ROUGE

Gene/Protein Characteristic Table for mKIAA0099

Link to : HUGE \| SWISS-PROT/TrEMBL
Features : DNA sequence \| Protein sequence

Accession No. :	AK122205
Description :	Pumilio homolog 1.
Clone Name :	mbg05872 [Vector Info]
Source :	Mouse brain

Features of the cloned DNA sequence	Description

Length: 5329 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 1659 bp

Genome contig ID gi65493515f_129360265
PolyA signal sequence
(AATACA,-21)
+----*----+----*----+----*----+----
TTTATTTTGAAAAAAATACAGAACTTTGGAATCTG
Flanking genome sequence
(218185 - 218234)
----+----*----+----*----+----*----+----*----+----*
AATTTGTACTGCTCTCACTTCTCAAACCTGCTCAGGCAAGCCCTGCTCTA

Genomic structure deduced by Blast search against draft genome sequences
Comparison with the corresponding KIAA cDNA.

KIAA Alignment based on:

KIAA0099 DNA sequence, AA sequence

KIAA	Alignment based on:
KIAA0099	DNA sequence, AA sequence

Features of the protein sequence	Description

Coding region: 14..3670
Length: 1218 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EDL30136	99.8	pumilio 1 (Dros...	Mus musculus
EDL30135	99.9	pumilio 1 (Dros...	Mus musculus
Q80U78	100.0	Pumilio homolog 1.	Mus musculus
BAE24888	99.9	unnamed protein...	Mus musculus
AAH48174	99.9	Pumilio 1 (Dros...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other ROUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	ROUGE_ID
AK122225	3.7e-76	71.9	mKIAA0235
AK172872	0.00094	20.5	mKIAA0020

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR000104 472 486 PR00308 Antifreeze protein

IPR000104 507 516 PR00308 Antifreeze protein

HMMPfam IPR001313 878 912 PF00806 Pumilio/Puf RNA-binding

IPR001313 914 948 PF00806 Pumilio/Puf RNA-binding

IPR001313 950 985 PF00806 Pumilio/Puf RNA-binding

IPR001313 988 1022 PF00806 Pumilio/Puf RNA-binding

IPR001313 1024 1058 PF00806 Pumilio/Puf RNA-binding

IPR001313 1060 1094 PF00806 Pumilio/Puf RNA-binding

IPR001313 1096 1131 PF00806 Pumilio/Puf RNA-binding

IPR001313 1139 1173 PF00806 Pumilio/Puf RNA-binding

HMMSmart IPR001313 878 913 SM00025 Pumilio/Puf RNA-binding

IPR001313 914 949 SM00025 Pumilio/Puf RNA-binding

IPR001313 950 984 SM00025 Pumilio/Puf RNA-binding

IPR001313 988 1023 SM00025 Pumilio/Puf RNA-binding

IPR001313 1024 1059 SM00025 Pumilio/Puf RNA-binding

IPR001313 1060 1095 SM00025 Pumilio/Puf RNA-binding

IPR001313 1096 1131 SM00025 Pumilio/Puf RNA-binding

IPR001313 1139 1174 SM00025 Pumilio/Puf RNA-binding

ProfileScan NULL 422 643 PS50310 NULL

NULL 505 553 PS50322 NULL

NULL 672 845 PS50324 NULL

IPR001313 878 913 PS50303 Pumilio/Puf RNA-binding

IPR001313 878 1196 PS50302 Pumilio/Puf RNA-binding

IPR001313 914 949 PS50303 Pumilio/Puf RNA-binding

IPR001313 950 987 PS50303 Pumilio/Puf RNA-binding

IPR001313 988 1023 PS50303 Pumilio/Puf RNA-binding

IPR001313 1024 1059 PS50303 Pumilio/Puf RNA-binding

IPR001313 1060 1095 PS50303 Pumilio/Puf RNA-binding

IPR001313 1096 1131 PS50303 Pumilio/Puf RNA-binding

IPR001313 1135 1174 PS50303 Pumilio/Puf RNA-binding

Prediction of transmembrane (TM) segments

Method From To amino acid sequence

- - - -

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000104	472	486	PR00308	Antifreeze protein
IPR000104	507	516	PR00308	Antifreeze protein
HMMPfam	IPR001313	878	912	PF00806	Pumilio/Puf RNA-binding
IPR001313	914	948	PF00806	Pumilio/Puf RNA-binding
IPR001313	950	985	PF00806	Pumilio/Puf RNA-binding
IPR001313	988	1022	PF00806	Pumilio/Puf RNA-binding
IPR001313	1024	1058	PF00806	Pumilio/Puf RNA-binding
IPR001313	1060	1094	PF00806	Pumilio/Puf RNA-binding
IPR001313	1096	1131	PF00806	Pumilio/Puf RNA-binding
IPR001313	1139	1173	PF00806	Pumilio/Puf RNA-binding
HMMSmart	IPR001313	878	913	SM00025	Pumilio/Puf RNA-binding
IPR001313	914	949	SM00025	Pumilio/Puf RNA-binding
IPR001313	950	984	SM00025	Pumilio/Puf RNA-binding
IPR001313	988	1023	SM00025	Pumilio/Puf RNA-binding
IPR001313	1024	1059	SM00025	Pumilio/Puf RNA-binding
IPR001313	1060	1095	SM00025	Pumilio/Puf RNA-binding
IPR001313	1096	1131	SM00025	Pumilio/Puf RNA-binding
IPR001313	1139	1174	SM00025	Pumilio/Puf RNA-binding
ProfileScan	NULL	422	643	PS50310	NULL
NULL	505	553	PS50322	NULL
NULL	672	845	PS50324	NULL
IPR001313	878	913	PS50303	Pumilio/Puf RNA-binding
IPR001313	878	1196	PS50302	Pumilio/Puf RNA-binding
IPR001313	914	949	PS50303	Pumilio/Puf RNA-binding
IPR001313	950	987	PS50303	Pumilio/Puf RNA-binding
IPR001313	988	1023	PS50303	Pumilio/Puf RNA-binding
IPR001313	1024	1059	PS50303	Pumilio/Puf RNA-binding
IPR001313	1060	1095	PS50303	Pumilio/Puf RNA-binding
IPR001313	1096	1131	PS50303	Pumilio/Puf RNA-binding
IPR001313	1135	1174	PS50303	Pumilio/Puf RNA-binding

Method	From	To	amino acid sequence
-	-	-	-

How to obtain mKIAA clone(s) How to obtain anti mKIAA antibodies Back to the ROUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	YES

Length of 3'UTR	1659 bp
Genome contig ID	gi65493515f_129360265
PolyA signal sequence (AATACA,-21)	+--------+--------+----*----+---- TTTATTTTGAAAAAAATACAGAACTTTGGAATCTG
Flanking genome sequence (218185 - 218234)	----+--------+--------+--------+--------+----* AATTTGTACTGCTCTCACTTCTCAAACCTGCTCAGGCAAGCCCTGCTCTA

Gene/Protein Characteristic Table for mKIAA0099

Features of the cloned DNA sequence

Features of the protein sequence