Gene/Protein Characteristic Table for KIAA0057
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Product ID ORK00388
Accession No D31762
Description translocation associated membrane protein 2
Clone name ha00909
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (6974 bp)
Predicted protein sequence (384 aa)
Flexi ORF Clone FXC00388
Source Myeloblast cell line (KG-1)
Features of the cloned cDNA sequence
Description

Length: 6974 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 5786 bp
Genome contig ID gi89161210r_52370166
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
ATATTTTCTGAAATAACAATAAAAGGCAGAAAATT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATCAGAATTGTCTCATAATTATTTCCTTGACGTGCTTTTATACAGTGATG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 6 r 52470166 52549747 11 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 384 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q15035 1.4e-166 100.0 Translocation-a...
Homo sapiens
XP_518539 1.7e-166 99.7 translocation-a...
Pan troglodytes
XP_001107590 1.9e-165 99.2 translocation-a...
Macaca mulatta
XP_001499056 6.7e-164 97.8 similar to Tran...
Equus caballus
XP_001928862 1.7e-159 95.4 similar to Tran...
Sus scrofa
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013599 59 125 PF08390 TRAM1-like protein
IPR005547 146 341 PF03798 Longevity-assurance protein (LAG1)
HMMSmart IPR006634 126 335 SM00724 TRAM
ProfileScan IPR006634 126 335 PS50922 TRAM

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 43 LVLCVLIGLMFEVTAKTAFLFIL 65 PRIMARY 23
2 87 KDLVTILFYIFITIILHAVVQEY 109 PRIMARY 23
3 132 GQLVVFHFTSVIWCFYVVVTEGY 154 SECONDARY 23
4 170 LPFQVKFFYLCQLAYWLHALPEL 192 SECONDARY 23
5 209 ICLYLVHIAGAYLLNLSRLGLIL 231 PRIMARY 23
6 262 WAAVFGVTRLFILTLAVLAIGFG 284 PRIMARY 23
7 300 FNTLFCRLCVLLLVCAAQAWLMW 322 PRIMARY 23
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 6
Experimental conditions
Panel name Stanford G3
Primer_f CAGAAGGATGGTGTGAACTCG
Primer_r CTGCTGTTTGGTTTGGTTTTG
PCR product length 273 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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