##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI Asp718I AviII AvrII BfrI
BlnI BseAI BsiEI BsiWI BspEI
BssHII DrdI EagI EclXI Eco52I
FspI KpnI KspI MluI MroI
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
SacII SalI SbfI SexAI SfiI
SgrAI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AhdI 5055 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3978 AGCGCT AGC^GCT
ApaI 742 GGGCCC GGGCC^C
AspEI 5055 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1732 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 975 GGATCC G^GATCC
BbeI 563 GGCGCC GGCGC^C
BbrPI 367 CACGTG CAC^GTG
BcgI 2486 CGAGACAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6247 GGATAC GTATCC(6/5)
BglI 3320 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 818 GAGACG CGTCTC(1/5)
BspDI 487 ATCGAT AT^CGAT
BspHI 3940 TCATGA T^CATGA
Bst1107I 292 GTATAC GTA^TAC
BstAPI 2496 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 292 GTATAC GTA^TAC
ClaI 487 ATCGAT AT^CGAT
CpoI 694 CGGACCG CG^G[AT]CCG
Eam1105I 5055 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3978 AGCGCT AGC^GCT
FauI 1344 CCCGC CCCGC(4/6)
FseI 2262 GGCCGGCC GGCCGG^CC
HgaI 1198 GACGC GACGC(5/10)
HpaI 3951 GTTAAC GTT^AAC
KasI 563 GGCGCC G^GCGCC
NarI 563 GGCGCC GG^CGCC
PflFI 1732 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 367 CACGTG CAC^GTG
PmlI 367 CACGTG CAC^GTG
RcaI 3940 TCATGA T^CATGA
RsrII 694 CGGACCG CG^G[AT]CCG
ScaI 3069 AGTACT AGT^ACT
SfoI 563 GGCGCC GGC^GCC
SnaBI 418 TACGTA TAC^GTA
SspI 6510 AATATT AAT^ATT
Tth111I 1732 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 3765 GACGTC GACGT^C
5637 GACGTC
Alw44I 1111 GTGCAC G^TGCAC
4840 GTGCAC
ApaLI 1111 GTGCAC G^TGCAC
4840 GTGCAC
AseI 2595 ATTAAT AT^TAAT
2634 ATTAAT
AsnI 2595 ATTAAT AT^TAAT
2634 ATTAAT
BsaWI 697 ACCGGA [AT]^CCGG[AT]
3646 TCCGGT
BsgI 1286 GTGCAG GTGCAG(16/14)
4078 GTGCAG
BsmI 1979 GAATGC GAATGC(1/-1)
3219 GCATTC
BspLU11I 5023 ACATGT A^CATGT
5137 ACATGT
BsrFI 2064 GCCGGC [GA]^CCGG[CT]
2263 GCCGGC
Cfr10I 2064 GCCGGC [GA]^CCGG[CT]
2263 GCCGGC
DraI 4118 TTTAAA TTT^AAA
6441 TTTAAA
EcoRI 119 GAATTC G^AATTC
3333 GAATTC
EcoRV 1102 GATATC GAT^ATC
3078 GATATC
HincII 2796 GTTGAC GT[CT]^[GA]AC
3951 GTTAAC
HindII 2796 GTTGAC GT[CT]^[GA]AC
3951 GTTAAC
MfeI 3246 CAATTG C^AATTG
6253 CAATTG
MunI 3246 CAATTG C^AATTG
6253 CAATTG
NaeI 2064 GCCGGC GCC^GGC
2263 GCCGGC
NcoI 2362 CCATGG C^CATGG
2815 CCATGG
NdeI 5233 CATATG CA^TATG
5730 CATATG
NgoMIV 2064 GCCGGC G^CCGGC
2263 GCCGGC
PshBI 2595 ATTAAT AT^TAAT
2634 ATTAAT
SapI 4605 GCTCTTC GCTCTTC(1/4)
5390 GCTCTTC
SmaI 389 CCCGGG CCC^GGG
6089 CCCGGG
SphI 4728 GCATGC GCATG^C
5473 GCATGC
StuI 2294 AGGCCT AGG^CCT
3485 AGGCCT
XmaCI 389 CCCGGG C^CCGGG
6089 CCCGGG
XmaI 389 CCCGGG C^CCGGG
6089 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BssSI BstBI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI NspV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI