KIAA0057

Product ID	ORK00388
Accession No	D31762
Description	translocation associated membrane protein 2
Clone name	ha00909
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6974 bp) Predicted protein sequence (384 aa)
HaloTag ORF Clone	FHC00388
Flexi ORF Clone	FXC00388
Source	Myeloblast cell line (KG-1)

Length: 6974 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	5786 bp
Genome contig ID	gi89161210r_52370166
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- ATATTTTCTGAAATAACAATAAAAGGCAGAAAATT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATCAGAATTGTCTCATAATTATTTCCTTGACGTGCTTTTATACAGTGATG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	r	52470166	52549747	11	99.2	Perfect prediction

Length: 384 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q15035	1.4e-166	100.0	Translocation-a...	Homo sapiens
XP_518539	1.7e-166	99.7	translocation-a...	Pan troglodytes
XP_001107590	1.9e-165	99.2	translocation-a...	Macaca mulatta
XP_001499056	6.7e-164	97.8	similar to Tran...	Equus caballus
XP_001928862	1.7e-159	95.4	similar to Tran...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013599	59	125	PF08390	TRAM1-like protein
HMMPfam	IPR005547	146	341	PF03798	Longevity-assurance protein (LAG1)
HMMSmart	IPR006634	126	335	SM00724	TRAM
ProfileScan	IPR006634	126	335	PS50922	TRAM

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	43	LVLCVLIGLMFEVTAKTAFLFIL	65	PRIMARY	23
	2	87	KDLVTILFYIFITIILHAVVQEY	109	PRIMARY	23
	3	132	GQLVVFHFTSVIWCFYVVVTEGY	154	SECONDARY	23
	4	170	LPFQVKFFYLCQLAYWLHALPEL	192	SECONDARY	23
	5	209	ICLYLVHIAGAYLLNLSRLGLIL	231	PRIMARY	23
	6	262	WAAVFGVTRLFILTLAVLAIGFG	284	PRIMARY	23
	7	300	FNTLFCRLCVLLLVCAAQAWLMW	322	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	CAGAAGGATGGTGTGAACTCG
Primer_r	CTGCTGTTTGGTTTGGTTTTG
PCR product length	273 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles