ROUGE

Gene/Protein Characteristic Table for mKIAA0686

Link to : HUGE \| SWISS-PROT/TrEMBL
Features : DNA sequence \| Protein sequence

Accession No. :	AK129190
Description :	Very large G protein-coupled receptor 1.
Clone Name :	mib39001 [Vector Info]
Source :	Mouse adult pancreatic islet
Note :	We replaced mpm11221, former representative clones for mKIAA0686 with mib39001. (2004/6/22)

Features of the cloned DNA sequence	Description

Length: 6589 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 314 bp

Genome contig ID gi65535943r_77071391
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
AATGAAAATAATCATCAATAAAAGTATTGGAATCC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGGTGGTAGCATCCAGCCTCATTATTTATGAATAAATGTGTGGGGGCAAA

Genomic structure deduced by Blast search against draft genome sequences
Comparison with the corresponding KIAA cDNA.

KIAA Alignment based on:

KIAA0686 DNA sequence, AA sequence, Physical map

KIAA	Alignment based on:
KIAA0686	DNA sequence, AA sequence, Physical map

Features of the protein sequence	Description

Coding region: 1824..6275
Length: 1483 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EDL37137	100.0	mCG121994, isof...	Mus musculus
CAX16027	100.0	G protein-coupl...	Mus musculus
Q8VHN7	99.3	G-protein coupl...	Mus musculus
XP_001056150	90.2	similar to very...	Rattus norvegicus
XP_517658	80.6	G protein-coupl...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other ROUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	ROUGE_ID
AK122380	2.7e-14	23.8	mKIAA0821
AK122367	7.1e-14	21.5	mKIAA0768
AK129215	1.6e-13	22.1	mKIAA0786
AK220481	8.7e-09	23.5	mKIAA4089
AK220331	1.1e-08	23.8	mKIAA0279

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR003644 174 274 PF03160 Na-Ca exchanger/integrin-beta4

IPR000832 1087 1337 PF00002 GPCR

HMMSmart IPR003644 174 274 SM00237 Na-Ca exchanger/integrin-beta4

ProfileScan IPR000203 1031 1080 PS50221 GPS

IPR000832 1083 1334 PS50261 GPCR

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003644	174	274	PF03160	Na-Ca exchanger/integrin-beta4
IPR000832	1087	1337	PF00002	GPCR
HMMSmart	IPR003644	174	274	SM00237	Na-Ca exchanger/integrin-beta4
ProfileScan	IPR000203	1031	1080	PS50221	GPS
IPR000832	1083	1334	PS50261	GPCR

Prediction of transmembrane (TM) segments

Method From To amino acid sequence

SOSUI 24 46 GSNFTLQLVSVRLLSGPFYGIPT

1086 1108 AFFSAGLICISGLCLAVVSHMFC

1116 1138 AKLLTHMMVASLGTQILFLASAY

1153 1175 AVAHYLYLCQFSWMLIQSVNFWY

1189 1211 LLFCLLSWGLPSFVVILLILILR

1233 1255 PNIYAALFTAALVPLMCLVVVFV

1278 1300 TNAAEIPLILYLFALISMTWLWG

1312 1334 LVLFVIFNSLQGLYVFVVYFILH

Method	From	To	amino acid sequence
SOSUI	24	46	GSNFTLQLVSVRLLSGPFYGIPT
1086	1108	AFFSAGLICISGLCLAVVSHMFC
1116	1138	AKLLTHMMVASLGTQILFLASAY
1153	1175	AVAHYLYLCQFSWMLIQSVNFWY
1189	1211	LLFCLLSWGLPSFVVILLILILR
1233	1255	PNIYAALFTAALVPLMCLVVVFV
1278	1300	TNAAEIPLILYLFALISMTWLWG
1312	1334	LVLFVIFNSLQGLYVFVVYFILH

How to obtain mKIAA clone(s) Back to the ROUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	YES

Length of 3'UTR	314 bp
Genome contig ID	gi65535943r_77071391
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- AATGAAAATAATCATCAATAAAAGTATTGGAATCC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGGTGGTAGCATCCAGCCTCATTATTTATGAATAAATGTGTGGGGGCAAA

Gene/Protein Characteristic Table for mKIAA0686

Features of the cloned DNA sequence

Features of the protein sequence