PPME1 ORF clone FHC01924

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01924

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Clone info.

Species

human

Clone Name

pFN21AB9511 Sequence (with vector) Restriction Map

Product ID

FHC01924

Flexi Clone PID

FXC01924

ORF Sequence

DNA sequence (1158 bp)
Protein sequence (386 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385584]

Gene

KIBB9511

Symbol

PPME1

Alias : ABDH19, PME-1

Description

protein phosphatase methylesterase 1, transcript variant 1

Original Clone ID

cp00146

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1158 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 386 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44678.1 8.2e-153 100.0 PPME1 CCDS60891.1 1.7e-105 96.5 PPME1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000073 105 167 PF00561 Alpha/beta hydrolase fold-1 ScanRegExp IPR008262 150 159 PS00120 Lipase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057231 386 611117 100.0 100.0 NP_001258522 400 611117 96.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9511 Download

>KIBB9511  1158 bp
ATGTCGGCCCTCGAAAAGAGCATGCACCTCGGCCGCCTTCCCTCTCGCCCACCTCTACCC
GGCAGCGGGGGCAGTCAGAGCGGAGCCAAGATGCGAATGGGCCCTGGAAGAAAGCGGGAC
TTTTCCCCTGTTCCTTGGAGTCAGTATTTTGAGTCCATGGAAGATGTAGAAGTAGAGAAT
GAAACTGGCAAGGATACTTTTCGAGTCTACAAGAGTGGTTCAGAGGGTCCAGTCCTGCTC
CTTCTGCATGGAGGAGGTCATTCTGCCCTTTCTTGGGCTGTGTTCACGGCAGCGATTATT
AGTAGAGTTCAGTGTAGGATTGTAGCTTTGGATCTGCGAAGTCATGGTGAAACAAAGGTC
AAGAATCCTGAAGATCTGTCTGCAGAAACAATGGCAAAAGACGTTGGCAATGTGGTTGAA
GCCATGTATGGGGACCTTCCTCCTCCAATTATGCTGATTGGACATAGCATGGGTGGTGCT
ATTGCAGTCCACACAGCATCATCCAACCTGGTACCAAGCCTCTTGGGTCTGTGCATGATT
GATGTTGTAGAAGGTACAGCTATGGATGCACTTAATAGCATGCAGAATTTCTTACGGGGT
CGTCCTAAAACCTTCAAGTCTCTGGAGAATGCTATTGAATGGAGTGTGAAGAGTGGCCAG
ATTCGAAATCTGGAGTCTGCCCGTGTCTCAATGGTTGGCCAAGTCAAACAGTGTGAAGGA
ATTACAAGTCCAGAAGGCTCAAAATCTATAGTGGAAGGAATCATAGAGGAAGAAGAAGAA
GATGAGGAAGGAAGTGAGTCTATAAGCAAGAGGAAAAAGGAAGATGACATGGAGACCAAG
AAAGACCATCCATACACCTGGAGAATTGAACTGGCAAAAACAGAAAAATACTGGGACGGC
TGGTTCCGAGGCTTATCCAATCTCTTTCTTAGTTGTCCCATTCCTAAATTGCTGCTCTTG
GCTGGTGTTGATAGATTGGATAAAGATCTGACCATTGGCCAGATGCAAGGGAAGTTCCAG
ATGCAGGTCCTACCCCAGTGTGGCCATGCAGTCCATGAGGATGCCCCTGACAAGGTAGCT
GAAGCTGTTGCCACTTTCCTGATCCGGCACAGGTTTGCAGAACCCATCGGTGGATTCCAG
TGTGTGTTTCCTGGCTGT

ORF Protein

Cloned ORF protein sequence for pFN21AB9511 Download

>KIBB9511  386 aa
MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVEN
ETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKV
KNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMI
DVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEG
ITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDG
WFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVA
EAVATFLIRHRFAEPIGGFQCVFPGC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9511 Download

>pFN21AB9511  5863 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGCCCTCGAAAAGAGCATGCACCTCGGCCG
CCTTCCCTCTCGCCCACCTCTACCCGGCAGCGGGGGCAGTCAGAGCGGAGCCAAGATGCG
AATGGGCCCTGGAAGAAAGCGGGACTTTTCCCCTGTTCCTTGGAGTCAGTATTTTGAGTC
CATGGAAGATGTAGAAGTAGAGAATGAAACTGGCAAGGATACTTTTCGAGTCTACAAGAG
TGGTTCAGAGGGTCCAGTCCTGCTCCTTCTGCATGGAGGAGGTCATTCTGCCCTTTCTTG
GGCTGTGTTCACGGCAGCGATTATTAGTAGAGTTCAGTGTAGGATTGTAGCTTTGGATCT
GCGAAGTCATGGTGAAACAAAGGTCAAGAATCCTGAAGATCTGTCTGCAGAAACAATGGC
AAAAGACGTTGGCAATGTGGTTGAAGCCATGTATGGGGACCTTCCTCCTCCAATTATGCT
GATTGGACATAGCATGGGTGGTGCTATTGCAGTCCACACAGCATCATCCAACCTGGTACC
AAGCCTCTTGGGTCTGTGCATGATTGATGTTGTAGAAGGTACAGCTATGGATGCACTTAA
TAGCATGCAGAATTTCTTACGGGGTCGTCCTAAAACCTTCAAGTCTCTGGAGAATGCTAT
TGAATGGAGTGTGAAGAGTGGCCAGATTCGAAATCTGGAGTCTGCCCGTGTCTCAATGGT
TGGCCAAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAGGCTCAAAATCTATAGTGGA
AGGAATCATAGAGGAAGAAGAAGAAGATGAGGAAGGAAGTGAGTCTATAAGCAAGAGGAA
AAAGGAAGATGACATGGAGACCAAGAAAGACCATCCATACACCTGGAGAATTGAACTGGC
AAAAACAGAAAAATACTGGGACGGCTGGTTCCGAGGCTTATCCAATCTCTTTCTTAGTTG
TCCCATTCCTAAATTGCTGCTCTTGGCTGGTGTTGATAGATTGGATAAAGATCTGACCAT
TGGCCAGATGCAAGGGAAGTTCCAGATGCAGGTCCTACCCCAGTGTGGCCATGCAGTCCA
TGAGGATGCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGGCACAGGTT
TGCAGAACCCATCGGTGGATTCCAGTGTGTGTTTCCTGGCTGTGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT