Restriction map for
pFN21AB9511
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeI MaeIII MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 510 GGTACC G^GTACC
AccI 205 GTCTAC GT^[AC][GT]AC
AcsI 585 GAATTT [GA]^AATT[CT]
ApaI 99 GGGCCC GGGCC^C
ApoI 585 GAATTT [GA]^AATT[CT]
Asp718I 510 GGTACC G^GTACC
BanI 510 GGTACC G^G[CT][GA]CC
BanII 99 GGGCCC G[GA]GC[CT]^C
BcgI 20 GCATGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 192 GGATAC GTATCC(6/5)
BcnI 58 CCCGG CC^[GC]GG
BmyI 99 GGGCCC G[AGT]GC[ACT]^C
BsaBI 979 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 832 GAGACC GGTCTC(1/5)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmI 627 GAATGC GAATGC(1/-1)
Bsp1286I 99 GGGCCC G[AGT]GC[ACT]^C
BspMI 1023 GCAGGT ACCTGC(4/8)
BsrBI 78 GAGCGG CCGCTC(-3/-3)
BsrDI 407 GCAATG GCAATG(2/0)
BstBI 662 TTCGAA TT^CGAA
BstXI 850 CCATACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 928 CTTAG C^T[ACGT]AG
DsaI 155 CCATGG C^C[GA][CT]GG
EagI 30 CGGCCG C^GGCCG
EarI 648 GAAGAG CTCTTC(1/4)
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
HphI 346 GGTGA GGTGA(8/7)
KpnI 510 GGTACC GGTAC^C
Ksp632I 648 GAAGAG CTCTTC(1/4)
MaeII 401 ACGT A^CGT
MamI 979 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 572 TTAA T^TAA
MslI 1054 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 63 CAGCGG C[AC]G^C[GT]G
NciI 58 CCCGG CC^[GC]GG
NcoI 155 CCATGG C^CATGG
NspV 662 TTCGAA TT^CGAA
PstI 380 CTGCAG CTGCA^G
SexAI 506 ACCTGGT A^CC[AT]GGT
SfuI 662 TTCGAA TT^CGAA
Tru9I 572 TTAA T^TAA
XcmI 86 CCAAGATGCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 511 GTAC GT^AC
554 GTAC
AlwI 1101 GATCC GGATC(4/5)
330 GGATC
BglII 372 AGATCT A^GATCT
984 AGATCT
BseRI 440 CTCCTC GAGGAG(10/8)
251 GAGGAG
Cac8I 20 GCATGC GC[ACGT]^[ACGT]GC
578 GCATGC
EcoT14I 133 CCTTGG C^C[AT][AT]GG
155 CCATGG
FauI 65 GCGGG CCCGC(4/6)
114 GCGGG
HapII 59 CCGG C^CGG
1104 CCGG
HpaII 59 CCGG C^CGG
1104 CCGG
MspI 59 CCGG C^CGG
1104 CCGG
NspI 20 GCATGC [GA]CATG^[CT]
578 GCATGC
PpuMI 431 GGGACCT [GA]G^G[AT]CC[CT]
1025 AGGTCCT
RsaI 511 GTAC GT^AC
554 GTAC
SfcI 380 CTGCAG C^T[GA][CT]AG
746 CTATAG
SphI 20 GCATGC GCATG^C
578 GCATGC
StyI 133 CCTTGG C^C[AT][AT]GG
155 CCATGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AvaII BalI BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboI MboII MflI
MluNI MnlI MscI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XhoII