AGC'GCT Cuts at: 2527 2527 Size: 6445 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AlwNI CAG_nnn'CTG Cuts at: 2026 2614 2668 2896 2977 4188 5760 2026 Size: 588 54 228 81 1211 1572 2711 Fragments arranged by size: 2711 1572 1211 588 228 81 54 ApaLI G'TGCA_C Cuts at: 2699 3503 4502 5658 2699 Size: 804 999 1156 3486 Fragments arranged by size: 3486 1156 999 804 ApoI r'AATT_y Cuts at: 1493 3428 3754 3980 4132 6241 1493 Size: 1935 326 226 152 2109 1697 Fragments arranged by size: 2109 1935 1697 326 226 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 3341 3770 1870 Size: 73 1398 429 4545 Fragments arranged by size: 4545 1398 429 73 BamHI G'GATC_C Cuts at: 3775 4142 3775 Size: 367 6078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2589 2796 2964 3766 5221 Size: 325 217 627 802 207 168 802 1455 Cuts at: 5221 618 Size: 1842 Fragments arranged by size: 1842 1455 802 802 627 325 217 207 168 BanII G_rGCy'C Cuts at: 1391 2547 3093 3111 3764 1391 Size: 1156 546 18 653 4072 Fragments arranged by size: 4072 1156 653 546 18 BbeI G_GCGC'C Cuts at: 2593 2593 Size: 6445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 3073 928 Size: 971 393 781 4300 Fragments arranged by size: 4300 971 781 393 BbvCI CC'TCA_GC Cuts at: 2262 3189 2262 Size: 927 5518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3413 3442 3447 3476 4636 4670 1903 Size: 34 1476 29 5 29 1160 34 3678 Fragments arranged by size: 3678 1476 1160 34 34 29 29 5 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5056 136 Size: 107 122 71 631 605 3384 1525 Fragments arranged by size: 3384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2448 6440 2448 Size: 3992 2453 BlpI GC'TnA_GC Cuts at: 2721 3862 2721 Size: 1141 5304 Bme1580I G_kGCm'C Cuts at: 1790 2703 3507 3729 4506 5662 1790 Size: 913 804 222 777 1156 2573 Fragments arranged by size: 2573 1156 913 804 777 222 BmgBI CAC'GTC Cuts at: 1318 2752 1318 Size: 1434 5011 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3197 4176 5134 Size: 416 53 83 399 24 1780 979 958 Cuts at: 5134 442 Size: 1753 Fragments arranged by size: 1780 1753 979 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2325 3643 2325 Size: 1318 5127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2246 2315 2534 3584 3605 3725 5105 Size: 962 45 69 219 1050 21 120 1380 Cuts at: 5105 1239 Size: 2579 Fragments arranged by size: 2579 1380 1050 962 219 120 69 45 21 Bpu10I CC'TnA_GC Cuts at: 2262 3189 2262 Size: 927 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2069 3458 4570 5435 5733 2069 Size: 1389 1112 865 298 2781 Fragments arranged by size: 2781 1389 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 3347 5108 882 Size: 617 552 1296 1761 2219 Fragments arranged by size: 2219 1761 1296 617 552 BsaAI yAC'GTr Cuts at: 493 1204 4236 493 Size: 711 3032 2702 Fragments arranged by size: 3032 2702 711 BsaBI GATnn'nnATC Cuts at: 6439 6439 Size: 6445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2171 2590 2805 2871 Size: 53 83 186 936 638 419 215 66 Cuts at: 2871 3695 4252 4634 5269 275 Size: 824 557 382 635 1451 Fragments arranged by size: 1451 936 824 638 635 557 419 382 215 186 83 66 53 BsaWI w'CCGG_w Cuts at: 2374 3663 4878 5276 5550 5697 6108 2374 Size: 1289 1215 398 274 147 411 2711 Fragments arranged by size: 2711 1289 1215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 3469 3499 3597 Size: 30 165 30 816 30 1220 30 98 Cuts at: 3597 3627 1178 Size: 30 3996 Fragments arranged by size: 3996 1220 816 165 98 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3206 3317 3515 1186 Size: 2020 111 198 4116 Fragments arranged by size: 4116 2020 198 111 BseYI C'CCAG_C Cuts at: 2896 3043 3630 3671 3719 5648 6144 2896 Size: 147 587 41 48 1929 496 3197 Fragments arranged by size: 3197 1929 587 496 147 48 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2056 2147 2540 2627 2692 2723 2749 Size: 573 320 91 393 87 65 31 26 Cuts at: 2749 3278 3521 1163 Size: 529 243 4087 Fragments arranged by size: 4087 573 529 393 320 243 91 87 65 31 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2547 2703 2957 3111 3507 4506 4591 5662 2547 Size: 156 254 154 396 999 85 1071 3330 Fragments arranged by size: 3330 1071 999 396 254 156 154 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 BsmBI CGTCTCn'nnnn_ Cuts at: 3686 3686 Size: 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2547 2703 2957 3093 3111 3507 3729 Size: 399 757 156 254 136 18 396 222 Cuts at: 3729 3764 4506 4591 5662 1391 Size: 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 757 742 399 396 254 222 156 136 85 35 18 BspEI T'CCGG_A Cuts at: 2374 6108 2374 Size: 3734 2711 BspHI T'CATG_A Cuts at: 2352 4334 6064 2352 Size: 1982 1730 2733 Fragments arranged by size: 2733 1982 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 2370 2881 2976 3402 3543 3800 844 Size: 1325 201 511 95 426 141 257 3489 Fragments arranged by size: 3489 1325 511 426 257 201 141 95 BsrBI CCG'CTC Cuts at: 1394 1823 2083 2821 3603 3742 4332 1394 Size: 429 260 738 782 139 590 3507 Fragments arranged by size: 3507 782 738 590 429 260 139 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3507 3697 5089 5276 1700 Size: 110 1697 190 1392 187 2869 Fragments arranged by size: 2869 1697 1392 190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 4505 5517 4505 Size: 1012 5433 BstAPI GCAn_nnn'nTGC Cuts at: 794 2668 3267 3517 3686 794 Size: 1874 599 250 169 3553 Fragments arranged by size: 3553 1874 599 250 169 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6445 BstYI r'GATC_y Cuts at: 1972 2448 3775 4142 4527 4544 5996 6082 6094 Size: 476 1327 367 385 17 1452 86 12 Cuts at: 6094 6440 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1327 476 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2174 2337 2430 2769 513 Size: 552 1109 163 93 339 4189 Fragments arranged by size: 4189 1109 552 339 163 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2227 487 Size: 683 56 1001 4705 Fragments arranged by size: 4705 1001 683 56 BtsI GCAGTG_nn' Cuts at: 2993 3618 3961 4755 4775 2993 Size: 625 343 794 20 4663 Fragments arranged by size: 4663 794 625 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DraIII CAC_nnn'GTG Cuts at: 2796 2796 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3077 5452 809 Size: 507 1761 2375 1802 Fragments arranged by size: 2375 1802 1761 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2983 3420 Size: 54 1425 50 275 18 204 949 437 Cuts at: 3420 3892 4781 6410 8 Size: 472 889 1629 43 Fragments arranged by size: 1629 1425 949 889 472 437 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2294 2477 2537 2684 3464 4375 1353 Size: 786 155 183 60 147 780 911 3423 Fragments arranged by size: 3423 911 786 780 183 155 147 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3320 5032 5406 5552 387 Size: 1064 138 1731 1712 374 146 1280 Fragments arranged by size: 1731 1712 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2545 3109 2545 Size: 564 5881 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2120 2201 2246 2315 2408 2518 Size: 393 440 48 81 45 69 93 110 Cuts at: 2518 2519 2534 3584 3605 3725 4508 5105 5892 Size: 1 15 1050 21 120 783 597 787 Cuts at: 5892 6160 1239 Size: 268 1524 Fragments arranged by size: 1524 1050 787 783 597 440 393 268 120 110 93 81 69 48 45 21 15 1 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2516 3487 2366 Size: 150 971 5324 Fragments arranged by size: 5324 971 150 EcoO109I rG'GnC_Cy Cuts at: 2023 2047 2467 2902 3580 2023 Size: 24 420 435 678 4888 Fragments arranged by size: 4888 678 435 420 24 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 FspI TGC'GCA Cuts at: 2672 2687 4951 2672 Size: 15 2264 4166 Fragments arranged by size: 4166 2264 15 HaeII r_GCGC'y Cuts at: 1678 2529 2593 3044 5592 1678 Size: 851 64 451 2548 2531 Fragments arranged by size: 2548 2531 851 451 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 5086 5118 5160 Size: 32 135 32 647 32 2835 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2835 2626 647 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 3232 748 Size: 2484 3961 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KasI G'GCGC_C Cuts at: 2589 2589 Size: 6445 KpnI G_GTAC'C Cuts at: 3770 3770 Size: 6445 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5559 5743 1305 Size: 4254 184 2007 Fragments arranged by size: 4254 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 10 Size: 54 1425 343 204 4419 Fragments arranged by size: 4419 1425 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 4403 4762 4921 518 Size: 648 3237 359 159 2042 Fragments arranged by size: 3237 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2611 3655 3861 4538 5686 5931 1606 Size: 570 435 1044 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 1044 677 570 435 245 206 NaeI GCC'GGC Cuts at: 3699 3699 Size: 6445 NarI GG'CG_CC Cuts at: 2590 2590 Size: 6445 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5893 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NgoMIV G'CCGG_C Cuts at: 3697 3697 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 3803 5348 1132 Size: 97 2574 1545 2229 Fragments arranged by size: 2574 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3000 3543 1348 Size: 1652 543 4250 Fragments arranged by size: 4250 1652 543 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6445 PfoI T'CCnGG_A Cuts at: 2511 2511 Size: 6445 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2023 2047 2902 2023 Size: 24 855 5566 Fragments arranged by size: 5566 855 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PstI C_TGCA'G Cuts at: 830 2022 2334 2664 2856 2973 3252 3452 3797 Size: 1192 312 330 192 117 279 200 345 Cuts at: 3797 830 Size: 3478 Fragments arranged by size: 3478 1192 345 330 312 279 200 192 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 3655 3655 Size: 6445 SacI G_AGCT'C Cuts at: 2547 3111 2547 Size: 564 5881 SacII CC_GC'GG Cuts at: 2177 2177 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SapI GCTCTTCn'nnn_ Cuts at: 2294 2684 2294 Size: 390 6055 SbfI CC_TGCA'GG Cuts at: 2973 3797 2973 Size: 824 5621 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SexAI A'CCwGG_T Cuts at: 2792 3116 2792 Size: 324 6121 SfcI C'TryA_G Cuts at: 826 1045 2018 2330 2660 2852 2969 3248 3448 Size: 219 973 312 330 192 117 279 200 Cuts at: 3448 3591 3793 4928 5609 5800 826 Size: 143 202 1135 681 191 1471 Fragments arranged by size: 1471 1135 973 681 330 312 279 219 202 200 192 191 143 117 SfoI GGC'GCC Cuts at: 2591 2591 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3343 3772 1872 Size: 1471 429 4545 Fragments arranged by size: 4545 1471 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 3437 4549 5450 5712 820 Size: 197 926 141 1353 1112 901 262 1553 Fragments arranged by size: 1553 1353 1112 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SrfI GCCC'GGGC Cuts at: 3343 3343 Size: 6445 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6445 StyI C'CwwG_G Cuts at: 513 1065 2367 3488 3884 513 Size: 552 1302 1121 396 3074 Fragments arranged by size: 3074 1302 1121 552 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2744 4472 4489 4642 4827 5272 325 Size: 1255 1164 1728 17 153 185 445 1498 Fragments arranged by size: 1728 1498 1255 1164 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 2537 3282 Size: 274 80 33 51 1443 528 32 745 Cuts at: 3282 4691 6403 96 Size: 1409 1712 138 Fragments arranged by size: 1712 1443 1409 745 528 274 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3469 3501 3597 3629 6405 6437 3469 Size: 32 96 32 2776 32 3477 Fragments arranged by size: 3477 2776 96 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6445 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 2941 2111 Size: 830 5615 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 3341 3770 1870 Size: 1471 429 4545 Fragments arranged by size: 4545 1471 429 XmnI GAAnn'nnTTC Cuts at: 2289 4574 2289 Size: 2285 4160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SwaI