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Order Kazusa clone(s) from : |
| Product ID | ORK05530 |
|---|---|
| Accession No | AB011094 |
| Description | IQ motif and Sec7 domain 2, transcript variant 1 |
| Clone name | hg01393 |
| Vector information | |
| cDNA sequence | DNA sequence (6022 bp) Predicted protein sequence (1555 aa) |
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HaloTag ORF Clone |
FHC05530
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| Flexi ORF Clone | FXC05530 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0522
by Kazusa Mouse cDNA Project
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Length: 6022 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 1354 bp |
|---|---|
| Genome contig ID | gi89161218r_53178784 |
| PolyA signal sequence (CATAAA,-31) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (288464 - 288415) |
----+----*----+----*----+----*----+----*----+----* |
Length: 1555 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR000048 | 415 | 435 | PF00612 | IQ calmodulin-binding region |
| IPR000904 | 817 | 1008 | PF01369 | SEC7-like | |
| HMMSmart | IPR000904 | 817 | 1008 | SM00222 | SEC7-like |
| IPR001849 | 1039 | 1150 | SM00233 | Pleckstrin-like | |
| ProfileScan | IPR000048 | 414 | 443 | PS50096 | IQ calmodulin-binding region |
| IPR000904 | 813 | 1006 | PS50190 | SEC7-like |
RT-PCR
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Experimental conditions| Primer_f | GAGCACAAGGTCTTTTATGAG |
|---|---|
| Primer_r | ATGGACCCTGGCTGTACGAAG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GAGCACAAGGTCTTTTATGAG |
| Primer_r | ATGGACCCTGGCTGTACGAAG |
| PCR product length | 164 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |