##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AflII Alw44I ApaLI
AscI AseI AsnI Asp718I AspI
AviII BfrI BsaBI BsiEI BsiWI
BspHI Bst1107I BstBI BstZ17I CpoI
DraI DrdI EagI EclXI Eco52I
EcoT22I FspI HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NheI NotI NsiI NspV PacI
PflFI PmeI PshAI PshBI PvuI
RcaI RsrII ScaI SfiI SfuI
SnaBI SpeI SspI SwaI Tru9I
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3110 GTCGAC GT^[AC][GT]AC
AclI 5366 AACGTT AA^CGTT
AhdI 3037 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2634 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3037 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5267 CACGTG CAC^GTG
BciVI 2513 GTATCC GTATCC(6/5)
BclI 5379 TGATCA T^GATCA
BglII 3242 AGATCT A^GATCT
BsaAI 5267 CACGTG [CT]AC^GT[GA]
BsmI 5659 GAATGC GAATGC(1/-1)
BspDI 1519 ATCGAT AT^CGAT
BspLU11I 2882 ACATGT A^CATGT
BstAPI 300 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3216 GGTCACC G^GT[ACGT]ACC
BstPI 3216 GGTCACC G^GT[ACGT]ACC
ClaI 1519 ATCGAT AT^CGAT
DraIII 5233 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3037 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3216 GGTCACC G^GT[ACGT]ACC
FbaI 5379 TGATCA T^GATCA
NdeI 1493 CATATG CA^TATG
NruI 702 TCGCGA TCG^CGA
PaeR7I 5289 CTCGAG C^TCGAG
PflMI 2751 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5267 CACGTG CAC^GTG
PmlI 5267 CACGTG CAC^GTG
Psp1406I 5366 AACGTT AA^CGTT
SalI 3110 GTCGAC G^TCGAC
SapI 3300 GCTCTTC GCTCTTC(1/4)
SbfI 1185 CCTGCAGG CCTGCA^GG
SexAI 3351 ACCTGGT A^CC[AT]GGT
Sse8387I 1185 CCTGCAGG CCTGCA^GG
Van91I 2751 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 5289 CTCGAG C^TCGAG
XmnI 2634 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2651 ACGTGT A^C[GA][CT]GT
2882 ACATGT
AgeI 634 ACCGGT A^CCGGT
2553 ACCGGT
AvrII 58 CCTAGG C^CTAGG
2619 CCTAGG
BcgI 3112 CGACTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3432 CGAGTCCATTGC
BlnI 58 CCTAGG C^CTAGG
2619 CCTAGG
BsmBI 3792 CGTCTC CGTCTC(1/5)
2648 GAGACG
Bsp1407I 2885 TGTACA T^GTACA
4310 TGTACA
BsrDI 3438 CATTGC GCAATG(2/0)
4776 GCAATG
BsrGI 2885 TGTACA T^GTACA
4310 TGTACA
BssHII 87 GCGCGC G^CGCGC
434 GCGCGC
EcoRV 5518 GATATC GAT^ATC
5606 GATATC
FseI 1822 GGCCGGCC GGCCGG^CC
4210 GGCCGGCC
HincII 2953 GTTGAC GT[CT]^[GA]AC
3110 GTCGAC
HindII 2953 GTTGAC GT[CT]^[GA]AC
3110 GTCGAC
PinAI 634 ACCGGT A^CCGGT
2553 ACCGGT
SgrAI 849 CGCCGGCG C[GA]^CCGG[CT]G
2552 CACCGGTG
SphI 4002 GCATGC GCATG^C
5455 GCATGC
SspBI 2885 TGTACA T^GTACA
4310 TGTACA
StuI 2589 AGGCCT AGG^CCT
3881 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI