(Circular) (Six-base) MAPSORT of: pFN21AB9511.seq Check: 7708 from: 1 to: 5863 pFN21AB9511 5863 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2211 3206 1937 Size: 274 995 4594 Fragments arranged by size: 4594 995 274 Acc65I G'GTAC_C Cuts at: 2515 3184 2515 Size: 669 5194 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2394 3105 3926 5310 5578 1632 Size: 762 711 821 1384 268 1917 Fragments arranged by size: 1917 1384 821 762 711 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3135 1184 Size: 1951 3912 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 2891 Size: 32 5831 AlwNI CAG_nnn'CTG Cuts at: 3606 5178 3606 Size: 1572 4291 ApaI G_GGCC'C Cuts at: 2108 2108 Size: 5863 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 2590 3172 3398 3550 5659 1493 Size: 1097 582 226 152 2109 1697 Fragments arranged by size: 2109 1697 1097 582 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 3188 1870 Size: 73 1245 4545 Fragments arranged by size: 4545 1245 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2515 3184 4639 618 Size: 325 217 627 728 669 1455 1842 Fragments arranged by size: 1842 1455 728 669 627 325 217 BanII G_rGCy'C Cuts at: 1391 2108 3182 1391 Size: 717 1074 4072 Fragments arranged by size: 4072 1074 717 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4892 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2014 2048 4054 4088 1903 Size: 34 77 34 2006 34 3678 Fragments arranged by size: 3678 2006 77 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2191 3755 4965 2191 Size: 1564 1210 3089 Fragments arranged by size: 3089 1564 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4474 136 Size: 107 122 71 631 605 337 2465 1525 Fragments arranged by size: 2465 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2377 2989 5858 2377 Size: 612 2869 2382 Fragments arranged by size: 2869 2382 612 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 2108 3924 5080 1790 Size: 318 1816 1156 2573 Fragments arranged by size: 2573 1816 1156 318 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2905 3034 3594 Size: 416 53 83 399 24 1488 129 560 Cuts at: 3594 4552 442 Size: 958 1753 Fragments arranged by size: 1753 1488 958 560 416 399 129 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2648 2696 2884 4523 1239 Size: 1409 48 188 1639 2579 Fragments arranged by size: 2579 1639 1409 188 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3988 4853 5151 3988 Size: 865 298 4700 Fragments arranged by size: 4700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2831 4526 882 Size: 617 1332 1695 2219 Fragments arranged by size: 2219 1695 1332 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 2988 5857 2988 Size: 2869 2994 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5638 Fragments arranged by size: 5638 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2271 2436 1186 Size: 1085 165 4613 Fragments arranged by size: 4613 1085 165 BseYI C'CCAG_C Cuts at: 5066 5562 5066 Size: 496 5367 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 3313 4074 5102 1540 Size: 275 186 37 1275 761 1028 2301 Fragments arranged by size: 2301 1275 1028 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 3924 4009 5080 3924 Size: 85 1071 4707 Fragments arranged by size: 4707 1071 85 BsmI GAATG_Cn' Cuts at: 2638 3374 3467 2638 Size: 736 93 5034 Fragments arranged by size: 5034 736 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 3182 3924 4009 5080 1391 Size: 399 318 1074 742 85 1071 2174 Fragments arranged by size: 2174 1074 1071 742 399 318 85 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3019 3218 844 Size: 2175 199 3489 Fragments arranged by size: 3489 2175 199 BsrBI CCG'CTC Cuts at: 1394 1823 2085 3750 1394 Size: 429 262 1665 3507 Fragments arranged by size: 3507 1665 429 262 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 4358 4532 65 Size: 1183 1171 1939 174 1396 Fragments arranged by size: 1939 1396 1183 1171 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4507 4694 1700 Size: 110 2697 187 2869 Fragments arranged by size: 2869 2697 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5863 BstBI TT'CG_AA Cuts at: 2668 4667 2668 Size: 1999 3864 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2862 2862 Size: 5863 BstYI r'GATC_y Cuts at: 1972 2335 2377 2989 3193 3560 3945 3962 5414 Size: 363 42 612 204 367 385 17 1452 Cuts at: 5414 5500 5512 5858 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 612 385 367 363 346 204 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 513 Size: 552 1095 4216 Fragments arranged by size: 4216 1095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4173 4193 3379 Size: 794 20 5049 Fragments arranged by size: 5049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2659 2701 Size: 54 1425 50 275 18 205 624 42 Cuts at: 2701 3001 3046 3310 4199 5828 8 Size: 300 45 264 889 1629 43 Fragments arranged by size: 1629 1425 889 624 300 275 264 205 54 50 45 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 3310 1537 Size: 275 223 1275 4090 Fragments arranged by size: 4090 1275 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 2648 3793 1353 Size: 1295 1145 3423 Fragments arranged by size: 3423 1295 1145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2394 2648 2696 2884 3105 3926 4523 Size: 393 762 254 48 188 221 821 597 Cuts at: 4523 5310 5578 1239 Size: 787 268 1524 Fragments arranged by size: 1524 821 787 762 597 393 268 254 221 188 48 EcoO109I rG'GnC_Cy Cuts at: 2105 2437 3031 2105 Size: 332 594 4937 Fragments arranged by size: 4937 594 332 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 4504 4536 4578 Size: 32 135 32 1084 32 1816 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1816 1084 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 2519 3188 2519 Size: 669 5194 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 4977 5161 1305 Size: 1227 2445 184 2007 Fragments arranged by size: 2445 2007 1227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2661 2703 3003 3048 10 Size: 54 1425 343 829 42 300 45 2825 Fragments arranged by size: 2825 1425 829 343 300 54 45 42 MslI CAynn'nnrTG Cuts at: 518 1166 3063 3821 4180 4339 518 Size: 648 1897 758 359 159 2042 Fragments arranged by size: 2042 1897 758 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 3279 3956 5104 5349 1606 Size: 464 1209 677 1148 245 2120 Fragments arranged by size: 2120 1209 1148 677 464 245 NcoI C'CATG_G Cuts at: 513 1065 2160 513 Size: 552 1095 4216 Fragments arranged by size: 4216 1095 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2029 2587 3221 4766 1132 Size: 97 800 558 634 1545 2229 Fragments arranged by size: 2229 1545 800 634 558 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 3031 2437 Size: 594 5269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspOMI G'GGCC_C Cuts at: 2104 2104 Size: 5863 PstI C_TGCA'G Cuts at: 830 2389 3215 830 Size: 1559 826 3478 Fragments arranged by size: 3478 1559 826 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SexAI A'CCwGG_T Cuts at: 2511 2511 Size: 5863 SfcI C'TryA_G Cuts at: 826 1045 2385 2751 3211 4346 5027 5218 826 Size: 219 1340 366 460 1135 681 191 1471 Fragments arranged by size: 1471 1340 1135 681 460 366 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3967 4868 5130 820 Size: 197 926 2024 901 262 1553 Fragments arranged by size: 2024 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 2029 2587 3221 1132 Size: 897 558 634 3774 Fragments arranged by size: 3774 897 634 558 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2138 2160 3302 513 Size: 552 1073 22 1142 3074 Fragments arranged by size: 3074 1142 1073 552 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 3890 3907 4060 4245 4690 325 Size: 1255 885 1425 17 153 185 445 1498 Fragments arranged by size: 1498 1425 1255 885 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4109 5821 96 Size: 274 80 33 51 1443 2132 1712 138 Fragments arranged by size: 2132 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2098 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI