##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII Acc65I AccIII AgeI Aor51HI
AscI Asp718I BglII BseAI BsiWI
BspDI BspEI BspHI BssSI Bst1107I
BstZ17I ClaI CpoI DraIII Eco47III
EcoRI EcoRV FseI HpaI KpnI
MfeI MluI MroI MunI NotI
NruI PacI PinAI PmeI PshAI
PvuI RcaI RsrII SbfI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 3244 AACGTT AA^CGTT
ApaI 45 GGGCCC GGGCC^C
AseI 4432 ATTAAT AT^TAAT
AsnI 4432 ATTAAT AT^TAAT
AviII 1983 TGCGCA TGC^GCA
BamHI 3292 GGATCC G^GATCC
BbrPI 6465 CACGTG CAC^GTG
BbvCI 2718 GCTGAGG CCTCAGC(-5/-2)
BciVI 490 GGATAC GTATCC(6/5)
BclI 6933 TGATCA T^GATCA
BsaBI 1711 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4681 ACCGGA [AT]^CCGG[AT]
Bsp1407I 6915 TGTACA T^GTACA
BsrGI 6915 TGTACA T^GTACA
BssHII 62 GCGCGC G^CGCGC
BstBI 5814 TTCGAA TT^CGAA
DrdI 6420 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4131 ATGCAT ATGCA^T
FbaI 6933 TGATCA T^GATCA
FspI 1983 TGCGCA TGC^GCA
KspI 1903 CCGCGG CCGC^GG
MamI 1711 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 6729 CATATG CA^TATG
NheI 4084 GCTAGC G^CTAGC
NsiI 4131 ATGCAT ATGCA^T
NspV 5814 TTCGAA TT^CGAA
PaeR7I 1880 CTCGAG C^TCGAG
PmaCI 6465 CACGTG CAC^GTG
PmlI 6465 CACGTG CAC^GTG
PshBI 4432 ATTAAT AT^TAAT
Psp1406I 3244 AACGTT AA^CGTT
SacII 1903 CCGCGG CCGC^GG
SalI 2033 GTCGAC G^TCGAC
SfiI 46 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5814 TTCGAA TT^CGAA
SspBI 6915 TGTACA T^GTACA
SspI 6939 AATATT AAT^ATT
XbaI 6449 TCTAGA T^CTAGA
XhoI 1880 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2033 GTCGAC GT^[AC][GT]AC
5449 GTAGAC
AccII 63 CGCG CG^CG
1904 CGCG
AflII 5006 CTTAAG C^TTAAG
6379 CTTAAG
AspI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3324 GACAGAGTC
AvrII 2038 CCTAGG C^CTAGG
3436 CCTAGG
BbeI 1471 GGCGCC GGCGC^C
2406 GGCGCC
BcnI 4411 CCGGG CC^[GC]GG
6116 CCGGG
BfrI 5006 CTTAAG C^TTAAG
6379 CTTAAG
BlnI 2038 CCTAGG C^CTAGG
3436 CCTAGG
BlpI 4201 GCTCAGC GC^T[ACGT]AGC
4494 GCTGAGC
Bpu1102I 4201 GCTCAGC GC^T[ACGT]AGC
4494 GCTGAGC
BsaAI 472 TACGTG [CT]AC^GT[GA]
6465 CACGTG
BsiEI 54 CGGCCG CG[GA][CT]^CG
2540 CGGCCG
BsmBI 1289 CGTCTC CGTCTC(1/5)
3689 GAGACG
BsmI 2092 GCATTC GAATGC(1/-1)
4133 GCATTC
BstAPI 947 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1786 GCAGCCACTGC
BstEII 834 GGTTACC G^GT[ACGT]ACC
1114 GGTTACC
BstPI 834 GGTTACC G^GT[ACGT]ACC
1114 GGTTACC
BstUI 63 CGCG CG^CG
1904 CGCG
CelII 4201 GCTCAGC GC^T[ACGT]AGC
4494 GCTGAGC
EagI 54 CGGCCG C^GGCCG
2540 CGGCCG
EclXI 54 CGGCCG C^GGCCG
2540 CGGCCG
Eco52I 54 CGGCCG C^GGCCG
2540 CGGCCG
EcoO65I 834 GGTTACC G^GT[ACGT]ACC
1114 GGTTACC
HincII 2033 GTCGAC GT[CT]^[GA]AC
4023 GTTGAC
HindII 2033 GTCGAC GT[CT]^[GA]AC
4023 GTTGAC
KasI 1471 GGCGCC G^GCGCC
2406 GGCGCC
MvnI 63 CGCG CG^CG
1904 CGCG
NaeI 52 GCCGGC GCC^GGC
4499 GCCGGC
NarI 1471 GGCGCC GG^CGCC
2406 GGCGCC
NciI 4411 CCGGG CC^[GC]GG
6116 CCGGG
NgoMIV 52 GCCGGC G^CCGGC
4499 GCCGGC
PflFI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3324 GACAGAGTC
PstI 733 CTGCAG CTGCA^G
2210 CTGCAG
ScaI 737 AGTACT AGT^ACT
3739 AGTACT
SexAI 296 ACCTGGT A^CC[AT]GGT
668 ACCTGGT
SfoI 1471 GGCGCC GGC^GCC
2406 GGCGCC
SphI 339 GCATGC GCATG^C
3544 GCATGC
Tth111I 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3324 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI