################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AscI Asp718I AviII AvrII BfrI BlnI BseAI BsiEI BsiWI BspEI BssHII DrdI EagI EclXI Eco52I FspI KpnI KspI MluI MroI NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI SacII SalI SbfI SexAI SfiI SgrAI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AhdI 5055 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3978 AGCGCT AGC^GCT ApaI 742 GGGCCC GGGCC^C AspEI 5055 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1732 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 975 GGATCC G^GATCC BbeI 563 GGCGCC GGCGC^C BbrPI 367 CACGTG CAC^GTG BcgI 2486 CGAGACAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 6247 GGATAC GTATCC(6/5) BglI 3320 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmBI 818 GAGACG CGTCTC(1/5) BspDI 487 ATCGAT AT^CGAT BspHI 3940 TCATGA T^CATGA Bst1107I 292 GTATAC GTA^TAC BstAPI 2496 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 292 GTATAC GTA^TAC ClaI 487 ATCGAT AT^CGAT CpoI 694 CGGACCG CG^G[AT]CCG Eam1105I 5055 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3978 AGCGCT AGC^GCT FauI 1344 CCCGC CCCGC(4/6) FseI 2262 GGCCGGCC GGCCGG^CC HgaI 1198 GACGC GACGC(5/10) HpaI 3951 GTTAAC GTT^AAC KasI 563 GGCGCC G^GCGCC NarI 563 GGCGCC GG^CGCC PflFI 1732 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 367 CACGTG CAC^GTG PmlI 367 CACGTG CAC^GTG RcaI 3940 TCATGA T^CATGA RsrII 694 CGGACCG CG^G[AT]CCG ScaI 3069 AGTACT AGT^ACT SfoI 563 GGCGCC GGC^GCC SnaBI 418 TACGTA TAC^GTA SspI 6510 AATATT AAT^ATT Tth111I 1732 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 3765 GACGTC GACGT^C 5637 GACGTC Alw44I 1111 GTGCAC G^TGCAC 4840 GTGCAC ApaLI 1111 GTGCAC G^TGCAC 4840 GTGCAC AseI 2595 ATTAAT AT^TAAT 2634 ATTAAT AsnI 2595 ATTAAT AT^TAAT 2634 ATTAAT BsaWI 697 ACCGGA [AT]^CCGG[AT] 3646 TCCGGT BsgI 1286 GTGCAG GTGCAG(16/14) 4078 GTGCAG BsmI 1979 GAATGC GAATGC(1/-1) 3219 GCATTC BspLU11I 5023 ACATGT A^CATGT 5137 ACATGT BsrFI 2064 GCCGGC [GA]^CCGG[CT] 2263 GCCGGC Cfr10I 2064 GCCGGC [GA]^CCGG[CT] 2263 GCCGGC DraI 4118 TTTAAA TTT^AAA 6441 TTTAAA EcoRI 119 GAATTC G^AATTC 3333 GAATTC EcoRV 1102 GATATC GAT^ATC 3078 GATATC HincII 2796 GTTGAC GT[CT]^[GA]AC 3951 GTTAAC HindII 2796 GTTGAC GT[CT]^[GA]AC 3951 GTTAAC MfeI 3246 CAATTG C^AATTG 6253 CAATTG MunI 3246 CAATTG C^AATTG 6253 CAATTG NaeI 2064 GCCGGC GCC^GGC 2263 GCCGGC NcoI 2362 CCATGG C^CATGG 2815 CCATGG NdeI 5233 CATATG CA^TATG 5730 CATATG NgoMIV 2064 GCCGGC G^CCGGC 2263 GCCGGC PshBI 2595 ATTAAT AT^TAAT 2634 ATTAAT SapI 4605 GCTCTTC GCTCTTC(1/4) 5390 GCTCTTC SmaI 389 CCCGGG CCC^GGG 6089 CCCGGG SphI 4728 GCATGC GCATG^C 5473 GCATGC StuI 2294 AGGCCT AGG^CCT 3485 AGGCCT XmaCI 389 CCCGGG C^CCGGG 6089 CCCGGG XmaI 389 CCCGGG C^CCGGG 6089 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 146 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BssSI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI NspV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI